PMID- 28810199
OWN - NLM
STAT- MEDLINE
DCOM- 20180216
LR  - 20181202
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 231
IP  - Pt 1
DP  - 2017 Dec
TI  - Diallyl trisulfide (DATS) suppresses benzene-induced cytopenia by modulating 
      haematopoietic cell apoptosis.
PG  - 301-310
LID - S0269-7491(16)31092-2 [pii]
LID - 10.1016/j.envpol.2017.07.069 [doi]
AB  - Benzene is a well-known occupational and environmental toxicant associated with 
      cytopenia, which is characterized by a disorder in the peripheral blood cell 
      counts. However, no effective preventive strategy has been developed yet to 
      tackle the exposure to benzene in daily life. The aim of this study was to 
      evaluate the protective effects of diallyl trisulfide (DATS) on benzene-induced 
      haematopoietic damage and to reveal its potential mechanisms of action. In our 
      study, male Sprague-Dawley rats were divided into six groups. Rats were 
      administered with benzene (1.3 g/kg BW by gavage) to establish the benzene 
      poisoning model, while the DATS treatment groups were treated with benzene plus 
      DATS (15 mg/kg, 30 mg/kg, 45 mg/kg, respectively, by gavage) for 28 days. Our 
      results demonstrated that the counts of peripheral blood WBC and RBC decreased to 
      31.0% and 79.2%, respectively, in the benzene poisoning model group compared to 
      the control. However, blood cell counts were restored by DATS treatment 
      (30 mg/kg, 45 mg/kg). The apoptosis rates of peripheral blood mononuclear cells 
      (PBMCs) and bone marrow cells (BMCs) were increased to 274% and 284%, 
      respectively, following benzene exposure. Furthermore, expression levels of 
      Bcl-2, PI3K and p-Akt were downregulated and those of Bax were upregulated in 
      both cell types. Moreover, the oxidative parameters (oxygen species, 
      malonaldehyde) were significantly increased, while the non-enzymatic GSH/GSSG 
      ratios and the activities of enzymatic antioxidants (superoxide dismutase, 
      glutathione peroxidase and catalase) were decreased. Interestingly, DATS 
      treatment can restore the WBC number by 267.1% and 304.8% while RBC number by 
      108.6% and 117.7% in 30,45 mg/k DATS treated groups. In summary, we demonstrated 
      that benzene-induced cytopenia was related to the apoptosis of PBMCs and BMCs, 
      and DATS treatment could prevent benzene-induced cytopenia by suppressing 
      oxidative stress-mediated cell apoptosis via the PI3K/Akt pathway.
CI  - Copyright (c) 2017. Published by Elsevier Ltd.
FAU - Han, Wenting
AU  - Han W
AD  - Institute of Toxicology, School of Public Health, Shandong University, Jinan, 
      Shandong 250012, China.
FAU - Wang, Shuo
AU  - Wang S
AD  - Institute of Toxicology, School of Public Health, Shandong University, Jinan, 
      Shandong 250012, China.
FAU - Jiang, Lulu
AU  - Jiang L
AD  - Institute of Toxicology, School of Public Health, Shandong University, Jinan, 
      Shandong 250012, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Institute of Toxicology, School of Public Health, Shandong University, Jinan, 
      Shandong 250012, China.
FAU - Li, Ming
AU  - Li M
AD  - Institute of Toxicology, School of Public Health, Shandong University, Jinan, 
      Shandong 250012, China.
FAU - Wang, Xujing
AU  - Wang X
AD  - Institute of Toxicology, School of Public Health, Shandong University, Jinan, 
      Shandong 250012, China.
FAU - Xie, Keqin
AU  - Xie K
AD  - Institute of Toxicology, School of Public Health, Shandong University, Jinan, 
      Shandong 250012, China. Electronic address: keqinx@sdu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170812
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Allyl Compounds)
RN  - 0 (Antioxidants)
RN  - 0 (Protective Agents)
RN  - 0 (Sulfides)
RN  - 0ZO1U5A3XX (diallyl trisulfide)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - J64922108F (Benzene)
SB  - IM
MH  - Allyl Compounds/*pharmacology
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Apoptosis/drug effects
MH  - Benzene/*toxicity
MH  - Cell Line, Tumor
MH  - Leukocytes, Mononuclear
MH  - Male
MH  - Oxidative Stress/drug effects
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Protective Agents/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sulfides/*pharmacology
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - Benzene poisoning
OT  - Cytopenia
OT  - Diallyl trisulfide
OT  - Oxidative stress
EDAT- 2017/08/16 06:00
MHDA- 2018/02/17 06:00
CRDT- 2017/08/16 06:00
PHST- 2016/08/31 00:00 [received]
PHST- 2017/07/19 00:00 [revised]
PHST- 2017/07/19 00:00 [accepted]
PHST- 2017/08/16 06:00 [pubmed]
PHST- 2018/02/17 06:00 [medline]
PHST- 2017/08/16 06:00 [entrez]
AID - S0269-7491(16)31092-2 [pii]
AID - 10.1016/j.envpol.2017.07.069 [doi]
PST - ppublish
SO  - Environ Pollut. 2017 Dec;231(Pt 1):301-310. doi: 10.1016/j.envpol.2017.07.069. 
      Epub 2017 Aug 12.