PMID- 28828737
OWN - NLM
STAT- MEDLINE
DCOM- 20180725
LR  - 20181202
IS  - 1530-9932 (Electronic)
IS  - 1530-9932 (Linking)
VI  - 19
IP  - 1
DP  - 2018 Jan
TI  - Cationic Niosomes for Enhanced Skin Immunization of Plasmid DNA-Encoding 
      Ovalbumin via Hollow Microneedles.
PG  - 481-488
LID - 10.1208/s12249-017-0855-5 [doi]
AB  - The purpose of the present study was to evaluate the use of cationic niosomes 
      composed of Span20:cholesterol:cationic lipid (N (1),N 
      (1)-dimyristeroyloxyethyl-spermine) at the molar ratio of 2.5:2.5:0.5 mM combined 
      with hollow microneedle (MN) devices for in vivo skin immunization of plasmid 
      DNA-encoding ovalbumin (pOVA). The results revealed that using hollow MNs with 
      cationic niosomes for pOVA penetration successfully induced both humoral and 
      cell-mediated immune responses including immunoglobulin G (IgG) antibody 
      responses, interleukin-4 (IL-4), and interferon gamma (IFN-gamma) cytokine secretion. 
      When using hollow MNs with cationic niosome/pOVA complexes, the immune response 
      was superior to naked pOVA, which testifies the increased amount of IgG antibody 
      responses and cytokine secretion. In comparison with conventional subcutaneous 
      (SC) injections, using hollow MNs with cationic niosome/pOVA complexes induced a 
      higher level of both IgG immune response and cytokine release. Moreover, a group 
      of mice immunized with hollow MNs did not show infection or bleeding on the skin. 
      Consequently, targeted delivery of pOVA using cationic niosomes combined with 
      hollow MNs might prove a promising vaccination method for skin vaccination.
FAU - Pamornpathomkul, Boonnada
AU  - Pamornpathomkul B
AD  - Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group 
      (PDGIG), Silpakorn University, Nakhon Pathom, 73000, Thailand.
FAU - Niyomtham, Nattisa
AU  - Niyomtham N
AD  - Faculty of Science, Department of Chemistry and Center of Excellence for 
      Innovation in Chemistry, Ramkhamhaeng University, Bangkok, Thailand.
FAU - Yingyongnarongkul, Boon-Ek
AU  - Yingyongnarongkul BE
AD  - Faculty of Science, Department of Chemistry and Center of Excellence for 
      Innovation in Chemistry, Ramkhamhaeng University, Bangkok, Thailand.
FAU - Prasitpuriprecha, Chutinun
AU  - Prasitpuriprecha C
AD  - Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon 
      Ratchathani, Thailand.
FAU - Rojanarata, Theerasak
AU  - Rojanarata T
AD  - Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group 
      (PDGIG), Silpakorn University, Nakhon Pathom, 73000, Thailand.
FAU - Ngawhirunpat, Tanasait
AU  - Ngawhirunpat T
AD  - Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group 
      (PDGIG), Silpakorn University, Nakhon Pathom, 73000, Thailand.
FAU - Opanasopit, Praneet
AU  - Opanasopit P
AD  - Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group 
      (PDGIG), Silpakorn University, Nakhon Pathom, 73000, Thailand. 
      opanasopit_p@su.ac.th.
LA  - eng
PT  - Journal Article
DEP - 20170821
PL  - United States
TA  - AAPS PharmSciTech
JT  - AAPS PharmSciTech
JID - 100960111
RN  - 0 (Cations)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Liposomes)
RN  - 0 (Vaccines, DNA)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Cations
MH  - Cytokines/metabolism
MH  - Female
MH  - Immunoglobulin G/immunology
MH  - Injections, Subcutaneous
MH  - Liposomes/*chemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microinjections
MH  - Needles
MH  - *Ovalbumin
MH  - Plasmids/immunology
MH  - Skin/immunology
MH  - Vaccination/adverse effects/*methods
MH  - Vaccines, DNA/*administration & dosage/adverse effects
OTO - NOTNLM
OT  - Cationic niosomes
OT  - Hollow microneedle
OT  - Nanocarriers
OT  - Plasmid ovalbumin
OT  - Skin immunization
EDAT- 2017/08/23 06:00
MHDA- 2018/07/26 06:00
CRDT- 2017/08/23 06:00
PHST- 2017/07/01 00:00 [received]
PHST- 2017/08/06 00:00 [accepted]
PHST- 2017/08/23 06:00 [pubmed]
PHST- 2018/07/26 06:00 [medline]
PHST- 2017/08/23 06:00 [entrez]
AID - 10.1208/s12249-017-0855-5 [pii]
AID - 10.1208/s12249-017-0855-5 [doi]
PST - ppublish
SO  - AAPS PharmSciTech. 2018 Jan;19(1):481-488. doi: 10.1208/s12249-017-0855-5. Epub 
      2017 Aug 21.