PMID- 28791393 OWN - NLM STAT- MEDLINE DCOM- 20180521 LR - 20211204 IS - 1791-3004 (Electronic) IS - 1791-2997 (Linking) VI - 16 IP - 4 DP - 2017 Oct TI - Elevated expression of yes‑associated protein is associated with the malignant status and prognosis of laryngeal squamous cell carcinoma. PG - 4934-4940 LID - 10.3892/mmr.2017.7187 [doi] AB - Previous studies have demonstrated that elevated yes‑associated protein (YAP) expression is associated with tumor aggression and poor prognosis in various types of human cancer. However, the clinicopathological significance and the prognostic value of YAP in laryngeal squamous cell carcinoma (LSCC) is unknown. The aim of the present study was to identify the expression pattern and prognostic significance of YAP in patients with LSCC. YAP mRNA and protein expression levels were examined in fresh and archived LSCC samples using the reverse transcription‑quantitative polymerase chain reaction, immunohistochemistry (IHC) and western blotting. The association between YAP expression levels with the malignant status and prognosis of patients with LSCC was analyzed. Upregulated protein and mRNA expression levels of YAP were detected in LSCC tissues compared with paired healthy surgical margin tissues. Positive expression of YAP was identified in 84/121 (69.4%) LSCC tissues and in 4/30 (13.3%) healthy surgical margin tissues by IHC. Positive YAP protein expression was significantly associated with clinical stage, TNM classification, lymph node metastasis and differentiated degree. Patients with positive YAP expression exhibited a significantly decreased overall survival time compared with patients with negative YAP expression (P=0.0002). Multivariate analysis indicated that the level of YAP expression was an independent prognostic factor for poor survival in patients with LSCC (P=0.012). In conclusion, the expression level of YAP was significantly increased in LSCC and associated with the malignant status of LSCC. Therefore, YAP may represent a novel biomarker for predicting the prognosis of patients with LSCC. FAU - Pan, Chunchen AU - Pan C AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Du, Zhihui AU - Du Z AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Cai, Zhaogen AU - Cai Z AD - Department of Pathology, The First Hospital Affiliated to Bengbu Medical College, Bengbu, Anhui 233000, P.R. China. FAU - Liu, Yun AU - Liu Y AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Sun, Yanbo AU - Sun Y AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Chen, Jin AU - Chen J AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Tong, Ting AU - Tong T AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Chen, Qingguo AU - Chen Q AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Zhou, Liangqiang AU - Zhou L AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Bing, Dan AU - Bing D AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Tao, Yanling AU - Tao Y AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. FAU - Chu, Hanqi AU - Chu H AD - Department of Otolaryngology‑Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China. LA - eng PT - Journal Article DEP - 20170807 PL - Greece TA - Mol Med Rep JT - Molecular medicine reports JID - 101475259 RN - 0 (Adaptor Proteins, Signal Transducing) RN - 0 (Biomarkers, Tumor) RN - 0 (Phosphoproteins) RN - 0 (Transcription Factors) RN - 0 (YAP-Signaling Proteins) RN - 0 (YAP1 protein, human) SB - IM MH - Adaptor Proteins, Signal Transducing/*genetics/metabolism MH - Adult MH - Aged MH - Aged, 80 and over MH - Biomarkers, Tumor MH - Carcinoma, Squamous Cell/*genetics/mortality/*pathology MH - Female MH - Humans MH - Immunohistochemistry MH - Kaplan-Meier Estimate MH - Laryngeal Neoplasms/*genetics/mortality/*pathology MH - Male MH - Middle Aged MH - Neoplasm Grading MH - Neoplasm Metastasis MH - Neoplasm Staging MH - Phosphoproteins/*genetics/metabolism MH - Prognosis MH - Transcription Factors MH - YAP-Signaling Proteins EDAT- 2017/08/10 06:00 MHDA- 2018/05/22 06:00 CRDT- 2017/08/10 06:00 PHST- 2016/02/09 00:00 [received] PHST- 2017/02/20 00:00 [accepted] PHST- 2017/08/10 06:00 [pubmed] PHST- 2018/05/22 06:00 [medline] PHST- 2017/08/10 06:00 [entrez] AID - 10.3892/mmr.2017.7187 [doi] PST - ppublish SO - Mol Med Rep. 2017 Oct;16(4):4934-4940. doi: 10.3892/mmr.2017.7187. Epub 2017 Aug 7.