PMID- 28826820
OWN - NLM
STAT- MEDLINE
DCOM- 20170925
LR  - 20181202
IS  - 1878-1551 (Electronic)
IS  - 1534-5807 (Linking)
VI  - 42
IP  - 5
DP  - 2017 Sep 11
TI  - Antagonistic Activities of Sox2 and Brachyury Control the Fate Choice of 
      Neuro-Mesodermal Progenitors.
PG  - 514-526.e7
LID - S1534-5807(17)30597-X [pii]
LID - 10.1016/j.devcel.2017.07.021 [doi]
AB  - The spinal cord and mesodermal tissues of the trunk such as the vertebral column 
      and skeletal musculature derive from neuro-mesodermal progenitors (NMPs). Sox2, 
      Brachyury (T), and Tbx6 have been correlated with NMP potency and lineage choice; 
      however, their exact role and interaction in these processes have not yet been 
      revealed. Here we present a global analysis of NMPs and their descending lineages 
      performed on purified cells from embryonic day 8.5 wild-type and mutant embryos. 
      We show that T, cooperatively with WNT signaling, controls the progenitor state 
      and the switch toward the mesodermal fate. Sox2 acts antagonistically and 
      promotes neural development. T is also involved in remodeling the chromatin for 
      mesodermal development. Tbx6 reinforces the mesodermal fate choice, represses the 
      progenitor state, and confers paraxial fate commitment. Our findings refine 
      previous models and establish molecular principles underlying mammalian trunk 
      development, comprising NMP maintenance, lineage choice, and mesoderm formation.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Koch, Frederic
AU  - Koch F
AD  - Department of Developmental Genetics, Max Planck Institute for Molecular 
      Genetics, Ihnestr. 63-73, 14195 Berlin, Germany.
FAU - Scholze, Manuela
AU  - Scholze M
AD  - Department of Developmental Genetics, Max Planck Institute for Molecular 
      Genetics, Ihnestr. 63-73, 14195 Berlin, Germany.
FAU - Wittler, Lars
AU  - Wittler L
AD  - Department of Developmental Genetics, Max Planck Institute for Molecular 
      Genetics, Ihnestr. 63-73, 14195 Berlin, Germany.
FAU - Schifferl, Dennis
AU  - Schifferl D
AD  - Department of Developmental Genetics, Max Planck Institute for Molecular 
      Genetics, Ihnestr. 63-73, 14195 Berlin, Germany.
FAU - Sudheer, Smita
AU  - Sudheer S
AD  - Department of Developmental Genetics, Max Planck Institute for Molecular 
      Genetics, Ihnestr. 63-73, 14195 Berlin, Germany.
FAU - Grote, Phillip
AU  - Grote P
AD  - Department of Developmental Genetics, Max Planck Institute for Molecular 
      Genetics, Ihnestr. 63-73, 14195 Berlin, Germany.
FAU - Timmermann, Bernd
AU  - Timmermann B
AD  - Sequencing Core Facility, Max Planck Institute for Molecular Genetics, Ihnestr. 
      63-73, 14195 Berlin, Germany.
FAU - Macura, Karol
AU  - Macura K
AD  - Department of Developmental Genetics, Max Planck Institute for Molecular 
      Genetics, Ihnestr. 63-73, 14195 Berlin, Germany.
FAU - Herrmann, Bernhard G
AU  - Herrmann BG
AD  - Department of Developmental Genetics, Max Planck Institute for Molecular 
      Genetics, Ihnestr. 63-73, 14195 Berlin, Germany; Institute for Medical Genetics, 
      Charite - University Medicine Berlin, Campus Benjamin Franklin, Hindenburgdamm 
      30, 12203 Berlin, Germany. Electronic address: herrmann@molgen.mpg.de.
LA  - eng
PT  - Journal Article
DEP - 20170817
PL  - United States
TA  - Dev Cell
JT  - Developmental cell
JID - 101120028
RN  - 0 (Chromatin)
RN  - 0 (Fetal Proteins)
RN  - 0 (SOXB1 Transcription Factors)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (Tbx6 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - EQ43SC3GDB (Brachyury protein)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Lineage/*genetics
MH  - Chromatin/metabolism
MH  - Chromatin Assembly and Disassembly/genetics
MH  - Fetal Proteins/genetics/*metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental
MH  - Mesoderm/*cytology
MH  - Mice
MH  - Models, Biological
MH  - Neurons/*cytology/metabolism
MH  - SOXB1 Transcription Factors/genetics/*metabolism
MH  - Single-Cell Analysis
MH  - Stem Cells/*cytology/metabolism
MH  - T-Box Domain Proteins/genetics/*metabolism
MH  - Transcription Factors/metabolism
MH  - Wnt Signaling Pathway/genetics
OTO - NOTNLM
OT  - NMP
OT  - chromatin
OT  - lineage choice
OT  - mesoderm
OT  - mouse
OT  - neuro-mesodermal progenitors
OT  - neuroectoderm
OT  - single-cell analysis
OT  - transcriptome
OT  - trunk development
EDAT- 2017/08/23 06:00
MHDA- 2017/09/26 06:00
CRDT- 2017/08/23 06:00
PHST- 2017/01/26 00:00 [received]
PHST- 2017/06/07 00:00 [revised]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/08/23 06:00 [pubmed]
PHST- 2017/09/26 06:00 [medline]
PHST- 2017/08/23 06:00 [entrez]
AID - S1534-5807(17)30597-X [pii]
AID - 10.1016/j.devcel.2017.07.021 [doi]
PST - ppublish
SO  - Dev Cell. 2017 Sep 11;42(5):514-526.e7. doi: 10.1016/j.devcel.2017.07.021. Epub 
      2017 Aug 17.