PMID- 28812170
OWN - NLM
STAT- MEDLINE
DCOM- 20180618
LR  - 20220410
IS  - 1573-9686 (Electronic)
IS  - 0090-6964 (Linking)
VI  - 45
IP  - 11
DP  - 2017 Nov
TI  - A Novel Approach to Utilize Icariin as Icariin-Derived ECM on Small Intestinal 
      Submucosa Scaffold for Bone Repair.
PG  - 2673-2682
LID - 10.1007/s10439-017-1900-y [doi]
AB  - Icariin (Ic) has been demonstrated as a potent osteoinductive compound for bone 
      tissue engineering. However, toxic side effects of the drug and poor 
      biocompatibility of drug delivery systems (DDSs) still limit its application for 
      bone repair in the clinic. To overcome these disadvantages and utilize the 
      osteoinductivity of Ic, we developed a novel method to utilize Ic as an 
      Ic-derived osteoinductive extracellular matrix (ECM) on small intestinal 
      submucosa (SIS) (Ic-ECM-SIS). The generated Ic-ECM-SIS scaffolds, as a natural 
      construct, exhibited much better biocompatibility (including cell adhesion, cell 
      survival and cell proliferation) than Ic-SIS scaffolds generated by traditional 
      DDSs. Meanwhile, osteogenic differentiation was promoted by Ic-ECM-SIS with 
      higher expression of alkaline phosphatase, bone sialoprotein and osteocalcin than 
      ECM-SIS, which was same as Ic-SIS. BMP-4 expression was further increased in the 
      cells on Ic-ECM-SIS compared to that on Ic-SIS. A mouse calvarial defect model 
      was introduced to evaluate the function of Ic-ECM-SIS on bone regeneration in 
      vivo. The bone regeneration was enhanced in the defects implanted with 
      Ic-ECM-SIS, with a higher new bone formation ratio (BV/TV) than the defects 
      implanted with ECM-SIS or Ic-SIS. Angiogenesis was also promoted by Ic-ECM-SIS 
      implantation when compared with ECM-SIS or Ic-SIS. Thus, this work proposes a 
      novel method for applying a drug as a drug-derived ECM-modified scaffold for bone 
      tissue engineering.
FAU - Li, Mei
AU  - Li M
AD  - Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, 
      818 Fenghua Road, Ningbo, 315211, Zhejiang, People's Republic of China.
AD  - Ningbo Institute of Medical Sciences, Ningbo, 315020, Zhejiang, People's Republic 
      of China.
FAU - Zhang, Chi
AU  - Zhang C
AD  - Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, 
      818 Fenghua Road, Ningbo, 315211, Zhejiang, People's Republic of China.
FAU - Zhong, Yi
AU  - Zhong Y
AD  - Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, 
      818 Fenghua Road, Ningbo, 315211, Zhejiang, People's Republic of China.
FAU - Zhao, Jiyuan
AU  - Zhao J
AUID- ORCID: 0000-0002-1327-7119
AD  - Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, 
      818 Fenghua Road, Ningbo, 315211, Zhejiang, People's Republic of China. 
      zhaojiyuan@nbu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170815
PL  - United States
TA  - Ann Biomed Eng
JT  - Annals of biomedical engineering
JID - 0361512
RN  - 0 (Flavonoids)
RN  - VNM47R2QSQ (icariin)
SB  - IM
MH  - Animals
MH  - *Bone Regeneration
MH  - Cell Adhesion/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - *Extracellular Matrix
MH  - Flavonoids/*pharmacology
MH  - *Intestinal Mucosa
MH  - *Intestine, Small
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Osteoblasts/drug effects/physiology
MH  - Tissue Engineering
MH  - *Tissue Scaffolds
OTO - NOTNLM
OT  - Biocompatibility
OT  - Bone tissue engineering
OT  - Extracellular matrix (ECM)
OT  - Osteoblast
OT  - Osteoconductivity
OT  - Osteoinductivity
EDAT- 2017/08/16 06:00
MHDA- 2018/06/19 06:00
CRDT- 2017/08/17 06:00
PHST- 2017/05/26 00:00 [received]
PHST- 2017/08/09 00:00 [accepted]
PHST- 2017/08/16 06:00 [pubmed]
PHST- 2018/06/19 06:00 [medline]
PHST- 2017/08/17 06:00 [entrez]
AID - 10.1007/s10439-017-1900-y [pii]
AID - 10.1007/s10439-017-1900-y [doi]
PST - ppublish
SO  - Ann Biomed Eng. 2017 Nov;45(11):2673-2682. doi: 10.1007/s10439-017-1900-y. Epub 
      2017 Aug 15.