PMID- 28811017
OWN - NLM
STAT- MEDLINE
DCOM- 20180702
LR  - 20181202
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 983
DP  - 2017 Aug 29
TI  - Improved methodology for analysis of multiple phytohormones using sequential 
      magnetic solid-phase extraction coupled with liquid chromatography-tandem mass 
      spectrometry.
PG  - 112-120
LID - S0003-2670(17)30723-7 [pii]
LID - 10.1016/j.aca.2017.06.019 [doi]
AB  - Phytohormones are special small molecules that play important role in plant 
      growth and development at trace levels. Quantification of multiple phytohormones 
      will be great helpful for researches about cross-talks that plant hormones 
      regulate the responses of plants against both biotic and abiotic stresses by 
      means of synergistic or antagonistic interactions. In the current study, we 
      developed a method for profiling of phytohormones in one small sample, including 
      indole-3-acetic acid, abscisic acid, jasmonic acid, gibberellins, cytokinins and 
      brassinosteroids. These phytohormones mentioned above were firstly purified and 
      separated by sequential magnetic solid-phase extraction (MSPE) and then analyzed 
      by ultra-high performance liquid chromatography-electrospray tandem mass 
      spectrometry (UHPLC-MS/MS). Under the optimized extraction conditions, good 
      linearity was obtained with correlation coefficients (r) ranging from 0.9961 to 
      0.9998. The limits of detection (LODs, S/N = 3) were ranged from 0.45 to 
      126.1 pg mL(-1). The recoveries were between 85.0% and 116.2%. The relative 
      standard deviations (RSDs) were ranged from 2.7% to 16.1%. With the proposed 
      strategy, 23 phytohormones could be purified and analyzed from a single plant 
      extract. Finally, 16 phytohormones could be detected in 100 mg (fresh weight) 
      flower of Brassica napus L., including IAA, ABA, JA, 4 GAs, 3 BRs and 6 CKs with 
      the concentration ranged from 0.09 to 305.23 ng g(-1).
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Luo, Xiao-Tong
AU  - Luo XT
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Cai, Bao-Dong
AU  - Cai BD
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Chen, Xi
AU  - Chen X
AD  - Wuhan Institute of Biotechnology, Wuhan 430075, China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, China. 
      Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170619
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Plant Growth Regulators)
SB  - IM
MH  - *Chromatography, High Pressure Liquid
MH  - Plant Growth Regulators/*analysis
MH  - *Solid Phase Extraction
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Magnetic solid-phase extraction
OT  - Mass spectrometry
OT  - Phytohormones
EDAT- 2017/08/16 06:00
MHDA- 2018/07/03 06:00
CRDT- 2017/08/17 06:00
PHST- 2017/02/22 00:00 [received]
PHST- 2017/06/03 00:00 [revised]
PHST- 2017/06/11 00:00 [accepted]
PHST- 2017/08/17 06:00 [entrez]
PHST- 2017/08/16 06:00 [pubmed]
PHST- 2018/07/03 06:00 [medline]
AID - S0003-2670(17)30723-7 [pii]
AID - 10.1016/j.aca.2017.06.019 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2017 Aug 29;983:112-120. doi: 10.1016/j.aca.2017.06.019. Epub 
      2017 Jun 19.