PMID- 28798082
OWN - NLM
STAT- MEDLINE
DCOM- 20180514
LR  - 20201209
IS  - 1477-9145 (Electronic)
IS  - 0022-0949 (Linking)
VI  - 220
IP  - Pt 20
DP  - 2017 Oct 15
TI  - Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid 
      herpesvirus 1 replication.
PG  - 3671-3685
LID - 10.1242/jeb.156299 [doi]
AB  - Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used 
      reverse genetic tool for determining the loss-of-function phenotype of a gene. 
      Here, the possible induction of an immune response by long dsRNA was tested in a 
      marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 
      of the nuclear factor kappaB inhibitor (IkappaB2). This gene is a candidate of particular 
      interest for functional investigations in the context of oyster mass mortality 
      events, as Cg-IkappaB2 mRNA levels exhibited significant variation depending on the 
      amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, 
      dsRNAs targeting Cg-IkappaB2 and green fluorescent protein genes were injected in 
      vivo into oysters before being challenged by OsHV-1. Survival appeared close to 
      100% in both dsRNA-injected conditions associated with a low detection of viral 
      DNA and a low expression of a panel of 39 OsHV-1 genes as compared with infected 
      control. Long dsRNA molecules, both Cg-IkappaB2- and GFP-dsRNA, may have induced an 
      anti-viral state controlling the OsHV-1 replication and precluding the 
      understanding of the specific role of Cg-IkappaB2 Immune-related genes including 
      Cg-IkappaB1, Cg-Rel1, Cg-IFI44, Cg-PKR and Cg-IAP appeared activated in the 
      dsRNA-injected condition, potentially hampering viral replication and thus 
      conferring a better resistance to OsHV-1 infection. We revealed that long 
      dsRNA-mediated genetic interference triggered an anti-viral state in the oyster, 
      emphasizing the need for new reverse genetics tools for assessing immune gene 
      function and avoiding off-target effects in bivalves.
CI  - (c) 2017. Published by The Company of Biologists Ltd.
FAU - Pauletto, Marianna
AU  - Pauletto M
AUID- ORCID: 0000-0002-2424-7484
AD  - Department of Comparative Biomedicine and Food Science, University of Padova, 
      Viale dell'Universita 16, 35020 Legnaro (PD), Italy marianna.pauletto@unipd.it.
FAU - Segarra, Amelie
AU  - Segarra A
AD  - Ifremer, Laboratoire de Genetique et Pathologie des Mollusques Marins, 17390 La 
      Tremblade, France.
FAU - Montagnani, Caroline
AU  - Montagnani C
AD  - Ifremer, IHPE UMR 5244, Univ. Perpignan Via Domitia, CNRS, Univ. Montpellier, 
      F-34095, Montpellier, France.
FAU - Quillien, Virgile
AU  - Quillien V
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, LEMAR, 29280 Plouzane, France.
FAU - Faury, Nicole
AU  - Faury N
AD  - Ifremer, Laboratoire de Genetique et Pathologie des Mollusques Marins, 17390 La 
      Tremblade, France.
FAU - Le Grand, Jacqueline
AU  - Le Grand J
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, LEMAR, 29280 Plouzane, France.
FAU - Miner, Philippe
AU  - Miner P
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, LEMAR, 29280 Plouzane, France.
FAU - Petton, Bruno
AU  - Petton B
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, LEMAR, 29280 Plouzane, France.
FAU - Labreuche, Yannick
AU  - Labreuche Y
AD  - Sorbonne Universites, UPMC Paris 06, CNRS, UMR 8227, Station Biologique de 
      Roscoff, CS 90074, F-29688 Roscoff cedex, France.
FAU - Fleury, Elodie
AU  - Fleury E
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, LEMAR, 29280 Plouzane, France.
FAU - Fabioux, Caroline
AU  - Fabioux C
AD  - Institut Universitaire Europeen de la Mer, Universite de Bretagne Occidentale, 
      UMR 6539 CNRS/UBO/IRD/Ifremer, LEMAR, 29280 Plouzane, France.
FAU - Bargelloni, Luca
AU  - Bargelloni L
AD  - Department of Comparative Biomedicine and Food Science, University of Padova, 
      Viale dell'Universita 16, 35020 Legnaro (PD), Italy.
FAU - Renault, Tristan
AU  - Renault T
AD  - Ifremer, Departement Ressources Biologiques et Environnement, rue de l'Ile d'Yeu, 
      44000 Nantes, France.
FAU - Huvet, Arnaud
AU  - Huvet A
AD  - Ifremer, UMR 6539 CNRS/UBO/IRD/Ifremer, LEMAR, 29280 Plouzane, France.
LA  - eng
PT  - Journal Article
DEP - 20170810
PL  - England
TA  - J Exp Biol
JT  - The Journal of experimental biology
JID - 0243705
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Double-Stranded)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - Crassostrea/*genetics/*immunology
MH  - DNA Viruses/*physiology
MH  - DNA, Viral/genetics
MH  - *Immunity, Innate
MH  - RNA, Double-Stranded/*genetics/metabolism
OTO - NOTNLM
OT  - Anti-viral response
OT  - Inhibitor of NF-kappaB
OT  - Marine bivalve
OT  - Ostreid herpesvirus 1
OT  - RNA interference
COIS- Competing interestsThe authors declare no competing or financial interests.
EDAT- 2017/08/12 06:00
MHDA- 2018/05/15 06:00
CRDT- 2017/08/12 06:00
PHST- 2017/01/12 00:00 [received]
PHST- 2017/08/07 00:00 [accepted]
PHST- 2017/08/12 06:00 [pubmed]
PHST- 2018/05/15 06:00 [medline]
PHST- 2017/08/12 06:00 [entrez]
AID - jeb.156299 [pii]
AID - 10.1242/jeb.156299 [doi]
PST - ppublish
SO  - J Exp Biol. 2017 Oct 15;220(Pt 20):3671-3685. doi: 10.1242/jeb.156299. Epub 2017 
      Aug 10.