PMID- 28822779
OWN - NLM
STAT- MEDLINE
DCOM- 20180309
LR  - 20180309
IS  - 1531-4332 (Electronic)
IS  - 1095-6433 (Linking)
VI  - 213
DP  - 2017 Nov
TI  - Molecular characterization of kiss2 and differential regulation of 
      reproduction-related genes by sex steroids in the hypothalamus of half-smooth 
      tongue sole (Cynoglossus semilaevis).
PG  - 46-55
LID - S1095-6433(17)30183-6 [pii]
LID - 10.1016/j.cbpa.2017.08.003 [doi]
AB  - Kisspeptin (Kiss) plays a critical role in mediating gonadal steroid feedback to 
      the gonadotropin-releasing hormone (GnRH) neurons in mammals. However, little 
      information regarding the regulation of kisspeptin gene by sex steroids is 
      available in teleosts. In this study, we examined the direct actions of estradiol 
      (E2) and testosterone (T) on hypothalamic expression of kisspeptin and other key 
      factors involved in reproductive function of half-smooth tongue sole. As a first 
      step, a partial-length cDNA of kiss2 was identified from the brain of tongue sole 
      and kiss2 transcript levels were shown to be widely expressed in various tissues, 
      notably in the ovary. Then, the actions of sex steroids on kiss2 and other 
      reproduction-related genes were evaluated using a primary hypothalamus culture 
      system. Our results showed that neither kiss2 nor its receptor kiss2r mRNA levels 
      were significantly altered by sex steroids. Moreover, sex steroids did not modify 
      hypothalamic expression of gonadotropin-inhibitory hormone (gnih) and its 
      receptor gnihr mRNAs, either. However, E2 markedly stimulated both gnrh2 and 
      gnrh3 mRNAs levels. Overall, this study provides insights into the role of sex 
      steroids in the reproductive function of Pleuronectiform teleosts.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Wang, Bin
AU  - Wang B
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries and Food 
      Production Processes, Qingdao National Laboratory for Marine Science and 
      Technology, Qingdao 266237, China.
FAU - Liu, Quan
AU  - Liu Q
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; College of Fisheries and Life Science, Shanghai 
      Ocean University, Shanghai 201306, China.
FAU - Liu, Xuezhou
AU  - Liu X
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries and Food 
      Production Processes, Qingdao National Laboratory for Marine Science and 
      Technology, Qingdao 266237, China. Electronic address: liuxz@ysfri.ac.cn.
FAU - Xu, Yongjiang
AU  - Xu Y
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries and Food 
      Production Processes, Qingdao National Laboratory for Marine Science and 
      Technology, Qingdao 266237, China.
FAU - Song, Xuesong
AU  - Song X
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; College of Fisheries and Life Science, Shanghai 
      Ocean University, Shanghai 201306, China.
FAU - Shi, Bao
AU  - Shi B
AD  - Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of 
      Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries and Food 
      Production Processes, Qingdao National Laboratory for Marine Science and 
      Technology, Qingdao 266237, China.
LA  - eng
PT  - Journal Article
DEP - 20170816
PL  - United States
TA  - Comp Biochem Physiol A Mol Integr Physiol
JT  - Comparative biochemistry and physiology. Part A, Molecular & integrative 
      physiology
JID - 9806096
RN  - 0 (DNA, Complementary)
RN  - 0 (Estrogens)
RN  - 0 (Kisspeptins)
RN  - 0 (RNA, Messenger)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Estrogens/*genetics
MH  - Female
MH  - Flatfishes/*physiology
MH  - *Gene Expression Regulation
MH  - Gonadotropin-Releasing Hormone/*genetics
MH  - Hypothalamus/*metabolism
MH  - Kisspeptins/*genetics
MH  - Phylogeny
MH  - RNA, Messenger/genetics
MH  - Reproduction/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Testosterone/*genetics
OTO - NOTNLM
OT  - Cynoglossus semilaevis
OT  - Gene expression
OT  - Kisspeptin
OT  - Sex steroids
EDAT- 2017/08/22 06:00
MHDA- 2018/03/10 06:00
CRDT- 2017/08/21 06:00
PHST- 2017/04/08 00:00 [received]
PHST- 2017/07/28 00:00 [revised]
PHST- 2017/08/14 00:00 [accepted]
PHST- 2017/08/22 06:00 [pubmed]
PHST- 2018/03/10 06:00 [medline]
PHST- 2017/08/21 06:00 [entrez]
AID - S1095-6433(17)30183-6 [pii]
AID - 10.1016/j.cbpa.2017.08.003 [doi]
PST - ppublish
SO  - Comp Biochem Physiol A Mol Integr Physiol. 2017 Nov;213:46-55. doi: 
      10.1016/j.cbpa.2017.08.003. Epub 2017 Aug 16.