PMID- 28804503
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2017
DP  - 2017
TI  - Effects of Angelica Extract on Schwann Cell Proliferation and Expressions of 
      Related Proteins.
PG  - 6358392
LID - 10.1155/2017/6358392 [doi]
LID - 6358392
AB  - The present study investigated the effects of Angelica extract (AE) on Schwann 
      cell proliferation and expressions of related proteins, including brain derived 
      neurotrophic factor (BDNF), neural cell adhesion molecule (NCAM), and 
      proliferating cell nuclear antigen (PCNA). Proliferation activity and cell cycles 
      of SCs were evaluated by MTT assay and flow cytometry methods, respectively, 
      after 12 h treatment of AE at different concentrations (62.5, 125, 250, 1000, 
      2000, 4000, and 8000 mg/L). SCs were treated by 500, 1000, and 2000 mg/L AE for 
      24 h or 48 h; the related genes mRNA and proteins expressions in SCs were 
      detected by quantitative real-time reverse transcription-polymerase chain 
      reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) kit. At the 
      concentration range of 125-2000 mg/L, the SC proliferation was induced by AE in a 
      dose-dependent manner, especially 1000 and 2000 mg/L; cells in drug-treated 
      groups showed the most increase. Cells counts were ascended significantly in 
      (G2/M + S) phase compared to control group. BDNF, NCAM, and PCNA protein 
      expressions significantly increased at drug-treated groups. Relative genes mRNA 
      expressions levels were also significantly higher compared to control group. The 
      results indicated that AE facilitated SC proliferation and related genes and 
      proteins expressions, which provided a basic guideline for nerve injury repair in 
      clinic.
FAU - Jiang, Xiaowen
AU  - Jiang X
AUID- ORCID: 0000-0002-4515-6266
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Liu, Lin
AU  - Liu L
AUID- ORCID: 0000-0001-7774-2537
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Zhang, Binqing
AU  - Zhang B
AUID- ORCID: 0000-0002-0655-136X
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Lu, Ziyin
AU  - Lu Z
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Qiao, Lu
AU  - Qiao L
AUID- ORCID: 0000-0002-5760-3975
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Feng, Xinxin
AU  - Feng X
AUID- ORCID: 0000-0002-1133-0422
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      China.
FAU - Yu, Wenhui
AU  - Yu W
AUID- ORCID: 0000-0002-3744-4575
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 
      China.
AD  - Key Laboratory of the Provincial Education, Department of Heilongjiang for Common 
      Animal Disease Prevention and Treatment, Northeast Agricultural University, 
      Harbin 150030, China.
LA  - eng
PT  - Journal Article
DEP - 20170718
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC5540469
EDAT- 2017/08/15 06:00
MHDA- 2017/08/15 06:01
PMCR- 2017/07/18
CRDT- 2017/08/15 06:00
PHST- 2017/03/12 00:00 [received]
PHST- 2017/05/25 00:00 [accepted]
PHST- 2017/08/15 06:00 [entrez]
PHST- 2017/08/15 06:00 [pubmed]
PHST- 2017/08/15 06:01 [medline]
PHST- 2017/07/18 00:00 [pmc-release]
AID - 10.1155/2017/6358392 [doi]
PST - ppublish
SO  - Evid Based Complement Alternat Med. 2017;2017:6358392. doi: 10.1155/2017/6358392. 
      Epub 2017 Jul 18.