PMID- 28842195
OWN - NLM
STAT- MEDLINE
DCOM- 20190903
LR  - 20190903
IS  - 1872-678X (Electronic)
IS  - 0165-0270 (Linking)
VI  - 300
DP  - 2018 Apr 15
TI  - Effects of a combination of 3,4-methylenedioxymeth amphetamine and caffeine on 
      real time stimulated dopamine release in the rat striatum: Studies using fast 
      cyclic voltammetry.
PG  - 216-223
LID - S0165-0270(17)30307-2 [pii]
LID - 10.1016/j.jneumeth.2017.08.026 [doi]
AB  - It is well documented that caffeine exacerbates the hyperthermia associated with 
      acute exposure to 3,4-methylenedioxymethamphetamine (MDMA) in rats. Previous 
      reports have also indicated that MDMA-related enhancement of dopamine release is 
      exacerbated in the presence of caffeine. In the present study we have examined 
      whether the effects of MDMA on real-time stimulated dopamine release, in the 
      absence of uptake inhibition, are accentuated in the presence of caffeine. 
      Isolated striatal slices from adult male Wistar rats were treated acutely with 
      MDMA, caffeine, or a combination, and their effects on single and 5pulse 
      stimulated dopamine release monitored using the technique of fast cyclic 
      voltammetry. Caffeine at 10 or 100muM had no significant effect on single pulse 
      stimulated dopamine release. However 100muM caffeine caused a significant peak 
      increase in 5pulse stimulated dopamine release. Both 1 and 30muM MDMA gave rise to 
      a significant increase in both single and 5-pulse dopamine release and reuptake. 
      A combination of 100muM caffeine and 1 or 30muM MDMA did not significantly enhance 
      the effects of MDMA on single or 5pulse dopamine release and reuptake when 
      compared to that applied alone. Utilizing single action potential dependent 
      dopamine release, these results do not demonstrate a caffeine-enhanced 
      MDMA-induced dopamine release.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - O'Connor, J J
AU  - O'Connor JJ
AD  - UCD School of Biomolecular & Biomedical Science, UCD Conway Institute, Belfield, 
      Dublin 4, Ireland; Department of Chemistry, Maynooth University, Maynooth, Co. 
      Kildare, Ireland. Electronic address: john.oconnor@ucd.ie.
FAU - O'Boyle, K M
AU  - O'Boyle KM
AD  - UCD School of Biomolecular & Biomedical Science, UCD Conway Institute, Belfield, 
      Dublin 4, Ireland; Department of Chemistry, Maynooth University, Maynooth, Co. 
      Kildare, Ireland.
FAU - Lowry, J P
AU  - Lowry JP
AD  - UCD School of Biomolecular & Biomedical Science, UCD Conway Institute, Belfield, 
      Dublin 4, Ireland; Department of Chemistry, Maynooth University, Maynooth, Co. 
      Kildare, Ireland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170824
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
RN  - 0 (Neurotransmitter Agents)
RN  - 3G6A5W338E (Caffeine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Caffeine/administration & dosage/*pharmacology
MH  - Chemistry Techniques, Analytical/*methods
MH  - Corpus Striatum/*drug effects
MH  - Dopamine/*metabolism
MH  - Drug Interactions
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/administration & dosage/*pharmacology
MH  - Neurotransmitter Agents/administration & dosage/*pharmacology
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Caffeine
OT  - Cyclic voltammetry
OT  - MDMA
OT  - Rat striatum
OT  - Real time dopamine release
OT  - Single pulse
EDAT- 2017/08/27 06:00
MHDA- 2019/09/04 06:00
CRDT- 2017/08/27 06:00
PHST- 2017/03/14 00:00 [received]
PHST- 2017/08/16 00:00 [revised]
PHST- 2017/08/17 00:00 [accepted]
PHST- 2017/08/27 06:00 [pubmed]
PHST- 2019/09/04 06:00 [medline]
PHST- 2017/08/27 06:00 [entrez]
AID - S0165-0270(17)30307-2 [pii]
AID - 10.1016/j.jneumeth.2017.08.026 [doi]
PST - ppublish
SO  - J Neurosci Methods. 2018 Apr 15;300:216-223. doi: 10.1016/j.jneumeth.2017.08.026. 
      Epub 2017 Aug 24.