PMID- 28836743
OWN - NLM
STAT- MEDLINE
DCOM- 20181109
LR  - 20181109
IS  - 1758-2229 (Electronic)
IS  - 1758-2229 (Linking)
VI  - 9
IP  - 6
DP  - 2017 Dec
TI  - Hydrography shapes community composition and diversity of amoA-containing 
      Thaumarchaeota in the coastal waters off central Chile.
PG  - 717-728
LID - 10.1111/1758-2229.12579 [doi]
AB  - Thaumarchaea are often abundant in low oxygen marine environments, and recent 
      kinetic studies indicate a capacity for aerobic ammonia oxidation at vanishingly 
      low oxygen levels (nM). However, molecular diversity surveys targeting this group 
      to high sequencing coverage are limited, and how these populations are coupled to 
      changes in dissolved oxygen remains unknown. In this study, the ammonia 
      monooxygenase subunit A (amoA) gene was sequenced from samples collected in the 
      Chilean coast (36.5  degrees S), a system prone to recurrent seasonal hypoxia and anoxia, 
      at several depths over one year, to read depths that saturated coverage 
      statistics. Temperature, salinity and depth displayed a stronger impact on 
      community composition than chemical and biological variables, such as dissolved 
      oxygen. The Nitrosopumilus water-column A clade (WCA) displayed high proportional 
      representation in all samples (42%-100% of all amoA OTUs). The two dominant WCA 
      OTUs displayed differences in their distributions that were inversely correlated 
      with one another, providing the first evidence for intra-subgroup specific 
      differences in the distributions among closely related WCA Thaumarcheota. 
      Nitrosopumilus water-column B (WCB) representatives displayed increased 
      proportional abundances (42%) at deeper depths during the spring and summer, were 
      highly coupled to decreased dissolved oxygen conditions and were non-detectable 
      during the austral winter. The depth of sequencing also enabled observation of 
      lower abundance taxa that are typically not observed in marine environments, such 
      as members of the genus Nitrosotalea amid austral winter surface waters. This 
      study highlights a strong coupling between Thaumarchaeal community diversity and 
      hydrographic variables, is the first to highlight intra-subclade depth specific 
      shifts in community diversity amongst members of the WCA clade, and links the WCB 
      clade to upwelling conditions associated with seasonal oxygen depletion.
CI  - (c) 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Bertagnolli, Anthony D
AU  - Bertagnolli AD
AD  - Department of Oceanography and Millennium Institute of Oceanography, Universidad 
      de Concepcion, Concepcion, P.O. Box 160 C, Chile.
FAU - Ulloa, Osvaldo
AU  - Ulloa O
AD  - Department of Oceanography and Millennium Institute of Oceanography, Universidad 
      de Concepcion, Concepcion, P.O. Box 160 C, Chile.
LA  - eng
PT  - Journal Article
DEP - 20171107
PL  - United States
TA  - Environ Microbiol Rep
JT  - Environmental microbiology reports
JID - 101499207
RN  - 0 (Archaeal Proteins)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Anaerobiosis
MH  - *Aquatic Organisms/classification/genetics
MH  - Archaea/*classification/enzymology/*genetics/metabolism
MH  - Archaeal Proteins/genetics
MH  - *Biodiversity
MH  - Chile
MH  - Kinetics
MH  - Oxidoreductases/*genetics
MH  - Phylogeny
MH  - Salinity
MH  - Seawater/*microbiology
MH  - Temperature
EDAT- 2017/08/25 06:00
MHDA- 2018/11/10 06:00
CRDT- 2017/08/25 06:00
PHST- 2017/03/20 00:00 [received]
PHST- 2017/08/11 00:00 [revised]
PHST- 2017/08/15 00:00 [accepted]
PHST- 2017/08/25 06:00 [pubmed]
PHST- 2018/11/10 06:00 [medline]
PHST- 2017/08/25 06:00 [entrez]
AID - 10.1111/1758-2229.12579 [doi]
PST - ppublish
SO  - Environ Microbiol Rep. 2017 Dec;9(6):717-728. doi: 10.1111/1758-2229.12579. Epub 
      2017 Nov 7.