PMID- 28819363
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2017
DP  - 2017
TI  - Combined Analysis of Endothelial, Hematopoietic, and Mesenchymal Stem Cell 
      Compartments Shows Simultaneous but Independent Effects of Age and Heart Disease.
PG  - 5237634
LID - 10.1155/2017/5237634 [doi]
LID - 5237634
AB  - Clinical trials using stem cell therapy for heart diseases have not reproduced 
      the initial positive results obtained with animal models. This might be explained 
      by a decreased regenerative capacity of stem cells collected from the patients. 
      This work aimed at the simultaneous investigation of endothelial stem/progenitor 
      cells (EPCs), mesenchymal stem/progenitor cells (MSCs), and hematopoietic 
      stem/progenitor cells (HSCs) in sternal bone marrow samples of patients with 
      ischemic or valvular heart disease, using flow cytometry and colony assays. The 
      study included 36 patients referred for coronary artery bypass grafting or valve 
      replacement surgery. A decreased frequency of stem cells was observed in both 
      groups of patients. Left ventricular dysfunction, diabetes, and intermediate risk 
      in EuroSCORE and SYNTAX score were associated with lower EPCs frequency, and the 
      use of aspirin and beta-blockers correlated with a higher frequency of HSCs and 
      EPCs, respectively. Most importantly, the distribution of frequencies in the 
      three stem cell compartments showed independent patterns. The combined 
      investigation of the three stem cell compartments in patients with cardiovascular 
      diseases showed that they are independently affected by the disease, suggesting 
      the investigation of prognostic factors that may be used to determine when 
      autologous stem cells may be used in cell therapy.
FAU - Ghem, Carine
AU  - Ghem C
AUID- ORCID: 0000-0002-8550-5889
AD  - Institute of Cardiology of Rio Grande do Sul, Fundacao Universitaria de 
      Cardiologia, Av. Princesa Isabel 395, 90040-371 Porto Alegre, RS, Brazil.
FAU - Dias, Lucinara Dadda
AU  - Dias LD
AUID- ORCID: 0000-0002-2490-0412
AD  - Institute of Cardiology of Rio Grande do Sul, Fundacao Universitaria de 
      Cardiologia, Av. Princesa Isabel 395, 90040-371 Porto Alegre, RS, Brazil.
FAU - Sant'Anna, Roberto Tofani
AU  - Sant'Anna RT
AD  - Institute of Cardiology of Rio Grande do Sul, Fundacao Universitaria de 
      Cardiologia, Av. Princesa Isabel 395, 90040-371 Porto Alegre, RS, Brazil.
FAU - Kalil, Renato A K
AU  - Kalil RAK
AUID- ORCID: 0000-0001-9084-129X
AD  - Institute of Cardiology of Rio Grande do Sul, Fundacao Universitaria de 
      Cardiologia, Av. Princesa Isabel 395, 90040-371 Porto Alegre, RS, Brazil.
AD  - Universidade Federal de Ciencias da Saude de Porto Alegre, Rua Sarmento Leite 
      245, 90050-170 Porto Alegre, RS, Brazil.
FAU - Markoski, Melissa
AU  - Markoski M
AUID- ORCID: 0000-0003-2982-5850
AD  - Institute of Cardiology of Rio Grande do Sul, Fundacao Universitaria de 
      Cardiologia, Av. Princesa Isabel 395, 90040-371 Porto Alegre, RS, Brazil.
FAU - Nardi, Nance Beyer
AU  - Nardi NB
AUID- ORCID: 0000-0001-7739-1124
AD  - Institute of Cardiology of Rio Grande do Sul, Fundacao Universitaria de 
      Cardiologia, Av. Princesa Isabel 395, 90040-371 Porto Alegre, RS, Brazil.
AD  - Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, 
      Av. Farroupilha 8001, 92425-900 Canoas, RS, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170727
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC5551513
EDAT- 2017/08/19 06:00
MHDA- 2017/08/19 06:01
PMCR- 2017/07/27
CRDT- 2017/08/19 06:00
PHST- 2017/03/05 00:00 [received]
PHST- 2017/05/24 00:00 [revised]
PHST- 2017/06/06 00:00 [accepted]
PHST- 2017/08/19 06:00 [entrez]
PHST- 2017/08/19 06:00 [pubmed]
PHST- 2017/08/19 06:01 [medline]
PHST- 2017/07/27 00:00 [pmc-release]
AID - 10.1155/2017/5237634 [doi]
PST - ppublish
SO  - Stem Cells Int. 2017;2017:5237634. doi: 10.1155/2017/5237634. Epub 2017 Jul 27.