PMID- 28831024
OWN - NLM
STAT- MEDLINE
DCOM- 20180608
LR  - 20200914
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 37
IP  - 5
DP  - 2017 Oct 31
TI  - Effect of Liuweibuqi capsules on the balance between MMP-9 and TIMP1 and 
      viability of alveolar macrophages in COPD.
LID - BSR20170880 [pii]
LID - 10.1042/BSR20170880 [doi]
AB  - The present study aims to investigate the effect of Liuweibuqi (LWBQ) capsules on 
      the expression of matrix metalloproteinase (MMP)-9 and TIMP1 and cell viability 
      of alveolar macrophages (AMs) in chronic obstructive pulmonary disease (COPD). 
      Rats were randomly divided into normal control (NC) group, model control (MC) 
      group, Jinshuibao (JSB) group, spleen aminopeptidase (PAT) group, and low dose of 
      LWBQ (LWBQ low), mid dose of LWBQ (LWBQ mid), and high dose of LWBQ (LWBQ high) 
      group (n=10). Lung function was measured with a spirometer. Serum cytokines 
      including tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) were detected 
      using ELISA. The expressions of MMP-9 and TIMP1 were detected by quantitative 
      real-time PCR (qRT-PCR) and Western blot. MTT assay and flow cytometry were used 
      to measure cell viability and apoptosis. Compared with the NC group, body weight 
      and lung function were reduced in the MC group. In addition, the serum levels of 
      IL-6 and TNF-alpha were higher in the MC group than those in the NC group. The 
      expression of MMP-9 protein in the AMs from rats was higher, and TIMP1 protein 
      was lower in the MC group compared with the NC group. After LWBQ capsules 
      treatment, compared with the MC group, the expression of inflammatory cytokines 
      and MMP-9 were lower and TIMP1 was higher. Moreover, after LWBQ-medicated serum 
      treatment, the release of inflammatory cytokines was reduced from AMs. Besides, 
      LWBQ-medicated serum decreased the expression of MMP-9 and increased the 
      expression of TIMP1 and cell viability compared with those in MC group. In 
      conclusion, LWBQ capsules can inhibit the release of inflammatory cytokines, 
      promote cell viability in AMs, and regulate the expression of MMP-9 and TIMP1.
CI  - (c) 2017 The Author(s).
FAU - Wang, Chengyang
AU  - Wang C
AD  - Department of Traditional Chinese Medicine, The First Affiliated Hospital, Anhui 
      Medical University, Hefei 230022, China.
FAU - Ding, Huanzhang
AU  - Ding H
AD  - Graduate School, Anhui University of Chinese Medicine, Hefei 230022, China.
FAU - Tang, Xiao
AU  - Tang X
AD  - Graduate School, Anhui University of Chinese Medicine, Hefei 230022, China.
FAU - Li, Zegeng
AU  - Li Z
AD  - Anhui Academy of Chinese Medicine, Hefei 230022, China zegeng_li@sohu.com.
FAU - Gan, Lei
AU  - Gan L
AD  - Department of Traditional Chinese Medicine, The First Affiliated Hospital, Anhui 
      Medical University, Hefei 230022, China.
LA  - eng
PT  - Journal Article
DEP - 20170919
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (TIMP1 protein, rat)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology/*therapeutic use
MH  - Cell Survival/*drug effects
MH  - Cytokines/analysis
MH  - Drugs, Chinese Herbal/pharmacology/*therapeutic use
MH  - Macrophages, Alveolar/*drug effects/pathology
MH  - Male
MH  - Matrix Metalloproteinase 9/*analysis
MH  - Pulmonary Disease, Chronic Obstructive/*drug therapy/pathology
MH  - Rats, Sprague-Dawley
MH  - Tissue Inhibitor of Metalloproteinase-1/*analysis
PMC - PMC5603752
OTO - NOTNLM
OT  - Liuweibuqi capsules
OT  - MMP-9
OT  - TIMP1
OT  - chronic obstructive pulmonary disease
OT  - inflammatory cytokines
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2017/08/24 06:00
MHDA- 2018/06/09 06:00
PMCR- 2017/09/19
CRDT- 2017/08/24 06:00
PHST- 2017/06/01 00:00 [received]
PHST- 2017/08/21 00:00 [revised]
PHST- 2017/08/21 00:00 [accepted]
PHST- 2017/08/24 06:00 [pubmed]
PHST- 2018/06/09 06:00 [medline]
PHST- 2017/08/24 06:00 [entrez]
PHST- 2017/09/19 00:00 [pmc-release]
AID - BSR20170880 [pii]
AID - 10.1042/BSR20170880 [doi]
PST - epublish
SO  - Biosci Rep. 2017 Sep 19;37(5):BSR20170880. doi: 10.1042/BSR20170880. Print 2017 
      Oct 31.