PMID- 28815295
OWN - NLM
STAT- MEDLINE
DCOM- 20190222
LR  - 20190222
IS  - 1863-2661 (Electronic)
IS  - 1863-2653 (Linking)
VI  - 223
IP  - 1
DP  - 2018 Jan
TI  - Loss of Kirrel family members alters glomerular structure and synapse numbers in 
      the accessory olfactory bulb.
PG  - 307-319
LID - 10.1007/s00429-017-1485-0 [doi]
AB  - The accessory olfactory system controls social and sexual behaviours in mice, 
      both of which are critical for their survival. Vomeronasal sensory neuron (VSN) 
      axons form synapses with mitral cell dendrites in glomeruli of the accessory 
      olfactory bulb (AOB). Axons of VSNs expressing the same vomeronasal receptor (VR) 
      converge into multiple glomeruli within spatially conserved regions of the AOB. 
      Here, we have examined the role of the cell adhesion molecule Kirrel2 in the 
      formation of glomeruli within the AOB. We find that Kirrel2 expression is 
      dispensable for early axonal guidance events, such as fasciculation of the 
      vomeronasal tract and segregation of apical and basal VSN axons into the anterior 
      and posterior regions of the AOB, but is necessary for glomeruli formation. 
      Specific ablation of Kirrel2 expression in VSN axons results in the 
      disorganization of the glomerular layer of the posterior AOB and in the formation 
      of fewer and larger glomeruli. Furthermore, simultaneous ablation of Kirrel2 and 
      Kirrel3 expression leads to a loss of morphologically identifiable glomeruli in 
      the AOB, reduced excitatory synapse numbers, and larger presynaptic terminals. 
      Taken together, our results demonstrate that Kirrel2 and Kirrel3 are essential 
      for the formation of glomeruli and suggest they contribute to synaptogenesis in 
      the AOB.
FAU - Brignall, Alexandra C
AU  - Brignall AC
AD  - Montreal Neurological Institute, 3801 University, Montreal, QC, H3A 2B4, Canada.
AD  - Department of Anatomy and Cell Biology, McGill University, Montreal, Canada.
FAU - Raja, Reesha
AU  - Raja R
AD  - Montreal Neurological Institute, 3801 University, Montreal, QC, H3A 2B4, Canada.
AD  - Department of Neurology and Neurosurgery, McGill University, Montreal, Canada.
FAU - Phen, Alina
AU  - Phen A
AD  - Department of Anatomy and Cell Biology, McGill University, Montreal, Canada.
FAU - Prince, Janet E A
AU  - Prince JEA
AD  - Montreal Neurological Institute, 3801 University, Montreal, QC, H3A 2B4, Canada.
AD  - Department of Neurology and Neurosurgery, McGill University, Montreal, Canada.
FAU - Dumontier, Emilie
AU  - Dumontier E
AD  - Montreal Neurological Institute, 3801 University, Montreal, QC, H3A 2B4, Canada.
FAU - Cloutier, Jean-Francois
AU  - Cloutier JF
AUID- ORCID: 0000-0003-0840-2795
AD  - Montreal Neurological Institute, 3801 University, Montreal, QC, H3A 2B4, Canada. 
      jf.cloutier@mcgill.ca.
AD  - Department of Neurology and Neurosurgery, McGill University, Montreal, Canada. 
      jf.cloutier@mcgill.ca.
AD  - Department of Anatomy and Cell Biology, McGill University, Montreal, Canada. 
      jf.cloutier@mcgill.ca.
LA  - eng
PT  - Journal Article
DEP - 20170816
PL  - Germany
TA  - Brain Struct Funct
JT  - Brain structure & function
JID - 101282001
RN  - 0 (Kirrel3 protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Olfactory Marker Protein)
RN  - 0 (Omp protein, mouse)
RN  - 0 (Slc17a6 protein, mouse)
RN  - 0 (Vesicular Glutamate Transport Protein 2)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - GTP-Binding Proteins/metabolism
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Membrane Proteins/*deficiency/genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Microscopy, Electron
MH  - Olfactory Bulb/*cytology/ultrastructure
MH  - Olfactory Marker Protein/genetics/metabolism
MH  - Olfactory Pathways/metabolism
MH  - Presynaptic Terminals/metabolism/ultrastructure
MH  - Sensory Receptor Cells/*cytology/metabolism
MH  - Statistics, Nonparametric
MH  - Synapses/*metabolism/ultrastructure
MH  - Vesicular Glutamate Transport Protein 2
OTO - NOTNLM
OT  - Accessory olfactory bulb
OT  - Axonal coalescence
OT  - Axonal guidance
OT  - Kirrel
OT  - Neph
OT  - Sensory neurons
OT  - Synapse
OT  - Vomeronasal
EDAT- 2017/08/18 06:00
MHDA- 2019/02/23 06:00
CRDT- 2017/08/18 06:00
PHST- 2016/11/01 00:00 [received]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/08/18 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
PHST- 2017/08/18 06:00 [entrez]
AID - 10.1007/s00429-017-1485-0 [pii]
AID - 10.1007/s00429-017-1485-0 [doi]
PST - ppublish
SO  - Brain Struct Funct. 2018 Jan;223(1):307-319. doi: 10.1007/s00429-017-1485-0. Epub 
      2017 Aug 16.