PMID- 28830563 OWN - NLM STAT- MEDLINE DCOM- 20180409 LR - 20181113 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 14 IP - 1 DP - 2017 Aug 22 TI - ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro. PG - 159 LID - 10.1186/s12985-017-0825-2 [doi] LID - 159 AB - BACKGROUND: Currently, porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important viral pathogens in swine in most countries, especially China. Two PRRSV attenuated live vaccine strains (HuN4-F112 and CH-1R) are currently widely used in China. Our previous study showed that HuN4-F112, but not CH-1R, induced high anti-nucleocapsid (N) antibody and neutralizing antibody (NA) titers. Additionally, sera from HuN4-F112 inoculated pigs induced low cross neutralization of CH-1R. METHODS: In the present study, 6 chimeric viruses through exchanging 5' untranslated region (UTR) + open reading frame (ORF)1a, ORF1b, and ORF2-7 + 3'UTR between HuN4-F112 and CH-1R were constructed and rescued based on the infectious clones of rHuN4-F112 and rCH-1R. The characteristics of these viruses were investigated in vitro and vivo. RESULTS: All the three fragments, 5'UTR + ORF1a, ORF1b, and ORF2-7 + 3'UTR, could affect the replication efficiencies of rHuN4-F112 and rCH-1R in vitro. Additionally, both 5'UTR + ORF1a and ORF2-7 + 3'UTR affected the anti-N antibody and NA responses targeting rHuN4-F112 and rCH-1R in piglets. CONCLUSIONS: The 5'UTR + ORF1a region of HuN4-F112 played a key role in inducing NAs in piglets. Furthermore, we confirmed for the first time that ORF1a contains a neutralization region. This study provides important information that can be used for further study of the generation of anti-PRRSV NAs. FAU - Leng, Chaoliang AU - Leng C AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. AD - Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473061, China. FAU - Zhang, Wuchao AU - Zhang W AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. FAU - Zhang, Hongliang AU - Zhang H AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. FAU - Kan, Yunchao AU - Kan Y AD - Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473061, China. FAU - Yao, Lunguang AU - Yao L AD - Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473061, China. FAU - Zhai, Hongyue AU - Zhai H AD - Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473061, China. FAU - Li, Mingliang AU - Li M AD - Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, 473061, China. FAU - Li, Zhen AU - Li Z AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. FAU - Liu, Chunxiao AU - Liu C AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. FAU - An, Tongqing AU - An T AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. FAU - Peng, Jinmei AU - Peng J AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. FAU - Wang, Qian AU - Wang Q AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. FAU - Leng, Yumin AU - Leng Y AD - College of Physics and Electronic Engineering, Nanyang Normal University, Nanyang, 473061, China. FAU - Cai, Xuehui AU - Cai X AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. FAU - Tian, Zhijun AU - Tian Z AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. tzj@hvri.ac.cn. FAU - Tong, Guangzhi AU - Tong G AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin, 150001, China. gztong@shvri.ac.cn. AD - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Minhang District, Shanghai, 200241, China. gztong@shvri.ac.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170822 PL - England TA - Virol J JT - Virology journal JID - 101231645 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (DNA, Complementary) RN - 0 (DNA, Viral) RN - 0 (Vaccines, Attenuated) RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Antibodies, Neutralizing/*immunology MH - Antibodies, Viral/blood/*immunology MH - Base Sequence MH - Cell Line MH - China MH - DNA, Complementary MH - DNA, Viral MH - Kinetics MH - Open Reading Frames/genetics/*immunology MH - Porcine Reproductive and Respiratory Syndrome/*immunology/*prevention & control MH - Porcine respiratory and reproductive syndrome virus/genetics/growth & development/*immunology/pathogenicity MH - Swine/immunology MH - Vaccination MH - Vaccines, Attenuated/genetics/isolation & purification/*therapeutic use MH - Viral Vaccines/immunology MH - Viremia/virology PMC - PMC5568364 OTO - NOTNLM OT - Chimeric virus OT - Neutralization region OT - Neutralizing antibody OT - PRRSV COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: The study was approved by the Animal Ethics Committee of School of Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences and performed in accordance with animal ethics guidelines and approved protocols. The Animal Ethics Committee approval number was SYXK (Hei) 2,011,022. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2017/08/24 06:00 MHDA- 2018/04/10 06:00 PMCR- 2017/08/22 CRDT- 2017/08/24 06:00 PHST- 2017/04/26 00:00 [received] PHST- 2017/08/14 00:00 [accepted] PHST- 2017/08/24 06:00 [entrez] PHST- 2017/08/24 06:00 [pubmed] PHST- 2018/04/10 06:00 [medline] PHST- 2017/08/22 00:00 [pmc-release] AID - 10.1186/s12985-017-0825-2 [pii] AID - 825 [pii] AID - 10.1186/s12985-017-0825-2 [doi] PST - epublish SO - Virol J. 2017 Aug 22;14(1):159. doi: 10.1186/s12985-017-0825-2.