PMID- 28809762
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1523-7834 (Print)
IS  - 1523-7834 (Linking)
VI  - 43
IP  - 3
DP  - 2017
TI  - Elucidation of Novel Chromosomal Abnormalities in Pancreatic Cancer: Conventional 
      and Molecular Cytogenetic Characterization of 16 Pancreatic Cell Lines.
PG  - 113-127
AB  - Pancreatic carcinoma is a major cause of cancer-related death in the United 
      States, with a five-year survival rate of approximately 5%. Cytogenetic analysis 
      has identified clinically significant chromosomal abnormalities in numerous 
      malignancies, but it is not utilized in the clinical management of pancreatic 
      carcinoma. We performed conventional and molecular cytogenetic analysis of 16 
      pancreatic carcinoma cell lines using Giemsa banding and DNA-based fluorescence 
      in situ hybridization (FISH). Conventional cytogenetic analysis revealed a 
      diversity of recurrent and clonal numerical and structural abnormalities in all 
      cell lines analyzed, many of which occurred at loci of genes implicated in 
      pancreatic or related cancers. FISH analysis revealed significant decreases in 
      copy number of numerous tumor-suppressor genes including TP53, CDKN2A, and SMAD4. 
      In some cell lines, amplification of oncogenes HER2 and MYC was also observed. 
      Finally, novel rearrangements involving ARID1A and TGFBR2 were identified in a 
      small subset of cell lines by means of molecular cytogenetic analysis. All in 
      all, these data provide additional insight into recurrent chromosomal 
      abnormalities in pancreatic carcinoma that can potentially be utilized as 
      biomarkers in the clinical management of the disease. Investigation of other 
      aberrations as well as correlation of recurrent ones with clinicopathologic 
      features is warranted in order to assess the utility of cytogenetic analysis of 
      pancreatic carcinoma.
FAU - Shabsovich, David
AU  - Shabsovich D
AD  - The International Circle of Genetic Studies, Los Angeles, CA and University of 
      California - Los Angeles, Los Angeles, CA.
FAU - Tirado, Carlos A
AU  - Tirado CA
AD  - The International Circle of Genetic Studies, Los Angeles, CA and Allina Health, 
      Minneapolis, MN, HPS, Minneapolis, MN, and The University of Minnesota, School of 
      Medicine. Department of Laboratory Medicine and Pathology, Minneapolis, MN.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Assoc Genet Technol
JT  - Journal of the Association of Genetic Technologists
JID - 9807282
EDAT- 2017/08/16 06:00
MHDA- 2017/08/16 06:01
CRDT- 2017/08/16 06:00
PHST- 2017/08/16 06:00 [entrez]
PHST- 2017/08/16 06:00 [pubmed]
PHST- 2017/08/16 06:01 [medline]
PST - ppublish
SO  - J Assoc Genet Technol. 2017;43(3):113-127.