PMID- 28816270
OWN - NLM
STAT- MEDLINE
DCOM- 20190220
LR  - 20190320
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 49
IP  - 4
DP  - 2017 Aug 18
TI  - [Clinical value of fluorescence in situ hybridization positive of exfoliated 
      urothelial cells in urothelial carcinoma].
PG  - 585-589
AB  - OBJECTIVE: To analyze the clinical pathologic characteristics of cases with 
      fluorescence in situ hybridization (FISH) positive of exfoliated urothelial 
      cells, so as to evaluate the clinical utility of FISH in the diagnosis of 
      urothelial carcinoma (UC). METHODS: A total of 271 cases of FISH positive in 
      Department of Urology of Peking University First Hospital from Apr. 2012 to Sep. 
      2015 were recruited in this study. Retrospective analysis was made on their 
      clinical data. For FISH analysis, labeled probes specific for chromosomes 3, 7, 
      17, and the p16 (9p21) gene were used to assess chromosomal abnormalities 
      indicative of malignancy. The positive predict values (PPV) of all the techniques 
      were analyzed. RESULTS: Of the 271 patients, 207 cases were UC, 7 cases were 
      non-UC, and 57 cases were benign diseases. The PPV of FISH in detecting UC was 
      76.4%, while the 95% confidence interval (CI) 71.3% to 81.5%. In the cohort of 
      FISH positive, this value was similar to that of urinary cytology (PPV 86.8%, 95% 
      CI: 78.5%-95.0%). The PPV of FISH was lower than that of cystoscopy and 
      ureteroscopy (PPV 96.1%, 95% CI: 91.7%-100.0%). There were significant 
      differences between this study and the PPV of FISH reported abroad (PPV 53.9%, 
      chi(2)=33.048, P<0.001). Of all the UC with FISH positive, bladder cancer showed an 
      earlier pathological stage versus renal pelvic carcinoma and ureteral carcinoma, 
      with significance (chi(2)=5.894, P=0.015, and chi(2)=13.601, P<0.001, respectively). 
      However, no difference was found in the size, pathological stage and pathological 
      grade of tumors between the urinary cytology positive group and the urinary 
      cytology negative group. The rate of high-grade UC in ureteral carcinoma of FISH 
      positive was 92.3%, much higher than that of ureteral carcinoma reported 
      domestically. CONCLUSION: The PPV of FISH in detecting UC is higher relatively, 
      with a better clinic value for Chinese patients. The ureteral carcinoma with FISH 
      positive obtains a higher pathological grade, which is of great guiding 
      significance for UC.
FAU - Liu, J Y
AU  - Liu JY
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
FAU - Peng, X
AU  - Peng X
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
FAU - Ning, X H
AU  - Ning XH
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
FAU - Li, T
AU  - Li T
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
FAU - Peng, S H
AU  - Peng SH
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
FAU - Wang, J Y
AU  - Wang JY
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
FAU - Liu, S J
AU  - Liu SJ
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
FAU - Ding, Y
AU  - Ding Y
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
FAU - Cai, L
AU  - Cai L
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
FAU - Gong, K
AU  - Gong K
AD  - Department of Urology, Peking University First Hospital; Institute of Urology, 
      Peking University; National Urological Cancer Center, Beijing 100034, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
SB  - IM
MH  - *Carcinoma, Transitional Cell/diagnosis/pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - *Urinary Bladder Neoplasms/diagnosis/pathology
MH  - Urologic Neoplasms
MH  - Urothelium
EDAT- 2017/08/18 06:00
MHDA- 2019/03/21 06:00
CRDT- 2017/08/18 06:00
PHST- 2017/08/18 06:00 [entrez]
PHST- 2017/08/18 06:00 [pubmed]
PHST- 2019/03/21 06:00 [medline]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2017 Aug 18;49(4):585-589.