PMID- 28835358
OWN - NLM
STAT- MEDLINE
DCOM- 20180529
LR  - 20190118
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 24
IP  - 10
DP  - 2017 Oct
TI  - Combined Action of Human Commensal Bacteria and Amorphous Silica Nanoparticles on 
      the Viability and Immune Responses of Dendritic Cells.
LID - 10.1128/CVI.00178-17 [doi]
LID - e00178-17
AB  - Dendritic cells (DCs) regulate the host-microbe balance in the gut and skin, 
      tissues likely exposed to nanoparticles (NPs) present in drugs, food, and 
      cosmetics. We analyzed the viability and the activation of DCs incubated with 
      extracellular media (EMs) obtained from cultures of commensal bacteria 
      (Escherichia coli, Staphylococcus epidermidis) or pathogenic bacteria 
      (Pseudomonas aeruginosa, Staphylococcus aureus) in the presence of amorphous 
      silica nanoparticles (SiO(2) NPs). EMs and NPs synergistically increased the 
      levels of cytotoxicity and cytokine production, with different nanoparticle 
      dose-response characteristics being found, depending on the bacterial species. E. 
      coli and S. epidermidis EMs plus NPs at nontoxic doses stimulated the secretion 
      of interleukin-1beta (IL-1beta), IL-12, IL-10, and IL-6, while E. coli and S. 
      epidermidis EMs plus NPs at toxic doses stimulated the secretion of gamma 
      interferon (IFN-gamma), tumor necrosis factor alpha (TNF-alpha), IL-4, and IL-5. On the 
      contrary, S. aureus and P. aeruginosa EMs induced cytokines only when they were 
      combined with NPs at toxic concentrations. The induction of maturation markers 
      (CD86, CD80, CD83, intercellular adhesion molecule 1, and major 
      histocompatibility complex class II) by commensal bacteria but not by pathogenic 
      ones was improved in the presence of noncytotoxic SiO(2) NP doses. DCs 
      consistently supported the proliferation and differentiation of CD4(+) and CD8(+) 
      T cells secreting IFN-gamma and IL-17A. The synergistic induction of CD86 was due to 
      nonprotein molecules present in the EMs from all bacteria tested. At variance 
      with this finding, the synergistic induction of IL-1beta was prevalently mediated by 
      proteins in the case of E. coli EMs and by nonproteins in the case of S. 
      epidermidis EMs. A bacterial costimulus did not act on DCs after adsorption on 
      SiO(2) NPs but rather acted as an independent agonist. The inflammatory and 
      immune actions of DCs stimulated by commensal bacterial agonists might be altered 
      by the simultaneous exposure to engineered or environmental NPs.
CI  - Copyright (c) 2017 American Society for Microbiology.
FAU - Malachin, Giulia
AU  - Malachin G
AD  - Department of Biomedical Sciences, University of Padua, Padua, Italy.
FAU - Lubian, Elisa
AU  - Lubian E
AD  - Department of Chemical Sciences, University of Padua, Padua, Italy.
FAU - Mancin, Fabrizio
AU  - Mancin F
AD  - Department of Chemical Sciences, University of Padua, Padua, Italy.
FAU - Papini, Emanuele
AU  - Papini E
AD  - Department of Biomedical Sciences, University of Padua, Padua, Italy 
      emanuele.papini@unipd.it regina.tavano@unipd.it.
FAU - Tavano, Regina
AU  - Tavano R
AD  - Department of Biomedical Sciences, University of Padua, Padua, Italy 
      emanuele.papini@unipd.it regina.tavano@unipd.it.
LA  - eng
PT  - Journal Article
DEP - 20171005
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antigens, CD)
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
RN  - 0 (IL10 protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, CD/genetics
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/*drug effects
MH  - Culture Media/chemistry/*pharmacology
MH  - Cytokines/biosynthesis/immunology
MH  - Dendritic Cells/*drug effects/*immunology/physiology
MH  - Escherichia coli/drug effects/physiology
MH  - Humans
MH  - Interferon-gamma/immunology/metabolism
MH  - Interleukin-10/biosynthesis/immunology
MH  - Interleukin-1beta/immunology/metabolism
MH  - Interleukin-4/biosynthesis/immunology
MH  - Nanoparticles/*adverse effects/chemistry
MH  - Pseudomonas aeruginosa/drug effects/pathogenicity/physiology
MH  - Silicon Dioxide/*adverse effects/pharmacology
MH  - Staphylococcus aureus/drug effects/pathogenicity/physiology
MH  - Staphylococcus epidermidis/drug effects/physiology
MH  - *Symbiosis
PMC - PMC5629674
OTO - NOTNLM
OT  - DC maturation
OT  - commensal and pathogenic bacteria
OT  - cytokines
OT  - nanoparticle toxicity
EDAT- 2017/08/25 06:00
MHDA- 2018/05/31 06:00
PMCR- 2017/10/05
CRDT- 2017/08/25 06:00
PHST- 2017/06/06 00:00 [received]
PHST- 2017/08/13 00:00 [accepted]
PHST- 2017/08/25 06:00 [pubmed]
PHST- 2018/05/31 06:00 [medline]
PHST- 2017/08/25 06:00 [entrez]
PHST- 2017/10/05 00:00 [pmc-release]
AID - CVI.00178-17 [pii]
AID - 00178-17 [pii]
AID - 10.1128/CVI.00178-17 [doi]
PST - epublish
SO  - Clin Vaccine Immunol. 2017 Oct 5;24(10):e00178-17. doi: 10.1128/CVI.00178-17. 
      Print 2017 Oct.