PMID- 28810257
OWN - NLM
STAT- MEDLINE
DCOM- 20171020
LR  - 20171020
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 152
IP  - 3
DP  - 2017
TI  - Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra 
      Determined by Fluorescence in situ Hybridization.
PG  - 158-165
LID - 10.1159/000479179 [doi]
AB  - Whole-genome shotgun reads were analyzed to determine the repeat sequence 
      composition in the genome of black mustard, Brassica nigra (L.) Koch. The 
      analysis showed that satellite DNA sequences are very abundant in the black 
      mustard genome. The distribution pattern of 7 new tandem repeats (BnSAT13, 
      BnSAT28, BnSAT68, BnSAT76, BnSAT114, BnSAT180, and BnSAT200) on black mustard 
      chromosomes was visualized using fluorescence in situ hybridization (FISH). The 
      FISH signals of BnSAT13 and BnSAT76 provided useful cytogenetic markers; their 
      position and fluorescence intensity allowed for unambiguous identification of all 
      8 somatic metaphase chromosomes. A karyotype showing the location and 
      fluorescence intensity of these tandem repeat sequences together with the 
      position of rDNAs and centromeric retrotransposons of Brassica (CRB) was 
      constructed. The establishment of the FISH-based karyotype in B. nigra provides 
      valuable information that can be used in detailed analyses of B. nigra accessions 
      and derived allopolyploid Brassica species containing the B genome.
CI  - (c) 2017 S. Karger AG, Basel.
FAU - Wang, Gui-Xiang
AU  - Wang GX
AD  - Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry 
      Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural 
      Crops (North China), Ministry of Agriculture, Beijing Key Laboratory of Vegetable 
      Germplasm Improvement, Beijing, China.
FAU - He, Qun-Yan
AU  - He QY
FAU - Macas, Jiri
AU  - Macas J
FAU - Novak, Petr
AU  - Novak P
FAU - Neumann, Pavel
AU  - Neumann P
FAU - Meng, De-Xuan
AU  - Meng DX
FAU - Zhao, Hong
AU  - Zhao H
FAU - Guo, Ning
AU  - Guo N
FAU - Han, Shuo
AU  - Han S
FAU - Zong, Mei
AU  - Zong M
FAU - Jin, Wei-Wei
AU  - Jin WW
FAU - Liu, Fan
AU  - Liu F
LA  - eng
PT  - Journal Article
DEP - 20170816
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA, Satellite)
RN  - 0 (Genetic Markers)
RN  - 0 (Retroelements)
SB  - IM
MH  - Centromere
MH  - Chromosomes, Plant/genetics/ultrastructure
MH  - DNA, Plant/*genetics
MH  - DNA, Ribosomal/genetics
MH  - DNA, Satellite/genetics
MH  - Genetic Markers
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Karyotype
MH  - Metaphase
MH  - Microscopy, Fluorescence
MH  - Mustard Plant/*genetics
MH  - Retroelements
MH  - Tandem Repeat Sequences/*genetics
OTO - NOTNLM
OT  - (Peri)centromere
OT  - Brassica nigra
OT  - Fluorescence in situ hybridization
OT  - Karyotype
OT  - Repeat sequence
EDAT- 2017/08/16 06:00
MHDA- 2017/10/21 06:00
CRDT- 2017/08/16 06:00
PHST- 2017/05/15 00:00 [accepted]
PHST- 2017/08/16 06:00 [pubmed]
PHST- 2017/10/21 06:00 [medline]
PHST- 2017/08/16 06:00 [entrez]
AID - 000479179 [pii]
AID - 10.1159/000479179 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2017;152(3):158-165. doi: 10.1159/000479179. Epub 2017 Aug 
      16.