PMID- 28835082
OWN - NLM
STAT- MEDLINE
DCOM- 20190125
LR  - 20190125
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 39
IP  - 8
DP  - 2017 Aug 23
TI  - [Fluorescence in situ hybridization combined with cytomorphology for the 
      detection of lung cancer in bronchial brushing specimens].
PG  - 595-599
LID - 10.3760/cma.j.issn.0253-3766.2017.08.007 [doi]
AB  - Objective: To evaluate the diagnostic value of fluorescence in situ hybridization 
      (FISH) combined with bronchial brushing cytology for detecting lung cancer. 
      Methods: Centromeric enumeration probes (CEPs) for chromosomes 7, 8 and 17 were 
      used in FISH assay. The combination of FISH and cytology was analyzed in 69 
      bronchial brushing specimens. Results: The positive rates of CEP7, CEP8 and CEP17 
      in malignant cases diagnosed by cytology were 50.0%, 80.8% and 65.4%, 
      respectively. CEP8 probe showed significantly higher positive rate than CEP7 
      (P=0.015). In the samples of suspicious of malignancy, the positive rates of 
      CEP7, CEP8 and CEP17 were 46.6%, 66.7% and 58.8%, respectively. While in atypical 
      cases, the positive rates of these three probes were 20.0%, 33.3% and 25.0%, 
      respectively. There was no statistical difference between suspicious of 
      malignancy and atypical cases (P>0.05) as well as between malignant and 
      suspicious of malignancy (P>0.05). No chromosome aberrations were found in normal 
      cases diagnosed by cytology. The positive rates of these three probes in 
      adenocarcinoma (ADC) were slightly higher than those in squamous cell carcinoma 
      and small cell lung cancer. However, only CEP8 probe showed statistically 
      difference between ADC and small cell lung cancer (P=0.044). The combination of 
      cytology and FISH using any one of the three-probe set (CEP7, CEP8 and CEP17) 
      showed the sensitivity and specificity of 80.3% and 100.0%, while those of 
      cytology were 54.1% and 100.0%, respectively. Conclusions: FISH combined with 
      cytomorphology assisted the cytology diagnosis of suspicious of malignancy and 
      atypical cases. Therefore, it significantly improved the diagnostic sensitivity 
      for lung cancer without sacrificing specificity.
FAU - Lu, S S
AU  - Lu SS
AD  - Department of pathology, Peking University People's Hospital, Beijing 100044, 
      China.
FAU - Pan, Q J
AU  - Pan QJ
AD  - Department of pathology, National Cancer Center / Cancer Hospital, Chinese 
      Academy of Medical Sciences and Peking Union Medical College, Beijing 100021.
FAU - Cao, J
AU  - Cao J
AD  - Department of pathology, National Cancer Center / Cancer Hospital, Chinese 
      Academy of Medical Sciences and Peking Union Medical College, Beijing 100021.
FAU - Xu, X
AU  - Xu X
AD  - State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer 
      Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 
      Beijing 100021.
FAU - Zhao, H
AU  - Zhao H
AD  - Department of pathology, National Cancer Center / Cancer Hospital, Chinese 
      Academy of Medical Sciences and Peking Union Medical College, Beijing 100021.
FAU - Shen, D H
AU  - Shen DH
AD  - Department of pathology, Peking University People's Hospital, Beijing 100044, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
SB  - IM
MH  - Adenocarcinoma/diagnosis/*pathology
MH  - Biopsy
MH  - Carcinoma, Squamous Cell/diagnosis/*pathology
MH  - Chromosome Aberrations
MH  - Cytodiagnosis/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lung Neoplasms/diagnosis/*pathology
MH  - Sensitivity and Specificity
MH  - Small Cell Lung Carcinoma/diagnosis/*pathology
OTO - NOTNLM
OT  - Bronchial brushing cytology
OT  - Diagnosis
OT  - Fluorescence in situ hybridization
OT  - Lung neoplasms
EDAT- 2017/08/25 06:00
MHDA- 2019/01/27 06:00
CRDT- 2017/08/25 06:00
PHST- 2017/08/25 06:00 [entrez]
PHST- 2017/08/25 06:00 [pubmed]
PHST- 2019/01/27 06:00 [medline]
AID - 10.3760/cma.j.issn.0253-3766.2017.08.007 [doi]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2017 Aug 23;39(8):595-599. doi: 
      10.3760/cma.j.issn.0253-3766.2017.08.007.