PMID- 28815023
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231112
IS  - 2047-2994 (Electronic)
IS  - 2047-2994 (Linking)
VI  - 6
DP  - 2017
TI  - Antibiotic resistance, ability to form biofilm and susceptibility to copper 
      alloys of selected staphylococcal strains isolated from touch surfaces in Polish 
      hospital wards.
PG  - 80
LID - 10.1186/s13756-017-0240-x [doi]
LID - 80
AB  - BACKGROUND: Despite the employment of sanitary regimes, contact transmission of 
      the aetiological agents of hospital infections is still exceedingly common. The 
      issue of microbe transmission becomes particularly important when facing 
      multidrug-resistant microorganisms such as methicillin-resistant staphylococci. 
      In the case of deficiencies in cleaning and disinfection procedures, hospital 
      equipment made of copper alloys can play an important role, complementing 
      traditional hospital hygiene procedures. The objective of this study was to 
      characterize staphylococcal strains isolated from touch surfaces in Polish 
      hospital wards in terms of their drug resistance, ability to form biofilm and 
      susceptibility to antimicrobial activity of copper alloys. METHODS: The materials 
      for the study were 95 staphylococcal strains isolated from touch surfaces in 13 
      different hospital wards from Malopolska province (the south of Poland). 
      Phenotypic and genotypic antibiotic resistance were checked for erythromycin, 
      clindamycin, gentamycin, ciprofloxacin, trimethoprim/sulfamethoxazole and 
      mupirocin. Biofilm formation ability for the tested strains was checked with the 
      use of culture on Congo red agar. Susceptibility to copper, tin bronze, brass and 
      new silver was tested using a modification of the Japanese standard. RESULTS: 
      Over 67% of the analysed staphylococcal strains were methicillin-resistant (MR). 
      Four strains were resistant to all of the tested antibiotics, and 14 were 
      resistant to all except mupirocin. Strains classified as MR had significantly 
      increased resistance to the remaining antibiotic groups. About one-third of the 
      analysed strains revealed biofilm-forming ability. Among the majority of species, 
      biofilm-forming and non-biofilm-forming strains were distributed evenly; in the 
      case of S. haemolyticus only, negative strains accounted for 92.8%. 
      Susceptibility to copper alloys was different between strains and rather lower 
      than in the case of the SA strain selected for comparison. CONCLUSIONS: 
      Coagulase-negative staphylococci, the most commonly isolated in Polish hospital 
      wards, should not be neglected as an infection risk factor due their high 
      antibiotic resistance. Our experiments confirmed that touch surfaces made of 
      copper alloys may play an important role in eliminating bacteria from the 
      hospital environment.
FAU - Rozanska, Anna
AU  - Rozanska A
AD  - Jagiellonian University Medical College, Faculty of Medicine, Chair of 
      Microbiology, Czysta str. 18, 31-121 Krakow, Poland. ISNI: 0000 0001 2162 9631. 
      GRID: grid.5522.0
FAU - Chmielarczyk, Agnieszka
AU  - Chmielarczyk A
AD  - Jagiellonian University Medical College, Faculty of Medicine, Chair of 
      Microbiology, Czysta str. 18, 31-121 Krakow, Poland. ISNI: 0000 0001 2162 9631. 
      GRID: grid.5522.0
FAU - Romaniszyn, Dorota
AU  - Romaniszyn D
AD  - Jagiellonian University Medical College, Faculty of Medicine, Chair of 
      Microbiology, Czysta str. 18, 31-121 Krakow, Poland. ISNI: 0000 0001 2162 9631. 
      GRID: grid.5522.0
FAU - Bulanda, Malgorzata
AU  - Bulanda M
AD  - Jagiellonian University Medical College, Faculty of Medicine, Chair of 
      Microbiology, Czysta str. 18, 31-121 Krakow, Poland. ISNI: 0000 0001 2162 9631. 
      GRID: grid.5522.0
FAU - Walkowicz, Monika
AU  - Walkowicz M
AD  - Faculty of Non-Ferrous Metals, Department of Metal Working and Physical 
      Metallurgy of Non-Ferrous Metals, AGH University of Science and Technology, al. 
      Mickiewicza 30, 30-059 Krakow, Poland. ISNI: 0000 0000 9174 1488. GRID: 
      grid.9922.0
FAU - Osuch, Piotr
AU  - Osuch P
AD  - Faculty of Non-Ferrous Metals, Department of Metal Working and Physical 
      Metallurgy of Non-Ferrous Metals, AGH University of Science and Technology, al. 
      Mickiewicza 30, 30-059 Krakow, Poland. ISNI: 0000 0000 9174 1488. GRID: 
      grid.9922.0
FAU - Knych, Tadeusz
AU  - Knych T
AD  - Faculty of Non-Ferrous Metals, Department of Metal Working and Physical 
      Metallurgy of Non-Ferrous Metals, AGH University of Science and Technology, al. 
      Mickiewicza 30, 30-059 Krakow, Poland. ISNI: 0000 0000 9174 1488. GRID: 
      grid.9922.0
LA  - eng
PT  - Journal Article
DEP - 20170814
PL  - England
TA  - Antimicrob Resist Infect Control
JT  - Antimicrobial resistance and infection control
JID - 101585411
PMC - PMC5556671
OTO - NOTNLM
OT  - Antibiotic resistance
OT  - Antimicrobial copper
OT  - Biofilm-forming staphylococci
OT  - Hospital environment contamination
OT  - Staphylococci
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable CONSENT FOR 
      PUBLICATION: Not applicable COMPETING INTERESTS: The authors declare that they 
      have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral 
      with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2017/08/18 06:00
MHDA- 2017/08/18 06:01
PMCR- 2017/08/14
CRDT- 2017/08/18 06:00
PHST- 2017/05/26 00:00 [received]
PHST- 2017/08/04 00:00 [accepted]
PHST- 2017/08/18 06:00 [entrez]
PHST- 2017/08/18 06:00 [pubmed]
PHST- 2017/08/18 06:01 [medline]
PHST- 2017/08/14 00:00 [pmc-release]
AID - 240 [pii]
AID - 10.1186/s13756-017-0240-x [doi]
PST - epublish
SO  - Antimicrob Resist Infect Control. 2017 Aug 14;6:80. doi: 
      10.1186/s13756-017-0240-x. eCollection 2017.