PMID- 28792420
OWN - NLM
STAT- MEDLINE
DCOM- 20171103
LR  - 20171103
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 125
IP  - 3
DP  - 2017 Aug 9
TI  - Primary culture of Zhikong scallop Chlamys farreri hemocytes as an in vitro model 
      for studying host-pathogen interactions.
PG  - 217-226
LID - 10.3354/dao03145 [doi]
AB  - Primary cultured cells can be a useful tool in studies on physiology, virology, 
      and toxicology. Hemocytes play an important role in animal rapid response to 
      pathogen invasion. In this study, an appropriate medium for primary culture of 
      hemocytes of the bivalve Chlamys farreri was developed by adding 5% fetal bovine 
      serum and 1% C. farreri serum to Leibovitz L-15 medium. These primary cultured 
      hemocytes were maintained for more than 40 d in vitro and were classified into 3 
      types: (1) granulocytes containing numerous granules in the cytoplasm, (2) 
      hyalinocytes with no or few granules, (3) a small percentage of macrophage-like 
      cells. Furthermore, the primary cultured hemocytes were observed to be sensitive 
      to bacterial and viral challenges. These hemocytes could phagocytose the 
      bacterium Vibrio anguillarum, and presented cytopathic effects on the 
      extracellular products (ECPs) of V. anguillarum; the mRNA level of QM, which 
      plays an important role in immune response, also significantly increased 12 h 
      after infection. When these hemocytes were challenged with ostreid herpesvirus 1 
      (OsHV-1), virus particles and empty capsids in the cells infected for 48 h were 
      observed by transmission electron microscopy, and the QM mRNA level increased 
      significantly at 12 h and 24 h following OsHV-1 challenge. This primary culture 
      system is available for C. farreri hemocytes which can be used in the future to 
      study host-pathogen interactions.
FAU - Ji, Aichang
AU  - Ji A
AD  - Key Laboratory of Marine Genetics and Breeding (Ocean University of China), 
      Ministry of Education, Qingdao 266003, PR China.
FAU - Li, Xueyu
AU  - Li X
FAU - Fang, Sha
AU  - Fang S
FAU - Qin, Zhenkui
AU  - Qin Z
FAU - Bai, Changming
AU  - Bai C
FAU - Wang, Chongming
AU  - Wang C
FAU - Zhang, Zhifeng
AU  - Zhang Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Gene Expression Regulation/immunology
MH  - Hemocytes/microbiology/*physiology
MH  - Herpesviridae/*physiology
MH  - Host-Pathogen Interactions
MH  - Pectinidae/*cytology
MH  - RNA, Viral
MH  - Vibrio/*physiology
MH  - Virus Replication
OTO - NOTNLM
OT  - Cell culture
OT  - Chlamys farreri
OT  - Hemocyte
OT  - Ostreid herpesvirus 1
OT  - Vibrio anguillarum
EDAT- 2017/08/10 06:00
MHDA- 2017/11/04 06:00
CRDT- 2017/08/10 06:00
PHST- 2017/08/10 06:00 [entrez]
PHST- 2017/08/10 06:00 [pubmed]
PHST- 2017/11/04 06:00 [medline]
AID - 10.3354/dao03145 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2017 Aug 9;125(3):217-226. doi: 10.3354/dao03145.