PMID- 28797041 OWN - NLM STAT- MEDLINE DCOM- 20171009 LR - 20181113 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 12 IP - 8 DP - 2017 TI - Repeat low-level blast exposure increases transient receptor potential vanilloid 1 (TRPV1) and endothelin-1 (ET-1) expression in the trigeminal ganglion. PG - e0182102 LID - 10.1371/journal.pone.0182102 [doi] LID - e0182102 AB - Blast-associated sensory and cognitive trauma sustained by military service members is an area of extensively studied research. Recent studies in our laboratory have revealed that low-level blast exposure increased expression of transient receptor potential vanilloid 1 (TRPV1) and endothelin-1 (ET-1), proteins well characterized for their role in mediating pain transmission, in the cornea. Determining the functional consequences of these alterations in protein expression is critical to understanding blast-related sensory trauma. Thus, the purpose of this study was to examine TRPV1 and ET-1 expression in ocular associated sensory tissues following primary and tertiary blast. A rodent model of blast injury was used in which anesthetized animals, unrestrained or restrained, received a single or repeat blast (73.8 +/- 5.5 kPa) from a compressed air shock tube once or daily for five consecutive days, respectively. Behavioral and functional analyses were conducted to assess blast effects on nocifensive behavior and TRPV1 activity. Immunohistochemistry and Western Blot were also performed with trigeminal ganglia (TG) to determine TRPV1, ET-1 and glial fibrillary associated protein (GFAP) expression following blast. Increased TRPV1, ET-1 and GFAP were detected in the TG of animals exposed to repeat blast. Increased nocifensive responses were also observed in animals exposed to repeat, tertiary blast as compared to single blast and control. Moreover, decreased TRPV1 desensitization was observed in TG neurons exposed to repeat blast. Repeat, tertiary blast resulted in increased TRPV1, ET-1 and GFAP expression in the TG, enhanced nociception and decreased TRPV1 desensitization. FAU - Por, Elaine D AU - Por ED AUID- ORCID: 0000-0001-8025-5713 AD - Ocular Trauma, United States Army Institute of Surgical Research, Fort Sam, Houston, Texas, United States of America. FAU - Sandoval, Melody L AU - Sandoval ML AD - Ocular Trauma, United States Army Institute of Surgical Research, Fort Sam, Houston, Texas, United States of America. FAU - Thomas-Benson, Chiquita AU - Thomas-Benson C AD - Ocular Trauma, United States Army Institute of Surgical Research, Fort Sam, Houston, Texas, United States of America. FAU - Burke, Teresa A AU - Burke TA AD - Ocular Trauma, United States Army Institute of Surgical Research, Fort Sam, Houston, Texas, United States of America. FAU - Doyle Brackley, Allison AU - Doyle Brackley A AD - Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America. FAU - Jeske, Nathaniel A AU - Jeske NA AD - Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America. AD - Department of Oral and Maxillofacial Surgery, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America. AD - Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America. FAU - Cleland, Jeffery M AU - Cleland JM AD - Ocular Trauma, United States Army Institute of Surgical Research, Fort Sam, Houston, Texas, United States of America. FAU - Lund, Brian J AU - Lund BJ AD - Ocular Trauma, United States Army Institute of Surgical Research, Fort Sam, Houston, Texas, United States of America. LA - eng GR - F31 DE025551/DE/NIDCR NIH HHS/United States PT - Journal Article DEP - 20170810 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Endothelin-1) RN - 0 (TRPV Cation Channels) RN - 0 (Trpv1 protein, rat) SB - IM MH - Animals MH - Blast Injuries/*metabolism MH - Endothelin-1/*metabolism MH - Male MH - Neurons/*metabolism MH - Rats MH - Rats, Long-Evans MH - TRPV Cation Channels/*metabolism MH - Trigeminal Ganglion/*metabolism PMC - PMC5552217 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2017/08/11 06:00 MHDA- 2017/10/11 06:00 PMCR- 2017/08/10 CRDT- 2017/08/11 06:00 PHST- 2017/04/14 00:00 [received] PHST- 2017/07/12 00:00 [accepted] PHST- 2017/08/11 06:00 [entrez] PHST- 2017/08/11 06:00 [pubmed] PHST- 2017/10/11 06:00 [medline] PHST- 2017/08/10 00:00 [pmc-release] AID - PONE-D-17-14616 [pii] AID - 10.1371/journal.pone.0182102 [doi] PST - epublish SO - PLoS One. 2017 Aug 10;12(8):e0182102. doi: 10.1371/journal.pone.0182102. eCollection 2017.