PMID- 28833875
OWN - NLM
STAT- MEDLINE
DCOM- 20180306
LR  - 20240109
IS  - 1096-9888 (Electronic)
IS  - 1076-5174 (Linking)
VI  - 52
IP  - 12
DP  - 2017 Dec
TI  - Characterization of phenolic compounds in green and red oak-leaf lettuce 
      cultivars by UHPLC-DAD-ESI-QToF/MS using MS(E) scan mode.
PG  - 873-902
LID - 10.1002/jms.4021 [doi]
AB  - Lettuce (Lactuca sativa) is one of the most popular leafy vegetables in the world 
      and constitutes a major dietary source of phenolic compounds with 
      health-promoting properties. In particular, the demand for green and red oak-leaf 
      lettuces has considerably increased in the last years but few data on their 
      polyphenol composition are available. Moreover, the usage of analytical edge 
      technology can provide new structural information and allow the identification of 
      unknown polyphenols. In the present study, the phenolic profiles of green and red 
      oak-leaf lettuce cultivars were exhaustively characterized by 
      ultrahigh-performance liquid chromatography (UHPLC) coupled online to diode array 
      detection (DAD), electrospray ionization (ESI), and quadrupole time-of-flight 
      mass spectrometry (QToF/MS), using the MS(E) instrument acquisition mode for 
      recording simultaneously exact masses of precursor and fragment ions. One hundred 
      fifteen phenolic compounds were identified in the acidified hydromethanolic 
      extract of freeze-dried lettuce leaves. Forty-eight of these compounds were 
      tentatively identified for the first time in lettuce, and only 20 of them have 
      been previously reported in oak-leaf lettuce cultivars in literature. Both 
      oak-leaf lettuce cultivars presented similar phenolic composition, except for 
      apigenin-glucuronide and dihydroxybenzoic acid, only detected in the green 
      cultivar; and for luteolin-hydroxymalonylhexoside, an apigenin conjugate with 
      molecular formula C(40) H(54) O(19) (monoisotopic MW = 838.3259 u), 
      cyanidin-3-O-glucoside, cyanidin-3-O-(3''-O-malonyl)glucoside, 
      cyanidin-3-O-(6''-O-malonyl)glucoside, and cyanidin-3-O-(6''-O-acetyl)glucoside, 
      only found in the red cultivar. The UHPLC-DAD-ESI-QToF/MS(E) approach 
      demonstrated to be a useful tool for the characterization of phenolic compounds 
      in complex plant matrices.
CI  - Copyright (c) 2017 John Wiley & Sons, Ltd.
FAU - Viacava, Gabriela E
AU  - Viacava GE
AD  - Grupo de Investigacion en Ingenieria en Alimentos, CONICET, Departamento de 
      Ingenieria Quimica y en Alimentos, Facultad de Ingenieria, Universidad Nacional 
      de Mar del Plata, Juan B. Justo 4302, 7600, Mar del Plata, Argentina.
FAU - Roura, Sara I
AU  - Roura SI
AD  - Grupo de Investigacion en Ingenieria en Alimentos, CONICET, Departamento de 
      Ingenieria Quimica y en Alimentos, Facultad de Ingenieria, Universidad Nacional 
      de Mar del Plata, Juan B. Justo 4302, 7600, Mar del Plata, Argentina.
FAU - Berrueta, Luis A
AU  - Berrueta LA
AD  - Grupo de Investigacion de Quimica Analitica de Productos Naturales, Departamento 
      de Quimica Analitica, Facultad de Ciencia y Tecnologia, Universidad del Pais 
      Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), P.O. Box 644, 48080, Bilbao, 
      Spain.
FAU - Iriondo, Carmen
AU  - Iriondo C
AD  - Grupo de Investigacion de Quimica Analitica de Productos Naturales, Departamento 
      de Quimica Analitica, Facultad de Ciencia y Tecnologia, Universidad del Pais 
      Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), P.O. Box 644, 48080, Bilbao, 
      Spain.
FAU - Gallo, Blanca
AU  - Gallo B
AD  - Grupo de Investigacion de Quimica Analitica de Productos Naturales, Departamento 
      de Quimica Analitica, Facultad de Ciencia y Tecnologia, Universidad del Pais 
      Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), P.O. Box 644, 48080, Bilbao, 
      Spain.
FAU - Alonso-Salces, Rosa M
AU  - Alonso-Salces RM
AUID- ORCID: 0000-0003-2096-4125
AD  - Departamento de Biologia, CONICET, Facultad de Ciencias Exactas y Naturales, 
      Universidad Nacional de Mar del Plata, Funes 3350, 7600, Mar del Plata, 
      Argentina.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Mass Spectrom
JT  - Journal of mass spectrometry : JMS
JID - 9504818
RN  - 0 (Glucosides)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 7V515PI7F6 (Apigenin)
RN  - KUX1ZNC9J2 (Luteolin)
SB  - IM
MH  - Apigenin/chemistry
MH  - Chromatography, High Pressure Liquid/methods
MH  - Glucosides/chemistry
MH  - Lactuca/*chemistry
MH  - Luteolin/chemistry
MH  - Molecular Structure
MH  - Phenols/*analysis/chemistry
MH  - Plant Extracts/chemistry
MH  - Plant Leaves/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
OTO - NOTNLM
OT  - Lactuca sativa
OT  - anthocyanin
OT  - coumarin
OT  - lignan
OT  - mass spectrometry
OT  - polyphenol
EDAT- 2017/08/24 06:00
MHDA- 2018/03/07 06:00
CRDT- 2017/08/24 06:00
PHST- 2017/04/24 00:00 [received]
PHST- 2017/07/20 00:00 [revised]
PHST- 2017/08/17 00:00 [accepted]
PHST- 2017/08/24 06:00 [pubmed]
PHST- 2018/03/07 06:00 [medline]
PHST- 2017/08/24 06:00 [entrez]
AID - 10.1002/jms.4021 [doi]
PST - ppublish
SO  - J Mass Spectrom. 2017 Dec;52(12):873-902. doi: 10.1002/jms.4021.