PMID- 28799001
OWN - NLM
STAT- MEDLINE
DCOM- 20180430
LR  - 20180710
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 409
IP  - 26
DP  - 2017 Oct
TI  - Switch-on fluorescent strategy based on crystal violet-functionalized CdTe 
      quantum dots for detecting L-cysteine and glutathione in water and urine.
PG  - 6081-6090
LID - 10.1007/s00216-017-0541-1 [doi]
AB  - The concentration of L-cysteine (Cys) and glutathione (GSH) is closely related to 
      the critical risk of various diseases. In our study, a new rapid method for the 
      determination of Cys and GSH in water and urine samples has been developed using 
      a fluorescent probe technique, which was based on crystal violet 
      (CV)-functionalized CdTe quantum dots (QDs). The original QDs emitted 
      fluorescence light, which was turned off upon adding CV. This conjugation of CV 
      and QDs could be attributed to electrostatic interaction between COO(-) of 
      mercaptopropionic acid (MPA) on the surface of QDs and N(+) of CV in aqueous 
      solution. In addition, Forster resonance energy transfer (FRET) also occurred 
      between CdTe QDs and CV. After adding Cys or GSH to the solution, Cys or GSH 
      exhibited a stronger binding preference toward Cd(2+) than Cd(2+)-MPA, which 
      disturbed the interaction between MPA and QDs. Thus, most MPA was able to be 
      separated from the surface of QDs because of the participation of Cys or GSH. 
      Then, the fluorescence intensity of the CdTe QDs was enhanced. Good linear 
      relationships were obtained in the range of 0.02-40 mug mL(-1) and 
      0.02-50 mug mL(-1), and the detection limits were calculated as 10.5 ng mL(-1) and 
      8.2 ng mL(-1), for Cys and GSH, respectively. In addition, the concentrations of 
      biological thiols in water and urine samples were determined by the standard 
      addition method using Cys as the standard; the quantitative recoveries were in 
      the range of 97.3-105.8%, and relative standard deviations (RSDs) ranged from 2.5 
      to 3.7%. The method had several unique properties, such as simplicity, lower 
      cost, high sensitivity, and environmental acceptability. Graphical abstract 
      Crystal violet-functionalized CdTe quantum dots for detecting L-cysteine and 
      glutathione with switch-on fluorescent strategy.
FAU - Sheng, Zhen
AU  - Sheng Z
AD  - Department of Chemistry, College of Science, Northeast Forestry University, 26 
      Hexing Road, Harbin, Heilongjiang, 150040, China.
FAU - Chen, Ligang
AU  - Chen L
AD  - Department of Chemistry, College of Science, Northeast Forestry University, 26 
      Hexing Road, Harbin, Heilongjiang, 150040, China. ligangchen2010@aliyun.com.
LA  - eng
GR  - No.2572017EB08/Fundamental Research Funds for the Central Universities/
GR  - 2016RAQXJ151/Harbin science and technology innovation talent research special 
      funds/
PT  - Journal Article
DEP - 20170810
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Cadmium Compounds)
RN  - 0 (Fluorescent Dyes)
RN  - 059QF0KO0R (Water)
RN  - GAN16C9B8O (Glutathione)
RN  - J4Z741D6O5 (Gentian Violet)
RN  - K848JZ4886 (Cysteine)
RN  - NQA0O090ZJ (Tellurium)
RN  - STG188WO13 (cadmium telluride)
SB  - IM
MH  - Cadmium Compounds/*chemistry
MH  - Cysteine/analysis/*urine
MH  - Fluorescent Dyes/*chemistry
MH  - Gentian Violet/*chemistry
MH  - Glutathione/analysis/*urine
MH  - Humans
MH  - Limit of Detection
MH  - Quantum Dots/*chemistry/ultrastructure
MH  - Spectrometry, Fluorescence/economics/methods
MH  - Tellurium/*chemistry
MH  - Water/*analysis
OTO - NOTNLM
OT  - Fluorescent
OT  - Glutathione
OT  - L-Cysteine
OT  - Quantum dot
OT  - Switch-on
EDAT- 2017/08/12 06:00
MHDA- 2018/05/01 06:00
CRDT- 2017/08/12 06:00
PHST- 2017/05/22 00:00 [received]
PHST- 2017/07/20 00:00 [accepted]
PHST- 2017/06/26 00:00 [revised]
PHST- 2017/08/12 06:00 [pubmed]
PHST- 2018/05/01 06:00 [medline]
PHST- 2017/08/12 06:00 [entrez]
AID - 10.1007/s00216-017-0541-1 [pii]
AID - 10.1007/s00216-017-0541-1 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2017 Oct;409(26):6081-6090. doi: 10.1007/s00216-017-0541-1. 
      Epub 2017 Aug 10.