PMID- 2883731
OWN - NLM
STAT- MEDLINE
DCOM- 19870624
LR  - 20190618
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 236
IP  - 4805
DP  - 1987 May 29
TI  - HTLV-I--associated B-cell CLL: indirect role for retrovirus in leukemogenesis.
PG  - 1103-6
AB  - Serum containing antibodies to the human T-lymphotropic virus type I (HTLV-I) has 
      been observed at a higher than expected frequency in patients with B-cell chronic 
      lymphocytic leukemia (CLL) in an area endemic for HTLV-I. An attempt was made to 
      determine whether the cells from patients with this leukemia were HTLV-I 
      antigen-committed B cells that had undergone malignant transformation. Cells from 
      two HTLV-I seropositive Jamaican patients with CLL were fused with a human 
      B-lymphoblastoid cell line. The hybridoma cells that resulted from the fusion of 
      CLL cells from patient I.C. produced an immunoglobulin (IgM) that reacted with 
      the p24 gag protein from HTLV-I, HTLV-II, and HTLV-III (now referred to as HIV), 
      but showed preferential reactivity with HTLV-I. The specific immunoglobulin gene 
      rearrangement (IgM, kappa) in the CLL cell was demonstrated in the hybridoma cell 
      line, indicating that the captured immunoglobulin was from the CLL cells. The IgM 
      secreted by the fusion of CLL cells from patient L.L. reacted only with 
      HTLV-I-infected cells and with the HTLV-I large envelope protein (gp61) on 
      Western blots. The CLL cells from these patients appear to be a malignant 
      transformation of an antigen-committed B cell responding to HTLV-I infection, 
      suggesting an indirect role for this retrovirus in leukemogenesis.
FAU - Mann, D L
AU  - Mann DL
FAU - DeSantis, P
AU  - DeSantis P
FAU - Mark, G
AU  - Mark G
FAU - Pfeifer, A
AU  - Pfeifer A
FAU - Newman, M
AU  - Newman M
FAU - Gibbs, N
AU  - Gibbs N
FAU - Popovic, M
AU  - Popovic M
FAU - Sarngadharan, M G
AU  - Sarngadharan MG
FAU - Gallo, R C
AU  - Gallo RC
FAU - Clark, J
AU  - Clark J
AU  - et al.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (HIV Antigens)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Antibodies, Viral/immunology
MH  - Antigens, Viral/immunology
MH  - B-Lymphocytes/microbiology
MH  - Deltaretrovirus/immunology
MH  - Deltaretrovirus Infections/*complications
MH  - HIV Antigens
MH  - Humans
MH  - Hybrid Cells/immunology
MH  - Immunoglobulin M/immunology
MH  - Leukemia, Lymphoid/etiology/*microbiology
MH  - T-Lymphocytes/immunology
EDAT- 1987/05/29 00:00
MHDA- 1987/05/29 00:01
CRDT- 1987/05/29 00:00
PHST- 1987/05/29 00:00 [pubmed]
PHST- 1987/05/29 00:01 [medline]
PHST- 1987/05/29 00:00 [entrez]
AID - 10.1126/science.2883731 [doi]
PST - ppublish
SO  - Science. 1987 May 29;236(4805):1103-6. doi: 10.1126/science.2883731.