PMID- 28819444 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 1837-9664 (Print) IS - 1837-9664 (Electronic) IS - 1837-9664 (Linking) VI - 8 IP - 12 DP - 2017 TI - Association of HLA-DRB1, HLA-DQB1 Polymorphisms with HPV 16 E6 Variants among Young Cervical Cancer Patients in China. PG - 2401-2409 LID - 10.7150/jca.19809 [doi] AB - Potential correlation of human papillomavirus (HPV) 16 E6 variants and human leukocyte antigen (HLA) class II polymorphisms has been suggested in patients with cervical cancer, so far little information is available about the possible interaction between E6 variants and HLA class II variability during the obviously accelerated progression to cervical cancer in young women. In this study, we aimed to explore the association between the HPV16 E6 variants and HLA-DRB1, DQB1 alleles in a Chinese young cervical cancer population. The HLA-DRB1, HLA-DQB1 polymophisms were genotyped by low-resolution polymerase chain reaction (PCR) with sequence-specific primer. HPV16 E6 DNA was tested by Sanger fluorescent dye dideoxy-termination technique. The difference of DRB1, DQB1 polymorphisms between young cervical cancer patients (35ys, n=85) and the association with E6 variants were analyzed. Results showed that the distribution pattern of HLA-DRB1, DQB1 alleles was different between young cervical cancer patients and older ones. The allele frequency of DQB1*0501 in young patients was significantly lower than older ones (6.6% vs. 23.5%, p<0.05). The HPV16 E6 A4 lineage was the exclusive type observed in young patients, and its prevalence was significantly higher than that of older cases (82.86% vs.41.94%, p<0.05). DRB1*03 was not found in young patients positive for the HPV16 E6 A4 lineage, whereas it was observed in 19.2 % older patients with A4 positive(Pc<0.05). In conclusion, specific association between certain HPV16 E6 variant and genetic polymorphisms of HLA may play a role during the progression of early onset cervical cancer in young patients. Certain HLA-DRB1 and HLA-DQB1 alleles may be related to the A4 lineage among young cervical cancer patients, which was the unique HPV16 E6 variant found in Chinese young patients. Our finding may provide an insight into the pathogenic factors that associated with cervical cancer in young women. FAU - Hu, Yan AU - Hu Y AD - Department of Gynecology, 1 st Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, P.R.China. FAU - Wu, Jin-Ze AU - Wu JZ AD - Department of Anesthesiology, 1 st Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, P.R.China. FAU - Zhu, Hua AU - Zhu H AD - Department of Gynecology, 1 st Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, P.R.China. FAU - Zhang, Sheng-Hui AU - Zhang SH AD - Department of Hematology, 1 st Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, P.R.China. FAU - Zhu, Yan-Ying AU - Zhu YY AD - Laboratory Diagnosis Center, 1 st Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, P.R.China. FAU - Wu, Yi-Yao AU - Wu YY AD - College of Medicine, Jiaxing University, Jiaxing, Zhejiang, P.R.China. FAU - Shuai, Ci-Xia AU - Shuai CX AD - Department of Gynecology, 1 st Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, P.R.China. LA - eng PT - Journal Article DEP - 20170722 PL - Australia TA - J Cancer JT - Journal of Cancer JID - 101535920 PMC - PMC5560159 OTO - NOTNLM OT - age OT - human leukocyte antigen OT - human papillomavirus OT - polymorphism OT - variant COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2017/08/19 06:00 MHDA- 2017/08/19 06:01 PMCR- 2017/01/01 CRDT- 2017/08/19 06:00 PHST- 2017/02/25 00:00 [received] PHST- 2017/05/14 00:00 [accepted] PHST- 2017/08/19 06:00 [entrez] PHST- 2017/08/19 06:00 [pubmed] PHST- 2017/08/19 06:01 [medline] PHST- 2017/01/01 00:00 [pmc-release] AID - jcav08p2401 [pii] AID - 10.7150/jca.19809 [doi] PST - epublish SO - J Cancer. 2017 Jul 22;8(12):2401-2409. doi: 10.7150/jca.19809. eCollection 2017.