PMID- 28796894
OWN - NLM
STAT- MEDLINE
DCOM- 20180219
LR  - 20191210
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 65
IP  - 5
DP  - 2017 Nov
TI  - Subtractive phage display selection for screening and identification of peptide 
      sequences with potential use in serodiagnosis of paracoccidioidomycosis caused by 
      Paracoccidioides brasiliensis.
PG  - 346-353
LID - 10.1111/lam.12788 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic granulomatous disease endemic in Latin 
      America whose aetiologic agents are the thermodimorphic fungi Paracoccidioides 
      brasiliensis and Paracoccidioides lutzii. Despite technological advances, some 
      problems have been reported for the fungal antigens used for serological 
      diagnosis, and inconsistencies among laboratories have been reported. The use of 
      synthetic peptides in the serological diagnosis of infectious diseases has proved 
      to be a valuable strategy because in some cases, the reactions are more specific 
      and sensitive. In this study, we used a subtractive selection with a phage 
      display library against purified polyclonal antibodies for negative and positive 
      PCM sera caused by P. brasiliensis. The binding phages were sequenced and tested 
      in a binding assay to evaluate its interaction with sera from normal individuals 
      and PCM patients. Synthetic peptides derived from these phage clones were tested 
      in a serological assay, and we observed a significant recognition of LP15 by sera 
      from PCM patients infected with P. brasiliensis. Our results demonstrated that 
      subtractive phage display selection may be useful for identifying new epitopes 
      that can be applied to the serodiagnosis of PCM caused by P. brasiliensis. 
      SIGNIFICANCE AND IMPACT OF THE STUDY: Currently, there is no standardized method 
      for the preparation of paracoccidioidomycosis (PCM) antigens, which has resulted 
      in differences in the antigens used for serological diagnosis. Here, we report a 
      procedure that uses subtractive phage display selection to select and identify 
      new epitopes for the serodiagnosis of PCM caused by Paracoccidioides 
      brasiliensis. A synthetic peptide obtained using this methodology was 
      successfully recognized by sera from PCM patients, thus demonstrating its 
      potential use for improving the serodiagnosis of this mycosis. The development of 
      synthetic peptides for the serodiagnosis of PCM could be a promising alternative 
      for the better standardization of diagnoses among laboratories.
CI  - (c) 2017 The Society for Applied Microbiology.
FAU - Portes, L da Silva
AU  - Portes LDS
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Sao Paulo, Brasil.
FAU - Kioshima, E S
AU  - Kioshima ES
AD  - Laboratorio de Micologia Medica, Departamento de Analises Clinicas, Universidade 
      Estadual de Maringa, Maringa, Brasil.
FAU - de Camargo, Z P
AU  - de Camargo ZP
AD  - Laboratorio de Micologia Medica e Molecular, Departamento de Microbiologia, 
      Imunologia e Parasitologia, Universidade Federal de Sao Paulo - Campus Sao Paulo, 
      Sao Paulo, Brasil.
FAU - Batista, W L
AU  - Batista WL
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Sao Paulo, Brasil.
FAU - Xander, P
AU  - Xander P
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Sao Paulo, Brasil.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20170921
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Peptide Library)
RN  - 0 (Peptides)
SB  - IM
MH  - Antibodies, Fungal/blood
MH  - Antigens, Fungal/blood/immunology
MH  - Bacteriophages/genetics/metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Paracoccidioides/genetics/immunology/*isolation & purification
MH  - Paracoccidioidomycosis/blood/*diagnosis/microbiology
MH  - Peptide Library
MH  - Peptides/genetics/immunology
MH  - Serologic Tests/instrumentation/*methods
OTO - NOTNLM
OT  - ELISA
OT  - paracoccidioidomycosis
OT  - phage display
OT  - serodiagnosis
OT  - synthetic peptides
EDAT- 2017/08/11 06:00
MHDA- 2018/02/20 06:00
CRDT- 2017/08/11 06:00
PHST- 2017/04/05 00:00 [received]
PHST- 2017/08/01 00:00 [revised]
PHST- 2017/08/01 00:00 [accepted]
PHST- 2017/08/11 06:00 [pubmed]
PHST- 2018/02/20 06:00 [medline]
PHST- 2017/08/11 06:00 [entrez]
AID - 10.1111/lam.12788 [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2017 Nov;65(5):346-353. doi: 10.1111/lam.12788. Epub 2017 
      Sep 21.