PMID- 28806788
OWN - NLM
STAT- MEDLINE
DCOM- 20171009
LR  - 20181202
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 8
DP  - 2017
TI  - Chronic unpredictable stress exacerbates surgery-induced sickness behavior and 
      neuroinflammatory responses via glucocorticoids secretion in adult rats.
PG  - e0183077
LID - 10.1371/journal.pone.0183077 [doi]
LID - e0183077
AB  - METHODS: Sprague-Dawley adult male rats (12-14 weeks old) were exposed to 14-day 
      CUS and then subjected to partial hepatectomy 24 h after the last stress session. 
      The rats were pretreated with an antagonist of the glucocorticoids (GCs) receptor 
      RU486 (30 mg/kg, i.p.) 1 h prior to stress exposure. The behavioral changes were 
      evaluated with open field test and elevated plus-maze test. The hippocampal 
      cytokines interleukin (IL)-1beta and IL-6 were measured on postoperative days 1, 3 
      and 7. Ionized calcium binding adaptor protein (Iba)-1, microglial M2 phenotype 
      marker Arg1, brain derived neurotrophic factor (BDNF) and CD200 were also 
      examined at each time point. RESULTS: CUS exacerbated surgery-induced sickness 
      behavior. Exposure to CUS alone failed to alter the levels of pro-inflammatory 
      cytokines in the brain. However, CUS exaggerated surgery-induced pro-inflammatory 
      cytokines expression (e.g. IL-1beta and IL-6) and upregulated the levels of Iba-1 on 
      postoperative days 1 and 3. An additional significant decreased BDNF, CD200 and a 
      lower level of Arg1 were also observed in the stressed rats following surgical 
      procedure. Pretreatment with RU486 blunted the potentiating effects of CUS on 
      surgery-induced sickness behavior and neuroinflammatory responses. CONCLUSION: 
      Chronic unpredictable stress enhanced surgery-induced sickness behavior and 
      neuroinflammatory responses. Stress-induced GCs played a pivotal role in 
      enhancing surgery-induced neuroinflammatory processes by modulation of microglia 
      functions.
FAU - Wang, Na
AU  - Wang N
AD  - Department of Anesthesiology, The First Hospital of China Medical University, 
      Shenyang, Liaoning, China.
FAU - Ma, Hong
AU  - Ma H
AD  - Department of Anesthesiology, The First Hospital of China Medical University, 
      Shenyang, Liaoning, China.
FAU - Li, Zhe
AU  - Li Z
AD  - Department of Anesthesiology, The First Hospital of China Medical University, 
      Shenyang, Liaoning, China.
FAU - Gao, Yalei
AU  - Gao Y
AD  - Department of Anesthesiology, The First Hospital of China Medical University, 
      Shenyang, Liaoning, China.
FAU - Cao, Xuezhao
AU  - Cao X
AUID- ORCID: 0000-0003-4824-6934
AD  - Department of Anesthesiology, The First Hospital of China Medical University, 
      Shenyang, Liaoning, China.
FAU - Jiang, Yanhua
AU  - Jiang Y
AD  - Department of Anesthesiology, The First Hospital of China Medical University, 
      Shenyang, Liaoning, China.
FAU - Zhou, Yongjian
AU  - Zhou Y
AD  - Department of Anesthesiology, The First Hospital of China Medical University, 
      Shenyang, Liaoning, China.
FAU - Liu, Sidan
AU  - Liu S
AD  - Department of Anesthesiology, The First Hospital of China Medical University, 
      Shenyang, Liaoning, China.
LA  - eng
PT  - Journal Article
DEP - 20170814
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Aif1 protein, rat)
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Glucocorticoids)
RN  - 0 (Interleukin-6)
RN  - 0 (Microfilament Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.5.3.1 (Arginase)
RN  - UQ4V77A8VA (antigens, CD200)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/metabolism
MH  - Anxiety/blood/complications/physiopathology
MH  - Arginase/metabolism
MH  - Biomarkers/metabolism
MH  - Body Weight
MH  - Calcium-Binding Proteins/metabolism
MH  - Chronic Disease
MH  - Glucocorticoids/blood/*metabolism
MH  - Hepatectomy/*adverse effects
MH  - Hippocampus/pathology/physiopathology
MH  - *Illness Behavior
MH  - Inflammation/blood/*complications/pathology/physiopathology
MH  - Interleukin-6/metabolism
MH  - Male
MH  - Microfilament Proteins/metabolism
MH  - Microglia/metabolism
MH  - Motor Activity
MH  - Neurons/*pathology
MH  - Phenotype
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats, Sprague-Dawley
MH  - Stress, Psychological/blood/*complications/physiopathology
MH  - Up-Regulation
PMC - PMC5555668
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/08/15 06:00
MHDA- 2017/10/11 06:00
PMCR- 2017/08/14
CRDT- 2017/08/15 06:00
PHST- 2017/01/17 00:00 [received]
PHST- 2017/07/28 00:00 [accepted]
PHST- 2017/08/15 06:00 [entrez]
PHST- 2017/08/15 06:00 [pubmed]
PHST- 2017/10/11 06:00 [medline]
PHST- 2017/08/14 00:00 [pmc-release]
AID - PONE-D-17-02136 [pii]
AID - 10.1371/journal.pone.0183077 [doi]
PST - epublish
SO  - PLoS One. 2017 Aug 14;12(8):e0183077. doi: 10.1371/journal.pone.0183077. 
      eCollection 2017.