PMID- 28792466 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 2076-393X (Print) IS - 2076-393X (Electronic) IS - 2076-393X (Linking) VI - 5 IP - 3 DP - 2017 Aug 9 TI - Distinctive Responses in an In Vitro Human Dendritic Cell-Based System upon Stimulation with Different Influenza Vaccine Formulations. LID - 10.3390/vaccines5030021 [doi] LID - 21 AB - Vaccine development relies on testing vaccine candidates in animal models. However, results from animals cannot always be translated to humans. Alternative ways to screen vaccine candidates before clinical trials are therefore desirable. Dendritic cells (DCs) are the main orchestrators of the immune system and the link between innate and adaptive responses. Their activation by vaccines is an essential step in vaccine-induced immune responses. We have systematically evaluated the suitability of two different human DC-based systems, namely the DC-cell line MUTZ-3 and primary monocyte-derived DCs (Mo-DCs) to screen immunopotentiating properties of vaccine candidates. Two different influenza vaccine formulations, whole inactivated virus (WIV) and subunit (SU), were used as model antigens as they represent a high immunogenic and low immunogenic vaccine, respectively. MUTZ-3 cells were restricted in their ability to respond to different stimuli. In contrast, Mo-DCs readily responded to WIV and SU in a vaccine-specific way. WIV stimulation elicited a more vigorous induction of activation markers, immune response-related genes and secretion of cytokines involved in antiviral responses than the SU vaccine. Furthermore, Mo-DCs differentiated from freshly isolated and freeze/thawed peripheral blood mononuclear cells (PBMCs) showed a similar capacity to respond to different vaccines. Taken together, we identified human PBMC-derived Mo-DCs as a suitable platform to evaluate vaccine-induced immune responses. Importantly, we show that fresh and frozen PBMCs can be used indistinctly, which strongly facilitates the routine use of this system. In vitro vaccine pre-screening using human Mo-DCs is thus a promising approach for evaluating the immunopotentiating capacities of new vaccine formulations that have not yet been tested in humans. FAU - Tapia-Calle, Gabriela AU - Tapia-Calle G AD - Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, 9713AV Groningen, The Netherlands. m.g.tapia.calle@umcg.nl. FAU - Stoel, Maaike AU - Stoel M AD - Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, 9713AV Groningen, The Netherlands. maaikestoel@gmail.com. FAU - de Vries-Idema, Jacqueline AU - de Vries-Idema J AD - Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, 9713AV Groningen, The Netherlands. j.j.de.vries-idema@umcg.nl. FAU - Huckriede, Anke AU - Huckriede A AD - Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, 9713AV Groningen, The Netherlands. a.l.w.huckriede@umcg.nl. LA - eng PT - Journal Article DEP - 20170809 PL - Switzerland TA - Vaccines (Basel) JT - Vaccines JID - 101629355 PMC - PMC5620552 OTO - NOTNLM OT - MUTZ-3 OT - cytokines OT - dendritic cell OT - flow cytometry OT - qPCR OT - subunit influenza vaccine OT - whole inactivated virus influenza vaccine COIS- The authors declare no conflict of interest. EDAT- 2017/08/10 06:00 MHDA- 2017/08/10 06:01 PMCR- 2017/08/09 CRDT- 2017/08/10 06:00 PHST- 2017/05/03 00:00 [received] PHST- 2017/07/29 00:00 [revised] PHST- 2017/08/02 00:00 [accepted] PHST- 2017/08/10 06:00 [entrez] PHST- 2017/08/10 06:00 [pubmed] PHST- 2017/08/10 06:01 [medline] PHST- 2017/08/09 00:00 [pmc-release] AID - vaccines5030021 [pii] AID - vaccines-05-00021 [pii] AID - 10.3390/vaccines5030021 [doi] PST - epublish SO - Vaccines (Basel). 2017 Aug 9;5(3):21. doi: 10.3390/vaccines5030021.