PMID- 28808100
OWN - NLM
STAT- MEDLINE
DCOM- 20180404
LR  - 20220129
IS  - 1471-2970 (Electronic)
IS  - 0962-8436 (Print)
IS  - 0962-8436 (Linking)
VI  - 372
IP  - 1730
DP  - 2017 Sep 26
TI  - Hacking the thylakoid proton motive force for improved photosynthesis: modulating 
      ion flux rates that control proton motive force partitioning into Deltapsi and DeltapH.
LID - 10.1098/rstb.2016.0381 [doi]
LID - 20160381
AB  - There is considerable interest in improving plant productivity by altering the 
      dynamic responses of photosynthesis in tune with natural conditions. This is 
      exemplified by the 'energy-dependent' form of non-photochemical quenching (q(E)), 
      the formation and decay of which can be considerably slower than natural light 
      fluctuations, limiting photochemical yield. In addition, we recently reported 
      that rapidly fluctuating light can produce field recombination-induced 
      photodamage (FRIP), where large spikes in electric field across the thylakoid 
      membrane (Deltapsi) induce photosystem II recombination reactions that produce damaging 
      singlet oxygen ((1)O(2)). Both q(E) and FRIP are directly linked to the thylakoid 
      proton motive force (pmf), and in particular, the slow kinetics of partitioning 
      pmf into its DeltapH and Deltapsi components. Using a series of computational simulations, 
      we explored the possibility of 'hacking' pmf partitioning as a target for 
      improving photosynthesis. Under a range of illumination conditions, increasing 
      the rate of counter-ion fluxes across the thylakoid membrane should lead to more 
      rapid dissipation of Deltapsi and formation of DeltapH. This would result in increased 
      rates for the formation and decay of q(E) while resulting in a more rapid decline 
      in the amplitudes of Deltapsi-spikes and decreasing (1)O(2) production. These results 
      suggest that ion fluxes may be a viable target for plant breeding or engineering. 
      However, these changes also induce transient, but substantial mismatches in the 
      ATP : NADPH output ratio as well as in the osmotic balance between the lumen and 
      stroma, either of which may explain why evolution has not already accelerated 
      thylakoid ion fluxes. Overall, though the model is simplified, it recapitulates 
      many of the responses seen in vivo, while spotlighting critical aspects of the 
      complex interactions between pmf components and photosynthetic processes. By 
      making the programme available, we hope to enable the community of photosynthesis 
      researchers to further explore and test specific hypotheses.This article is part 
      of the themed issue 'Enhancing photosynthesis in crop plants: targets for 
      improvement'.
CI  - (c) 2017 The Author(s).
FAU - Davis, Geoffry A
AU  - Davis GA
AD  - Department of Energy Plant Research Laboratory, Michigan State University, East 
      Lansing, MI 48824, USA.
AD  - Cell and Molecular Biology Graduate Program, Michigan State University, East 
      Lansing, MI 48824, USA.
FAU - Rutherford, A William
AU  - Rutherford AW
AD  - Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
FAU - Kramer, David M
AU  - Kramer DM
AUID- ORCID: 0000-0003-2181-6888
AD  - Department of Energy Plant Research Laboratory, Michigan State University, East 
      Lansing, MI 48824, USA kramerd8@cns.msu.edu.
AD  - Department of Biochemistry and Molecular Biology, Michigan State University, East 
      Lansing, MI 48824, USA.
LA  - eng
GR  - BB/K002627/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Review
PL  - England
TA  - Philos Trans R Soc Lond B Biol Sci
JT  - Philosophical transactions of the Royal Society of London. Series B, Biological 
      sciences
JID - 7503623
SB  - IM
MH  - *Photosynthesis
MH  - Plants/*metabolism
MH  - *Proton-Motive Force
MH  - Thylakoids/*metabolism
PMC - PMC5566881
OTO - NOTNLM
OT  - ATP synthase
OT  - bioenergetics
OT  - non-photochemical quenching
OT  - photoinhibition
OT  - photosynthesis
OT  - proton motive force
COIS- We have no competing interests.
EDAT- 2017/08/16 06:00
MHDA- 2018/04/05 06:00
PMCR- 2018/09/26
CRDT- 2017/08/16 06:00
PHST- 2017/05/31 00:00 [accepted]
PHST- 2017/08/16 06:00 [entrez]
PHST- 2017/08/16 06:00 [pubmed]
PHST- 2018/04/05 06:00 [medline]
PHST- 2018/09/26 00:00 [pmc-release]
AID - rstb.2016.0381 [pii]
AID - rstb20160381 [pii]
AID - 10.1098/rstb.2016.0381 [doi]
PST - ppublish
SO  - Philos Trans R Soc Lond B Biol Sci. 2017 Sep 26;372(1730):20160381. doi: 
      10.1098/rstb.2016.0381.