PMID- 28801811 OWN - NLM STAT- MEDLINE DCOM- 20181010 LR - 20181113 IS - 1434-9949 (Electronic) IS - 0770-3198 (Linking) VI - 36 IP - 10 DP - 2017 Oct TI - Association of variably expressed KIR3dl1 alleles with psoriatic disease. PG - 2261-2266 LID - 10.1007/s10067-017-3784-5 [doi] AB - The purpose of this study is to examine the genetic interaction of variably expressed killer cell immunoglobulin-like receptor (KIR) 3DL1 alleles with their cognate ligand, human leukocyte antigen (HLA)-Bw4, in susceptibility to psoriatic disease (PsD). A novel allelic typing system was developed to differentiate KIR3DL1 alleles (*High, *Low, *Null expression, and 3DS1), in PsD patients, including those with psoriatic arthritis (PsA) and cutaneous psoriasis without arthritis (PsC) and healthy controls. Frequencies of each KIR3DL1 allele, Bw4-80I and Bw4-80T, as well as the genetic interaction between the KIR3DL1 alleles and the Bw4 epitope were analyzed. KIR3DL1 alleles were successfully genotyped in 392 PsA, 260 PsC, and 371 control subjects. Only the KIR3DL1*Null allele was associated with PsD (OR = 0.69, p = 0.008), both in the PsA (OR = 0.69, p = 0.02) and PsC patients (OR = 0.70, p = 0.04) compared to control subjects. No difference in the frequency of KIR3DL1*Null was found between the PsA and PsC patients. The presence of the HLA-Bw4 epitope was significantly associated with PsD, particularly in the PsA patients compared to controls. Bw4-80I was increased in PsD and PsA subjects, but not in PsC patients compared to controls. Bw4-80T was increased in PsA compared to both PsC patients or to controls. No interaction was detected between any of the KIR3DL1 alleles and HLA-Bw4, Bw4-80I, or Bw4-80T. The novel qPCR technique successfully identified the four variably expressed KIR3DL1 alleles. The HLA-Bw4 epitope was associated with psoriatic disease, particularly with PsA, but no genetic interactions with KIR3DL1 alleles were detected. FAU - Berinstein, Jeffrey AU - Berinstein J AD - University of Toronto Psoriatic Arthritis Clinic, Centre for Prognosis Studies in the Rheumatic Diseases 1E-410B, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, M5T 2S8, Canada. AD - Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada. AD - Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA. FAU - Pollock, Remy AU - Pollock R AD - University of Toronto Psoriatic Arthritis Clinic, Centre for Prognosis Studies in the Rheumatic Diseases 1E-410B, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, M5T 2S8, Canada. AD - Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada. AD - Department of Medicine, Faculty of Medicine, University of Toronto, Toronto, ON, Canada. FAU - Pellett, Fawnda AU - Pellett F AD - University of Toronto Psoriatic Arthritis Clinic, Centre for Prognosis Studies in the Rheumatic Diseases 1E-410B, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, M5T 2S8, Canada. AD - Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada. FAU - Thavaneswaran, Arane AU - Thavaneswaran A AD - University of Toronto Psoriatic Arthritis Clinic, Centre for Prognosis Studies in the Rheumatic Diseases 1E-410B, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, M5T 2S8, Canada. AD - Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada. FAU - Chandran, Vinod AU - Chandran V AD - University of Toronto Psoriatic Arthritis Clinic, Centre for Prognosis Studies in the Rheumatic Diseases 1E-410B, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, M5T 2S8, Canada. AD - Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada. AD - Department of Medicine, Faculty of Medicine, University of Toronto, Toronto, ON, Canada. AD - Institute of Medical Science, University of Toronto, Toronto, ON, Canada. AD - Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada. FAU - Gladman, Dafna D AU - Gladman DD AUID- ORCID: 0000-0002-9074-0592 AD - University of Toronto Psoriatic Arthritis Clinic, Centre for Prognosis Studies in the Rheumatic Diseases 1E-410B, Toronto Western Hospital, 399 Bathurst Street, Toronto, ON, M5T 2S8, Canada. dafna.gladman@utoronto.ca. AD - Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada. dafna.gladman@utoronto.ca. AD - Department of Medicine, Faculty of Medicine, University of Toronto, Toronto, ON, Canada. dafna.gladman@utoronto.ca. AD - Institute of Medical Science, University of Toronto, Toronto, ON, Canada. dafna.gladman@utoronto.ca. LA - eng PT - Journal Article DEP - 20170811 PL - Germany TA - Clin Rheumatol JT - Clinical rheumatology JID - 8211469 RN - 0 (Epitopes) RN - 0 (HLA Antigens) RN - 0 (HLA-B Antigens) RN - 0 (HLA-Bw4 antigen) RN - 0 (KIR3DL1 protein, human) RN - 0 (Receptors, KIR3DL1) SB - IM MH - Adult MH - Alleles MH - Arthritis, Psoriatic/*genetics MH - Case-Control Studies MH - Epitopes/chemistry MH - Female MH - *Genetic Predisposition to Disease MH - Genotype MH - HLA Antigens/genetics MH - HLA-B Antigens/genetics MH - Humans MH - Inflammation MH - Male MH - Middle Aged MH - Polymerase Chain Reaction MH - Receptors, KIR3DL1/*genetics OTO - NOTNLM OT - KIR OT - KIR3DL1 OT - Psoriasis OT - Psoriatic arthritis OT - Psoriatic disease EDAT- 2017/08/13 06:00 MHDA- 2018/10/12 06:00 CRDT- 2017/08/13 06:00 PHST- 2017/06/12 00:00 [received] PHST- 2017/07/31 00:00 [accepted] PHST- 2017/07/26 00:00 [revised] PHST- 2017/08/13 06:00 [pubmed] PHST- 2018/10/12 06:00 [medline] PHST- 2017/08/13 06:00 [entrez] AID - 10.1007/s10067-017-3784-5 [pii] AID - 10.1007/s10067-017-3784-5 [doi] PST - ppublish SO - Clin Rheumatol. 2017 Oct;36(10):2261-2266. doi: 10.1007/s10067-017-3784-5. Epub 2017 Aug 11.