PMID- 28816586
OWN - NLM
STAT- MEDLINE
DCOM- 20180906
LR  - 20180906
IS  - 1931-843X (Electronic)
IS  - 1540-9996 (Linking)
VI  - 27
IP  - 1
DP  - 2018 Jan
TI  - Oxidative Stress in the Visceral Fat Is Elevated in Postmenopausal Women with 
      Gynecologic Cancer.
PG  - 99-106
LID - 10.1089/jwh.2016.6301 [doi]
AB  - OBJECTIVES: This study aimed to evaluate differences in oxidative stress of 
      visceral fat between premenopausal and postmenopausal women and clarify the 
      antioxidant effect of estrogen on adipocytes. MATERIALS AND METHODS: Abdominal 
      subcutaneous and omental visceral adipose tissues were obtained from 38 patients 
      who underwent gynecological surgery. We measured the sizes of the adipocytes and 
      evaluated the lipid peroxidation levels in the adipose tissues. We investigated 
      whether estrogen inhibited the intracellular reactive oxygen species (ROS) 
      production that was induced by hydrogen peroxide (H(2)O(2)) in 3T3-L1 adipocytes. 
      RESULTS: The visceral adipocytes were 1162.4 mum(2) and 1881.9 mum(2) in 
      premenopausal and postmenopausal women, respectively; hence they were 
      significantly larger in the latter (p < 0.05). The lipid peroxidation levels were 
      46.7 nmoL/mg protein in premenopausal women and 99.6 nmoL/mg protein in 
      postmenopausal women; hence the lipid peroxidation levels were significantly 
      higher in the latter (p < 0.05). Estradiol (E2) significantly reduced the 
      intracellular ROS levels that were induced by H2O2 in 3T3-L1 adipocytes 
      (p < 0.01). We determined that E2 significantly increased the expression of 
      nuclear factor erythroid 2-related factor 2 (NRF2)-dependent antioxidant genes, 
      heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and the 
      glutamate-cysteine ligase (GCL) modifier subunit genes, in 3T3-L1 adipocytes 
      (p < 0.01). CONCLUSION: Oxidative stress in the visceral fat is higher in 
      postmenopausal women. The expression of the antioxidant genes HO-1, NQO1, and GCL 
      was upregulated by estrogen in 3T3-L1 adipocytes. Hence, estrogen may act as an 
      antioxidant in the adipose tissues of premenopausal women.
FAU - Narumi, Megumi
AU  - Narumi M
AD  - 1 Department of Obstetrics and Gynecology, Yamagata University Faculty of 
      Medicine , Yamagata, Japan .
FAU - Takahashi, Kazuhiro
AU  - Takahashi K
AD  - 1 Department of Obstetrics and Gynecology, Yamagata University Faculty of 
      Medicine , Yamagata, Japan .
FAU - Yamatani, Hizuru
AU  - Yamatani H
AD  - 1 Department of Obstetrics and Gynecology, Yamagata University Faculty of 
      Medicine , Yamagata, Japan .
FAU - Seino, Manabu
AU  - Seino M
AD  - 1 Department of Obstetrics and Gynecology, Yamagata University Faculty of 
      Medicine , Yamagata, Japan .
FAU - Yamanouchi, Keiko
AU  - Yamanouchi K
AD  - 1 Department of Obstetrics and Gynecology, Yamagata University Faculty of 
      Medicine , Yamagata, Japan .
FAU - Ohta, Tsuyoshi
AU  - Ohta T
AD  - 1 Department of Obstetrics and Gynecology, Yamagata University Faculty of 
      Medicine , Yamagata, Japan .
FAU - Takahashi, Toshifumi
AU  - Takahashi T
AD  - 1 Department of Obstetrics and Gynecology, Yamagata University Faculty of 
      Medicine , Yamagata, Japan .
FAU - Kurachi, Hirohisa
AU  - Kurachi H
AD  - 2 Osaka Medical Center and Research Institute for Maternal and Child Health , 
      Izumi, Osaka, Japan .
FAU - Nagase, Satoru
AU  - Nagase S
AD  - 1 Department of Obstetrics and Gynecology, Yamagata University Faculty of 
      Medicine , Yamagata, Japan .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170817
PL  - United States
TA  - J Womens Health (Larchmt)
JT  - Journal of women's health (2002)
JID - 101159262
RN  - 0 (Estrogens)
RN  - 0 (NF-E2-Related Factor 2)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (NQO1 protein, human)
RN  - EC 6.3.2.2 (Glutamate-Cysteine Ligase)
SB  - IM
MH  - 3T3-L1 Cells/*drug effects
MH  - Animals
MH  - Estrogens/*pharmacology
MH  - Female
MH  - Genital Neoplasms, Female/*complications
MH  - Glutamate-Cysteine Ligase
MH  - Heme Oxygenase-1/*drug effects
MH  - Humans
MH  - Hydrogen Peroxide
MH  - Intra-Abdominal Fat/*metabolism
MH  - Lipid Peroxidation
MH  - Mice
MH  - NAD(P)H Dehydrogenase (Quinone)
MH  - NF-E2-Related Factor 2/*drug effects
MH  - *Oxidative Stress
MH  - *Postmenopause
MH  - *Premenopause
OTO - NOTNLM
OT  - adipocyte
OT  - estrogen
OT  - menopause
OT  - oxidative stress
EDAT- 2017/08/18 06:00
MHDA- 2018/09/07 06:00
CRDT- 2017/08/18 06:00
PHST- 2017/08/18 06:00 [pubmed]
PHST- 2018/09/07 06:00 [medline]
PHST- 2017/08/18 06:00 [entrez]
AID - 10.1089/jwh.2016.6301 [doi]
PST - ppublish
SO  - J Womens Health (Larchmt). 2018 Jan;27(1):99-106. doi: 10.1089/jwh.2016.6301. 
      Epub 2017 Aug 17.