PMID- 28791411
OWN - NLM
STAT- MEDLINE
DCOM- 20180510
LR  - 20201209
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 38
IP  - 4
DP  - 2017 Oct
TI  - MicroRNA-195 inhibits growth and invasion of laryngeal carcinoma cells by 
      directly targeting DCUN1D1.
PG  - 2155-2165
LID - 10.3892/or.2017.5875 [doi]
AB  - MicroRNAs (miRNAs) are a class of small, non-coding RNAs that regulate gene 
      expression and are involved in cell biological processes. The aberrant expression 
      of miR-195 has been found in various types of human cancer. However, the effect 
      of miR‑195 on the initiation and development of laryngeal squamous cell carcinoma 
      (LSCC) remains to be elucidated. Accordingly, in the present study, we detected 
      the expression level of miR-195 in the LSCC and the normal tissues and found that 
      miR-195 were significantly down-regulated in the LSCC tissues. Gain-of-function 
      or loss-of-function studies including cell proliferation, wound healing assay, 
      Transwell assay, cell cycle and apoptosis assays were performed to investigate 
      the biological function of miR-195. Luciferase reporter assay and the rescue 
      study confirmed that DCUN1D1 was a target of miR-195. Furthermore, DCUN1D1 
      expression levels were found to be upregulated in laryngeal tissues and to have a 
      negative correlation with miR-195. We also found that both miR-195 and DCUN1D1 
      siRNAs can inhibit cell invasion possibly through downregulating Matrix 
      metalloproteinase-2 (MMP-2) and Matrix metalloproteinase-9 (MMP-9) at the 
      post-transcriptional level, which can be attenuated by restoring the expression 
      of DCUN1D1. In summary, these data suggest that low expression of miR-195 
      contributes to the poor prognosis of LSCC and miR-195 regulates the proliferation 
      and invasion ability of LSCC cells in vitro. miR-195 may suppress growth and 
      invasion of LSCC cells possibly through targeting DCUN1D1, which would provide a 
      candidate target for cancer therapy.
FAU - Shuang, Yu
AU  - Shuang Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Hospital of 
      Tianjin Medical University, Tianjin 300211, P.R. China.
FAU - Li, Chao
AU  - Li C
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Hospital of 
      Tianjin Medical University, Tianjin 300211, P.R. China.
FAU - Zhou, Xuan
AU  - Zhou X
AD  - Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical 
      University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and 
      Therapy, Tianjin Cancer Institute; National Clinical Research Center of Cancer; 
      Tianjin 300211, P.R. China.
FAU - Huang, Yongwang
AU  - Huang Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Hospital of 
      Tianjin Medical University, Tianjin 300211, P.R. China.
FAU - Zhang, Lun
AU  - Zhang L
AD  - Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical 
      University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and 
      Therapy, Tianjin Cancer Institute; National Clinical Research Center of Cancer; 
      Tianjin 300211, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170803
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (DCUN1D1 protein, human)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (MIRN195 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Apoptosis/genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Cycle/genetics
MH  - Cell Proliferation/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Matrix Metalloproteinase 2/genetics
MH  - Matrix Metalloproteinase 9/genetics
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics
MH  - Proteins
MH  - Proto-Oncogene Proteins/*genetics
MH  - Squamous Cell Carcinoma of Head and Neck
PMC - PMC5652960
EDAT- 2017/08/10 06:00
MHDA- 2018/05/11 06:00
PMCR- 2017/08/03
CRDT- 2017/08/10 06:00
PHST- 2017/02/04 00:00 [received]
PHST- 2017/07/25 00:00 [accepted]
PHST- 2017/08/10 06:00 [pubmed]
PHST- 2018/05/11 06:00 [medline]
PHST- 2017/08/10 06:00 [entrez]
PHST- 2017/08/03 00:00 [pmc-release]
AID - or-38-04-2155 [pii]
AID - 10.3892/or.2017.5875 [doi]
PST - ppublish
SO  - Oncol Rep. 2017 Oct;38(4):2155-2165. doi: 10.3892/or.2017.5875. Epub 2017 Aug 3.