PMID- 28806534
OWN - NLM
STAT- MEDLINE
DCOM- 20171227
LR  - 20180319
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 95
IP  - 6
DP  - 2017 Dec
TI  - Knockdown of Snail inhibits epithelial-mesenchymal transition of human laryngeal 
      squamous cell carcinoma Hep-2 cells through the vitamin D receptor signaling 
      pathway.
PG  - 672-678
LID - 10.1139/bcb-2017-0039 [doi]
AB  - It has been well documented that Snail plays a decisive role in various tumors. 
      However, the direct effect of Snail on laryngeal squamous cell carcinoma (LSCC) 
      has not been elaborated. In this study, we firstly detected the expression of 
      Snail in 14 samples of patients with LSCC and found that its content was high in 
      cancer tissues compared with adjacent tissues. Then we established LSCC Hep-2 
      cells with Snail silencing and validated the knockdown efficiency by Western 
      blotting and real-time PCR. Results showed that silencing of Snail significantly 
      inhibited the ability of adhesion, migration, and invasion of Hep-2 cells. 
      Further study revealed that knockdown of Snail suppressed the 
      epithelial-mesenchymal transition (EMT) process of Hep-2 cells, as evidenced by 
      downregulation of matrix metallopeptidase (MMP)-2, MMP-9, integrin subunit beta 1 
      (ITGbeta1), beta-catenin, vimentin, N-cadherin, and fibronectin and upregulation of 
      vitamin D receptor (VDR) and E-cadherin. Additionally, transfection with the 
      small interfering RNA of VDR reversed the effect induced by Snail silencing in 
      Hep-2 cells. Taken together, these results demonstrate that knockdown of Snail 
      can inhibit the EMT process of LSCC cells through the VDR signaling pathway in 
      vitro.
FAU - Zhao, Xue
AU  - Zhao X
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
FAU - Yu, Dan
AU  - Yu D
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
FAU - Yang, Jingpu
AU  - Yang J
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
FAU - Xue, Kai
AU  - Xue K
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
FAU - Liu, Yan
AU  - Liu Y
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
FAU - Jin, Chunshun
AU  - Jin C
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin 
      University, Changchun 130041, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170814
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Snail Family Transcription Factors)
SB  - IM
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - *Epithelial-Mesenchymal Transition
MH  - Gene Silencing
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Calcitriol/*metabolism
MH  - *Signal Transduction
MH  - Snail Family Transcription Factors/*genetics/metabolism
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - Snail
OT  - carcinome epidermoide du larynx
OT  - epithelial-mesenchymal transition
OT  - laryngeal squamous cell carcinoma
OT  - recepteur de la vitamine D
OT  - transition epithelio-mesenchymateuse
OT  - vitamin D receptor
EDAT- 2017/08/15 06:00
MHDA- 2017/12/28 06:00
CRDT- 2017/08/15 06:00
PHST- 2017/08/15 06:00 [pubmed]
PHST- 2017/12/28 06:00 [medline]
PHST- 2017/08/15 06:00 [entrez]
AID - 10.1139/bcb-2017-0039 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2017 Dec;95(6):672-678. doi: 10.1139/bcb-2017-0039. Epub 2017 
      Aug 14.