PMID- 28815364
OWN - NLM
STAT- MEDLINE
DCOM- 20180724
LR  - 20181202
IS  - 1573-6814 (Electronic)
IS  - 1389-9333 (Linking)
VI  - 18
IP  - 4
DP  - 2017 Dec
TI  - Long bone mesenchymal stem cells (Lb-MSCs): clinically reliable cells for 
      osteo-diseases.
PG  - 489-500
LID - 10.1007/s10561-017-9652-3 [doi]
AB  - Mesenchymal stem cells (MSCs) have been designated as the most reliable cells in 
      clinics to treat osteo-diseases because of their versatile nature. MSCs, isolated 
      from long bone (Lb-MSCs) are rarely reported and named as RIA-MSCs because of the 
      reamer-irrigator-aspirator (RIA) device. The potential of these cells in the 
      treatment of non-union bone fractures made them the ideal candidates to be 
      studied for clinical practices. In this work, effect of cryopreservation on the 
      proliferation and differentiation capabilities of long bone MSCs (Lb-MSCs) has 
      been studied. For this purpose, Lb-MSCs were isolated via RIA device and 
      characterized using flow cytometry and differentiation assays. Cells were 
      cryopreserved for 3, 6 and 12 months and thereafter were characterized using 
      differentiation assays and genetic markers specific for osteogenic, chondrogenic, 
      and adipogenic potential quantitatively by qRT-PCR. Lb-MSCs were found expressing 
      MSC characteristic markers defining their identity. The population doubling time 
      (PDT) was about 2.5 +/- 0.5 days and colonies appeared after 7-10 days. 
      Differentiation potential and gene expression of 3, 6 and 12 months cryopreserved 
      Lb-MSCs were unaltered. The results show that cryopreservation did not have an 
      effect on the differentiation potential of human Lb-MSCs. Therefore, our work 
      offers Lb-MSCs as clinically cells for treating osteo-diseases.
FAU - Toosi, Shirin
AU  - Toosi S
AD  - Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, 
      Iran.
FAU - Naderi-Meshkin, Hojjat
AU  - Naderi-Meshkin H
AD  - Stem Cell and Regenerative Medicine Research Group, Academic Center for 
      Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.
FAU - Kalalinia, Fatemeh
AU  - Kalalinia F
AD  - Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, 
      Iran.
AD  - Medical Genetic Research Center, Medical School, Mashhad University of Medical 
      Sciences, Mashhad, Iran.
FAU - Pievandi, Mohammad Taghi
AU  - Pievandi MT
AD  - Department of Orthopedic Surgery, Orthopedic and Trauma Research Center, Mashhad 
      University of Medical Sciences, Mashhad, Iran.
FAU - Hosseinkhani, Hossein
AU  - Hosseinkhani H
AD  - School of Biomedical Engineering, National Yang Ming University, Taipei, Taiwan.
FAU - Bahrami, Ahmad Reza
AU  - Bahrami AR
AD  - Stem Cell and Regenerative Medicine Research Group, Academic Center for 
      Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.
FAU - Heirani-Tabasi, Asieh
AU  - Heirani-Tabasi A
AD  - Stem Cell and Regenerative Medicine Research Group, Academic Center for 
      Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.
FAU - Mirahmadi, Mahdi
AU  - Mirahmadi M
AD  - Stem Cell and Regenerative Medicine Research Group, Academic Center for 
      Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.
FAU - Behravan, Javad
AU  - Behravan J
AD  - Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, 
      Iran. Behravanj@mums.ac.ir.
AD  - Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad, Iran. 
      Behravanj@mums.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20170816
PL  - Netherlands
TA  - Cell Tissue Bank
JT  - Cell and tissue banking
JID - 100965121
SB  - IM
MH  - Bone and Bones/cytology
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/*physiology
MH  - Cell Proliferation/*physiology
MH  - Cells, Cultured
MH  - Chondrogenesis/physiology
MH  - Cryopreservation/methods
MH  - Diastasis, Bone/physiopathology
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Osteogenesis/*physiology
OTO - NOTNLM
OT  - Cell banking
OT  - Cryopreservation
OT  - Differentiation
OT  - Long bone
OT  - Mesenchymal stem cells
OT  - Stem cell therapy
EDAT- 2017/08/18 06:00
MHDA- 2018/07/25 06:00
CRDT- 2017/08/18 06:00
PHST- 2016/12/25 00:00 [received]
PHST- 2017/08/08 00:00 [accepted]
PHST- 2017/08/18 06:00 [pubmed]
PHST- 2018/07/25 06:00 [medline]
PHST- 2017/08/18 06:00 [entrez]
AID - 10.1007/s10561-017-9652-3 [pii]
AID - 10.1007/s10561-017-9652-3 [doi]
PST - ppublish
SO  - Cell Tissue Bank. 2017 Dec;18(4):489-500. doi: 10.1007/s10561-017-9652-3. Epub 
      2017 Aug 16.