PMID- 28805916
OWN - NLM
STAT- MEDLINE
DCOM- 20180125
LR  - 20231213
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 95
IP  - 8
DP  - 2017 Aug
TI  - The reduction of starch in finishing diets supplemented with oil does not prevent 
      the accumulation of trans-10 18:1 in lamb meat.
PG  - 3745-3761
LID - 10.2527/jas.2017.1578 [doi]
AB  - The experiment was conducted to test the hypothesis that the replacement of 
      cereal with low-starch feed ingredients in lambs' finishing diets supplemented 
      with oils could prevent the accumulation of -10-18:1 in meat. Forty lambs were 
      fed 1 of 4 diets supplemented with soybean oil (5.9%) and fish oil (1%) for 6 wk 
      before slaughter. The control (CON) diet contained 43% barley, and in the other 
      diets, barley was completely replaced by dehydrated citrus pulp (DCP), dehydrated 
      sugar beet pulp (DBP), or soybean hulls (SH). Growth performance, feed intake, 
      and carcass and meat quality traits were analyzed. At slaughter, LM samples were 
      collected for gene expression evaluation, and 3 d after slaughter, LM and 
      subcutaneous (s.c.) fat samples were collected for fatty acid analysis. None of 
      the diets affected meat quality, but the DCP diet reduced ADG ( < 0.05) and the 
      DCP and SH diets decreased the feed-to-gain ratio ( < 0.01). The DCP diet 
      increased ( < 0.05) the risk of parakeratosis and the severity of the lesions. 
      Moreover, the DBP treatment led to increased a* (redness) and b* (yellowness) in 
      s.c. fat compared with the CON treatment ( < 0.05). The lipid content of LM did 
      not differ ( > 0.05) with treatment and averaged 34.4 g/kg of meat. Diets had no 
      effect ( > 0.05) on SFA, PUFA, and -MUFA sums and on the -6:-3 ratio in both LM 
      and s.c. fat. A lower expression of fatty acid synthase (FASN) was found with the 
      DCP treatment than with the other treatments ( < 0.001). All treatments showed a 
      high accumulation of -10-18:1, averaging 91 mg/g fatty acid in LM and 147 mg/g 
      fatty acid in s.c. fat. The concentration of -11-18:1 in the tissues was 
      considerably lower than that of -10-18:1, and thus the -10-18:1:-11-18:1 ratio 
      was above 3 with all treatments. Despite this, the SH diet clearly promoted a 
      larger deposition of -11-18:1 and -9,-11-18:2 in tissues compared with the other 
      treatments. () gene expression and SCD activity index in LM were reduced with the 
      SH diet compared with the CON and DCP diets. Overall, these results clearly 
      showed, for the first time, that low-starch/high-NDF diets are not able to 
      prevent the establishment of -10 shifted rumen biohydrogenation pathways, 
      evaluated by the deposition of biohydrogenation intermediates in lamb meat and 
      fat.
FAU - Costa, M
AU  - Costa M
FAU - Alves, S P
AU  - Alves SP
FAU - Francisco, A
AU  - Francisco A
FAU - Almeida, J
AU  - Almeida J
FAU - Alfaia, C M
AU  - Alfaia CM
FAU - Martins, S V
AU  - Martins SV
FAU - Prates, J A M
AU  - Prates JAM
FAU - Santos-Silva, J
AU  - Santos-Silva J
FAU - Doran, O
AU  - Doran O
FAU - Bessa, R J B
AU  - Bessa RJB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Fatty Acids)
RN  - 8001-22-7 (Soybean Oil)
RN  - 9005-25-8 (Starch)
SB  - IM
MH  - Animal Feed/analysis
MH  - Animals
MH  - Diet/veterinary
MH  - *Dietary Supplements
MH  - Fatty Acids/*metabolism
MH  - Hydrogenation
MH  - Male
MH  - Random Allocation
MH  - Red Meat/analysis/*standards
MH  - Rumen/metabolism
MH  - Sheep/*physiology
MH  - Soybean Oil/*pharmacology
MH  - Glycine max
MH  - Starch/*administration & dosage
MH  - Subcutaneous Fat/anatomy & histology
EDAT- 2017/08/15 06:00
MHDA- 2018/01/26 06:00
CRDT- 2017/08/15 06:00
PHST- 2017/08/15 06:00 [entrez]
PHST- 2017/08/15 06:00 [pubmed]
PHST- 2018/01/26 06:00 [medline]
AID - 10.2527/jas.2017.1578 [doi]
PST - ppublish
SO  - J Anim Sci. 2017 Aug;95(8):3745-3761. doi: 10.2527/jas.2017.1578.