PMID- 28835030 OWN - NLM STAT- MEDLINE DCOM- 20190130 LR - 20190130 IS - 1002-0098 (Print) IS - 1002-0098 (Linking) VI - 52 IP - 8 DP - 2017 Aug 9 TI - [Effect of dentin proteoglycans on the stability of resin-dentin bonds against artificial saliva storage]. PG - 486-491 LID - 10.3760/cma.j.issn.1002-0098.2017.08.008 [doi] AB - Objective: To investigate the potential effect of proteoglycans (PG) and glycosaminoglycans (GAG) on the stability of resin-dentin bonds against artificial saliva storage. Methods: Seventy-two extracted molars were used to obtain standard dentin bonding surface, and the specimens were etched for 15 s with 37% phosphoric acid and divided into three groups using a table of random number. Then the three groups undergone different incubating procedures as follow: specimens in chondroitinase ABC (C-ABC) group were incubated with C-ABC, specimens in trypsin (TRY) group were incubated with trypsin, and specimens in the control group were incubated with deionized water. All specimens were incubated at 37 ℃ for 48 h in the oscillators. Then specimens in each group were randomly assigned into three subgroups (n=8) as follows: immediate control subgroup, aging subgroups with artificial saliva storage for 6 months and 12 months. Microtensile bond strength (muTBS), fracture mode, bonding interface morphology and nanoleakage were evaluated. Results: Immediately and with artificial saliva storage for 6 months and 12 mouths, the muTBS of TRY group ([49.04+/-3.57], [37.01+/-3.21] and [35.27+/-3.56] MPa) were significantly higher than those in the control group ([40.71+/-3.32], [28.87+/-2.34] and [24.20+/-2.07] MPa) (P<0.05). The immediate muTBS of C-ABC group ([32.94+/-2.45] MPa) was significantly lower than that of the control group (P<0.05). While with artificial saliva storage for 6 months and 12 mouths, the muTBS of C-ABC group ([26.46+/-2.45] and [22.50+/-2.58] MPa) were no differences with those of the control group (P>0.05). The ratio of cohesive fracture increased with the extension of aging time. Some narrow gaps were found in hybrid layer of the control group with artificial saliva storage for 6 months and 12 mouths. Conclusions: Removal of PG increased the muTBS and durable bonds to dentin, while removal of GAG decreased the muTBS, however, it can be of help to create more durable bonds to dentin. FAU - Gao, Y AU - Gao Y AD - Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University& State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi'an 710032, China(Present address: Department of Pediatric Dentistry, School of Stomatology, Shandong University & Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China). FAU - Zhao, S J AU - Zhao SJ AD - Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University& State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi'an 710032, China. FAU - Wang, P H AU - Wang PH AD - Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University& State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi'an 710032, China(Present address: Department of Stomatology, The General Hospital of Jinan Military Command, Jinan 250031, China). FAU - Lu, S AU - Lu S AD - Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University& State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi'an 710032, China(Present address: Department of Stomatology, The Official Hospital of Chengdu Military Command, Chengdu 610011, China). FAU - Chen, J H AU - Chen JH AD - Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University& State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Xi'an 710032, China. LA - chi PT - Journal Article PL - China TA - Zhonghua Kou Qiang Yi Xue Za Zhi JT - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology JID - 8711066 RN - 0 (Dental Materials) RN - 0 (Dentin-Bonding Agents) RN - 0 (Glycosaminoglycans) RN - 0 (Phosphoric Acids) RN - 0 (Proteoglycans) RN - 0 (Resin Cements) RN - 0 (Resins, Synthetic) RN - 0 (Saliva, Artificial) RN - E4GA8884NN (phosphoric acid) SB - IM MH - *Dental Bonding MH - Dental Etching MH - Dental Materials MH - Dentin/*chemistry MH - Dentin-Bonding Agents MH - Glycosaminoglycans/*pharmacology MH - Materials Testing MH - Molar MH - Phosphoric Acids MH - Proteoglycans/*pharmacology MH - Random Allocation MH - Resin Cements MH - Resins, Synthetic MH - *Saliva, Artificial MH - Tensile Strength/drug effects OTO - NOTNLM OT - Dentin-bonding agents OT - Nanoleakage OT - Proteoglycans OT - Tensile strength EDAT- 2017/08/25 06:00 MHDA- 2019/01/31 06:00 CRDT- 2017/08/25 06:00 PHST- 2017/08/25 06:00 [entrez] PHST- 2017/08/25 06:00 [pubmed] PHST- 2019/01/31 06:00 [medline] AID - 10.3760/cma.j.issn.1002-0098.2017.08.008 [doi] PST - ppublish SO - Zhonghua Kou Qiang Yi Xue Za Zhi. 2017 Aug 9;52(8):486-491. doi: 10.3760/cma.j.issn.1002-0098.2017.08.008.