PMID- 28836285
OWN - NLM
STAT- MEDLINE
DCOM- 20180820
LR  - 20180820
IS  - 1600-051X (Electronic)
IS  - 0303-6979 (Linking)
VI  - 44
IP  - 12
DP  - 2017 Dec
TI  - Periodontal treatment modulates gene expression of endothelial progenitor cells 
      in diabetic patients.
PG  - 1253-1263
LID - 10.1111/jcpe.12806 [doi]
AB  - BACKGROUND: Periodontitis is closely linked with type 2 diabetes mellitus (T2DM) 
      and endothelial dysfunction. This study investigated the effects of periodontal 
      treatment on immuno-inflammatory gene expression of endothelial progenitor cells 
      (EPCs) in diabetic patients. METHODS: Eighteen T2DM patients with moderate to 
      severe chronic periodontitis were randomly assigned to the Treatment group with 
      oral hygiene instruction (OHI), scaling and root debridement (n = 11), and 
      Control group (n = 7) with OHI alone. Peripheral blood samples were taken for 
      biochemical analysis and culture of EPCs at baseline and 6 months after the 
      treatment. PCR array-based profiling of 84 Toll-like receptor signalling-related 
      genes in EPCs was firstly assessed for four randomly selected patients from the 
      Treatment group. The differentially expressed genes were then further validated 
      by qPCR in both groups. RESULTS: All subjects in the Treatment group 
      significantly improved their periodontal conditions. Among the 84 genes tested, 
      IL-6 and IL-8 transcripts were significantly downregulated with over twofold 
      change after the treatment, and this observation was further validated by qPCR in 
      all subjects from both groups (p < .05). CONCLUSION: This preliminary study 
      suggests that periodontal treatment may contribute to a notable reduction in 
      immuno-inflammatory gene expression measured by IL-6 and IL-8 transcripts in 
      EPCs.
CI  - (c) 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Wang, Yi
AU  - Wang Y
AD  - Discipline of Periodontology, Faculty of Dentistry, The University of Hong Kong, 
      Hong Kong SAR, China.
FAU - Liu, Hin Nam
AU  - Liu HN
AD  - Discipline of Periodontology, Faculty of Dentistry, The University of Hong Kong, 
      Hong Kong SAR, China.
FAU - Zhen, Zhe
AU  - Zhen Z
AD  - Division of Cardiology, Department of Medicine, Li Ka Shing Faculty of Medicine, 
      The University of Hong Kong, Hong Kong SAR, China.
FAU - Yiu, Kai Hang
AU  - Yiu KH
AD  - Division of Cardiology, Department of Medicine, Li Ka Shing Faculty of Medicine, 
      The University of Hong Kong, Hong Kong SAR, China.
FAU - Tse, Hung Fat
AU  - Tse HF
AD  - Division of Cardiology, Department of Medicine, Li Ka Shing Faculty of Medicine, 
      The University of Hong Kong, Hong Kong SAR, China.
FAU - Pelekos, George
AU  - Pelekos G
AD  - Discipline of Periodontology, Faculty of Dentistry, The University of Hong Kong, 
      Hong Kong SAR, China.
FAU - Tonetti, Maurizio
AU  - Tonetti M
AD  - Discipline of Periodontology, Faculty of Dentistry, The University of Hong Kong, 
      Hong Kong SAR, China.
FAU - Jin, Lijian
AU  - Jin L
AUID- ORCID: 0000-0002-5340-9710
AD  - Discipline of Periodontology, Faculty of Dentistry, The University of Hong Kong, 
      Hong Kong SAR, China.
LA  - eng
PT  - Journal Article
DEP - 20171024
PL  - United States
TA  - J Clin Periodontol
JT  - Journal of clinical periodontology
JID - 0425123
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Cell Culture Techniques
MH  - Child
MH  - Chronic Periodontitis/blood/immunology/*therapy
MH  - Dental Plaque Index
MH  - Dental Scaling
MH  - Diabetes Mellitus, Type 2/blood/*complications
MH  - Endothelial Progenitor Cells/*metabolism
MH  - Female
MH  - *Gene Expression
MH  - Hong Kong
MH  - Humans
MH  - Inflammation/*genetics/immunology
MH  - Interleukin-6/blood/metabolism
MH  - Interleukin-8/blood/metabolism
MH  - Male
MH  - Middle Aged
MH  - Oral Hygiene
MH  - Periodontal Index
MH  - Periodontal Pocket/classification
MH  - Treatment Outcome
MH  - Young Adult
OTO - NOTNLM
OT  - endothelial progenitor cell
OT  - gene expression
OT  - inflammation
OT  - periodontitis
OT  - type 2 diabetes
EDAT- 2017/08/25 06:00
MHDA- 2018/08/21 06:00
CRDT- 2017/08/25 06:00
PHST- 2017/08/18 00:00 [accepted]
PHST- 2017/08/25 06:00 [pubmed]
PHST- 2018/08/21 06:00 [medline]
PHST- 2017/08/25 06:00 [entrez]
AID - 10.1111/jcpe.12806 [doi]
PST - ppublish
SO  - J Clin Periodontol. 2017 Dec;44(12):1253-1263. doi: 10.1111/jcpe.12806. Epub 2017 
      Oct 24.