PMID- 28813499
OWN - NLM
STAT- MEDLINE
DCOM- 20171013
LR  - 20240326
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 8
DP  - 2017
TI  - Staphylococcus aureus biofilm elicits the expansion, activation and polarization 
      of myeloid-derived suppressor cells in vivo and in vitro.
PG  - e0183271
LID - 10.1371/journal.pone.0183271 [doi]
LID - e0183271
AB  - Staphylococcus aureus (S. aureus) is one of the most common causes of biofilm 
      infections in periprosthetic joint infections (PJIs). Accumulating evidence has 
      shown that the immunosuppressive environment established by S. aureus biofilm 
      infection in PJIs involves the presence of myeloid-derived suppressor cells 
      (MDSCs) and M2-macrophages. Due to the diversity of MDSCs, little is known about 
      whether S. aureus biofilm preferentially expands specific MDSC subsets or whether 
      MDSCs can further differentiate into M2-macrophages during S. aureus biofilm 
      infection. Here, we show that in agreement with the results from an established 
      rat PJI model, S. aureus biofilm cocultured with freshly isolated bone marrow 
      cells (BMCs) in vitro significantly increases the proportions of MDSCs, total 
      macrophages and M2-macrophages. Interestingly, we find that treatment of the BMCs 
      in vitro with S. aureus biofilm preferentially promotes the expansion of 
      monocytic MDSCs but not granulocytic MDSCs. Biofilm treatment also substantially 
      enhances the overall MDSC immunosuppressive activity in addition to the MDSC 
      expansion in vitro. Importantly, we provide evidence that S. aureus biofilm is 
      capable of further stimulating the conversion of monocytic MDSCs into 
      M2-macrophages in vitro and in vivo. Collectively, our studies reveal a direct 
      link between MDSCs and M2-macrophages occurring in S. aureus-associated PJIs.
FAU - Peng, Kuo-Ti
AU  - Peng KT
AD  - Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan.
FAU - Hsieh, Ching-Chuan
AU  - Hsieh CC
AD  - Department of Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan.
FAU - Huang, Tsung-Yu
AU  - Huang TY
AD  - Division of Infection Disease, Department of Internal Medicine, Chang Gung 
      Memorial Hospital, Chiayi, Taiwan.
AD  - Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung 
      University, Taoyuan, Taiwan.
FAU - Chen, Pei-Chun
AU  - Chen PC
AD  - Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Chiayi, Taiwan.
FAU - Shih, Hsin-Nung
AU  - Shih HN
AD  - Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
FAU - Lee, Mel S
AU  - Lee MS
AD  - Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Kaohsiung, 
      Taiwan.
FAU - Chang, Pey-Jium
AU  - Chang PJ
AUID- ORCID: 0000-0002-6492-0346
AD  - Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung 
      University, Taoyuan, Taiwan.
AD  - Department of Nephrology, Chang-Gung Memorial Hospital, Chiayi, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20170816
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Biofilms/*growth & development
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - Macrophages/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Scanning
MH  - Myeloid Cells/*cytology
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Staphylococcus aureus/*physiology
MH  - T-Lymphocytes/metabolism
MH  - T-Lymphocytes, Regulatory/metabolism
PMC - PMC5559065
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/08/17 06:00
MHDA- 2017/10/14 06:00
PMCR- 2017/08/16
CRDT- 2017/08/17 06:00
PHST- 2017/05/07 00:00 [received]
PHST- 2017/08/01 00:00 [accepted]
PHST- 2017/08/17 06:00 [entrez]
PHST- 2017/08/17 06:00 [pubmed]
PHST- 2017/10/14 06:00 [medline]
PHST- 2017/08/16 00:00 [pmc-release]
AID - PONE-D-17-17582 [pii]
AID - 10.1371/journal.pone.0183271 [doi]
PST - epublish
SO  - PLoS One. 2017 Aug 16;12(8):e0183271. doi: 10.1371/journal.pone.0183271. 
      eCollection 2017.