PMID- 28796322
OWN - NLM
STAT- MEDLINE
DCOM- 20181022
LR  - 20181022
IS  - 1552-8618 (Electronic)
IS  - 0730-7268 (Linking)
VI  - 37
IP  - 1
DP  - 2018 Jan
TI  - Ecotoxicological properties of ketoprofen and the S(+)-enantiomer 
      (dexketoprofen): Bioassays in freshwater model species and biomarkers in fish 
      PLHC-1 cell line.
PG  - 201-212
LID - 10.1002/etc.3943 [doi]
AB  - The increased use of nonsteroidal anti-inflammatory drugs (NSAIDs) has resulted 
      in their ubiquitous presence in the environment. The toxicological properties of 
      these 2 widely prescribed NSAIDs, namely racemic ketoprofen and its enantiomer 
      S(+)-ketoprofen (dexketoprofen), were evaluated, firstly, by acute and chronic 
      toxicity tests using 3 representative model organisms (Vibrio fischeri, 
      Pseudokirchneriella subcapitata, and Ceriodaphnia dubia) and, secondly, by 
      evaluating the responses of biotransformation systems and multidrug 
      resistance-associated proteins (MRP1/MRP2) using the Poeciliopsis lucida 
      hepatocellular carcinoma 1 (PLHC-1) fish hepatic cell line. Toxicity data from 
      both acute and chronic dexketoprofen exposure indicated higher sensitivity 
      through inhibition of bioluminescence and algal growth and through increased 
      mortality/immobilization compared to racemic ketoprofen exposure. The growth 
      inhibition test showed that racemic ketoprofen and dexketoprofen exhibited 
      different effect concentration values (240.2 and 65.6 mug/L, respectively). 
      Furthermore, racemic ketoprofen and dexketoprofen did not exert cytotoxic effects 
      in PLHC-1 cells and produced compound-, time-, and concentration-specific 
      differential effects on cytochrome P450 1A (CYP1A) and glutathione S-transferase 
      levels. For CYP1A, the effects of racemic ketoprofen and dexketoprofen differed 
      at the transcriptional and catalytic levels. Exposure to racemic ketoprofen and 
      dexketoprofen modulated MRP1 and MRP2 mRNA levels, and these effects were also 
      dependent on compound, exposure time, and concentration of the individual drug. 
      The present study revealed for the first time the interactions between these 
      NSAIDs and key detoxification systems and different sensitivity to the racemic 
      mixture compared to its enantiomer. Environ Toxicol Chem 2018;37:201-212. (c) 2017 
      SETAC.
CI  - (c) 2017 SETAC.
FAU - Mennillo, Elvira
AU  - Mennillo E
AD  - Department of Veterinary Sciences, University of Pisa, San Piero a Grado, Italy.
FAU - Arukwe, Augustine
AU  - Arukwe A
AD  - Department of Biology, Norwegian University of Science and Technology (NTNU), 
      Trondheim, Norway.
FAU - Monni, Gianfranca
AU  - Monni G
AD  - Department of Veterinary Sciences, University of Pisa, San Piero a Grado, Italy.
FAU - Meucci, Valentina
AU  - Meucci V
AD  - Department of Veterinary Sciences, University of Pisa, San Piero a Grado, Italy.
FAU - Intorre, Luigi
AU  - Intorre L
AD  - Department of Veterinary Sciences, University of Pisa, San Piero a Grado, Italy.
FAU - Pretti, Carlo
AU  - Pretti C
AD  - Department of Veterinary Sciences, University of Pisa, San Piero a Grado, Italy.
AD  - Interuniversity Center of Marine Biology (CIBM) "G. Bacci," Livorno, Italy.
LA  - eng
PT  - Journal Article
DEP - 20171011
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Biomarkers)
RN  - 0 (Multidrug Resistance-Associated Proteins)
RN  - 0 (RNA, Messenger)
RN  - 90Y4QC304K (Ketoprofen)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Aliivibrio fischeri/physiology
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/toxicity
MH  - Biological Assay/*methods
MH  - Biomarkers/*metabolism
MH  - Biotransformation/drug effects
MH  - Cell Line
MH  - Cyprinodontiformes/growth & development/*metabolism
MH  - Cytochrome P-450 CYP1A1/genetics/metabolism
MH  - *Ecotoxicology
MH  - *Fresh Water
MH  - Glutathione Transferase/metabolism
MH  - Ketoprofen/*chemistry/*toxicity
MH  - Luminescence
MH  - Multidrug Resistance-Associated Proteins/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Reproduction/drug effects
MH  - Stereoisomerism
MH  - Survival Analysis
MH  - Toxicity Tests, Acute
MH  - Toxicity Tests, Chronic
OTO - NOTNLM
OT  - Bioassay
OT  - Biomarker
OT  - Ecotoxicology
OT  - Nonsteroidal anti-inflammatory drug
OT  - PLHC-1 cell
OT  - Pharmaceutical
EDAT- 2017/08/11 06:00
MHDA- 2018/10/23 06:00
CRDT- 2017/08/11 06:00
PHST- 2017/02/02 00:00 [received]
PHST- 2017/04/21 00:00 [revised]
PHST- 2017/08/08 00:00 [accepted]
PHST- 2017/08/11 06:00 [pubmed]
PHST- 2018/10/23 06:00 [medline]
PHST- 2017/08/11 06:00 [entrez]
AID - 10.1002/etc.3943 [doi]
PST - ppublish
SO  - Environ Toxicol Chem. 2018 Jan;37(1):201-212. doi: 10.1002/etc.3943. Epub 2017 
      Oct 11.