PMID- 28833699
OWN - NLM
STAT- MEDLINE
DCOM- 20180801
LR  - 20220330
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Print)
IS  - 0909-8836 (Linking)
VI  - 125
IP  - 5
DP  - 2017 Oct
TI  - Impaired polymorphonuclear neutrophils in the oral cavity of edentulous 
      individuals.
PG  - 371-378
LID - 10.1111/eos.12367 [doi]
AB  - Oral health is characterized by functional oral polymorphonuclear neutrophils 
      (oPMNs). Edentulism might be associated with a loss of oPMNs because these cells 
      enter the oral cavity primarily through the gingival crevices. The main aim of 
      this study was to investigate the numbers of oPMNs in rinse samples obtained from 
      edentulous (n = 21) and dentate (n = 20) subjects. A second study aim was to 
      investigate possible differences between oPMNs and peripheral blood 
      polymorphonuclear neutrophils (cPMNs). Apoptosis/necrosis and cell-activation 
      markers (CD11b, CD63 and CD66b) were analyzed using flow cytometry. Reactive 
      oxygen species (ROS) production was determined either without stimulation 
      (constitutive) or in response to 10 muM phorbol myristate acetate or Fusobacterium 
      nucleatum. The edentulous subjects presented with lower oPMN counts and higher 
      percentages of apoptotic/necrotic oPMNs compared with dentate subjects. 
      Furthermore, oPMNs from edentulous donors expressed low levels of all three 
      activation markers and low constitutive ROS. In contrast, oPMNs from dentate 
      subjects expressed high levels of all three activation markers and a higher level 
      of constitutive ROS than cPMNs. When challenged, oPMNs from edentulous subjects 
      showed no upregulation in ROS production, whereas oPMNs from dentate subjects 
      retained their ability to respond to stimulation. The functional characteristics 
      of cPMNs were comparable between edentulous and dentate subjects. This study 
      demonstrates that despite having functional cPMNs, edentulous subjects have low 
      oPMN numbers that are functionally impaired.
CI  - (c) 2017 The Authors. Eur J Oral Sci published by John Wiley & Sons Ltd.
FAU - Rijkschroeff, Patrick
AU  - Rijkschroeff P
AUID- ORCID: 0000-0002-3997-0878
AD  - Department of Periodontology, Academic Centre for Dentistry Amsterdam, Amsterdam, 
      the Netherlands.
FAU - Loos, Bruno G
AU  - Loos BG
AD  - Department of Periodontology, Academic Centre for Dentistry Amsterdam, Amsterdam, 
      the Netherlands.
FAU - Nicu, Elena A
AU  - Nicu EA
AD  - Department of Periodontology, Academic Centre for Dentistry Amsterdam, Amsterdam, 
      the Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170819
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Antigens, CD)
RN  - 0 (CD11b Antigen)
RN  - 0 (CD63 protein, human)
RN  - 0 (CEACAM8 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (ITGAM protein, human)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tetraspanin 30)
SB  - IM
MH  - Aged
MH  - Antigens, CD/metabolism
MH  - Apoptosis
MH  - CD11b Antigen/metabolism
MH  - Case-Control Studies
MH  - Cell Adhesion Molecules/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - GPI-Linked Proteins/metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Mouth, Edentulous/*metabolism
MH  - Necrosis
MH  - Neutrophils/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Tetraspanin 30/metabolism
PMC - PMC5601278
OTO - NOTNLM
OT  - apoptosis
OT  - granulocytes
OT  - innate immunity
OT  - oral health
OT  - reactive oxygen species
EDAT- 2017/08/24 06:00
MHDA- 2018/08/02 06:00
PMCR- 2017/09/18
CRDT- 2017/08/24 06:00
PHST- 2017/06/28 00:00 [accepted]
PHST- 2017/08/24 06:00 [pubmed]
PHST- 2018/08/02 06:00 [medline]
PHST- 2017/08/24 06:00 [entrez]
PHST- 2017/09/18 00:00 [pmc-release]
AID - EOS12367 [pii]
AID - 10.1111/eos.12367 [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2017 Oct;125(5):371-378. doi: 10.1111/eos.12367. Epub 2017 Aug 
      19.