PMID- 28815806
OWN - NLM
STAT- MEDLINE
DCOM- 20180126
LR  - 20180126
IS  - 1099-0461 (Electronic)
IS  - 1095-6670 (Linking)
VI  - 31
IP  - 11
DP  - 2017 Nov
TI  - Evaluation in zebrafish model of the toxicity of rhodamine B-conjugated 
      crotamine, a peptide potentially useful for diagnostics and therapeutics.
LID - 10.1002/jbt.21964 [doi]
AB  - Crotamine is defensin-like cationic peptide from rattlesnake venom that possesses 
      anticancer, antimicrobial, and antifungal properties. Despite these promising 
      biological activities, toxicity is a major concern associated with the 
      development of venom-derived peptides as therapeutic agents. In the present 
      study, we used zebrafish as a system model to evaluate the toxicity of rhodamine 
      B-conjugated (RhoB) crotamine derivative. The lethal toxic concentration of 
      RhoB-crotamine was as low as 4 muM, which effectively kill zebrafish larvae in 
      less than 10 min. With non-lethal concentrations (<1 muM), crotamine caused 
      malformation in zebrafish embryos, delayed or completely halted hatching, 
      adversely affected embryonic developmental programming, decreased the cardiac 
      functions, and attenuated the swimming distance of zebrafish. The RhoB-crotamine 
      translocated across vitelline membrane and accumulated in zebrafish yolk sac. 
      These results demonstrate the sensitive responsivity of zebrafish to trial 
      crotamine analogues for the development of novel therapeutic peptides with 
      improved safety, bioavailability, and efficacy profiles.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Chan, Judy Yuet-Wa
AU  - Chan JY
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Zhou, Hefeng
AU  - Zhou H
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Kwan, Yiu Wa
AU  - Kwan YW
AD  - School of Biomedical Sciences, Faculty of Medicine, The Chinese University of 
      Hong Kong, Shatin, Hong Kong, China.
FAU - Chan, Shun Wan
AU  - Chan SW
AD  - State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department 
      of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, 
      Kowloon, Hong Kong, China.
FAU - Radis-Baptista, Gandhi
AU  - Radis-Baptista G
AUID- ORCID: 0000-0001-9210-092X
AD  - Laboratory of Biochemistry and Biotechnology, Institute for Marine Sciences, 
      Federal University of Ceara, Fortaleza, Brazil.
FAU - Lee, Simon Ming-Yuen
AU  - Lee SM
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
LA  - eng
PT  - Journal Article
DEP - 20170816
PL  - United States
TA  - J Biochem Mol Toxicol
JT  - Journal of biochemical and molecular toxicology
JID - 9717231
RN  - 0 (Crotalid Venoms)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Rhodamines)
RN  - E58TBP78IH (crotamine)
RN  - K7G5SCF8IL (rhodamine B)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Crotalid Venoms/chemistry/pharmacokinetics/*toxicity
MH  - Embryo, Nonmammalian/drug effects
MH  - Fluorescent Dyes/chemistry/pharmacokinetics
MH  - Heart/drug effects/embryology
MH  - Larva/drug effects
MH  - Locomotion/drug effects
MH  - Rhodamines/*chemistry/pharmacokinetics
MH  - Tissue Distribution
MH  - Toxicity Tests/*methods
MH  - Zebrafish/*embryology
OTO - NOTNLM
OT  - acute toxicity
OT  - rhodamine B-crotamine
OT  - screening of pharmaceuticals
OT  - venom-derived peptides
OT  - zebrafish model
EDAT- 2017/08/18 06:00
MHDA- 2018/01/27 06:00
CRDT- 2017/08/18 06:00
PHST- 2017/05/10 00:00 [received]
PHST- 2017/07/06 00:00 [revised]
PHST- 2017/07/11 00:00 [accepted]
PHST- 2017/08/18 06:00 [pubmed]
PHST- 2018/01/27 06:00 [medline]
PHST- 2017/08/18 06:00 [entrez]
AID - 10.1002/jbt.21964 [doi]
PST - ppublish
SO  - J Biochem Mol Toxicol. 2017 Nov;31(11). doi: 10.1002/jbt.21964. Epub 2017 Aug 16.