PMID- 28842425
OWN - NLM
STAT- MEDLINE
DCOM- 20171211
LR  - 20240318
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Print)
IS  - 0892-6638 (Linking)
VI  - 31
IP  - 12
DP  - 2017 Dec
TI  - Photoacoustic imaging for in vivo quantification of placental oxygenation in 
      mice.
PG  - 5520-5529
LID - 10.1096/fj.201700047RR [doi]
AB  - Accurate analysis of placental and fetal oxygenation is critical during 
      pregnancy. Photoacoustic imaging (PAI) combines laser technology with ultrasound 
      in real time. We tested the sensitivity and accuracy of PAI for analysis of 
      placental and fetal oxygen saturation (sO(2)) in mice. The placental labyrinth 
      (L) had a higher sO(2) than the junctional zone plus decidua region (JZ+D) in 
      C57Bl/6 mice. Changing maternal O(2) from 100 to 20% in C57Bl/6 mice lowered 
      sO(2) in these regions. C57Bl/6 mice were treated with the NO synthase inhibitor 
      L-N(G)-nitroarginine methyl ester (L-NAME) from gestational day (GD) 11 to GD18 
      to induce hypertension. L-NAME decreased sO(2) in L and JZ+D at GD14 and GD18 in 
      association with fetal growth restriction and higher blood pressure. 
      Hypoxia-inducible factor 1alpha immunostaining was higher in L-NAME vs control mice 
      at GD14. Fetal sO(2) levels were similar between l-NAME and control mice at GD14 
      and GD18. In contrast to untreated C57Bl/6, L-NAME decreased placental sO(2) at 
      GD14 and GD18 vs GD10 or GD12. Placental sO(2) was lower in fetal growth 
      restriction in an angiotensin-converting enzyme 2 knockout mouse model 
      characterized by placental hypoxia. On phantom studies, patterns of sO(2) 
      measured directly correlated with those measured by PAI. In summary, PAI enables 
      the detection of placental and fetal oxygenation during normal and pathologic 
      pregnancies in mice.-Yamaleyeva, L. M., Sun, Y., Bledsoe, T., Hoke, A., Gurley, 
      S. B., Brosnihan, K. B. Photoacoustic imaging for in vivo quantification of 
      placental oxygenation in mice.
CI  - (c) FASEB.
FAU - Yamaleyeva, Liliya M
AU  - Yamaleyeva LM
AD  - Department of Surgery/Hypertension and Vascular Research, Wake Forest School of 
      Medicine, Winston-Salem, North Carolina, USA; lyamaley@wakehealth.edu.
FAU - Sun, Yao
AU  - Sun Y
AD  - Department of Radiology, Wake Forest School of Medicine, Winston-Salem, North 
      Carolina, USA.
FAU - Bledsoe, Tiffaney
AU  - Bledsoe T
AD  - Department of Surgery/Hypertension and Vascular Research, Wake Forest School of 
      Medicine, Winston-Salem, North Carolina, USA.
FAU - Hoke, Asia
AU  - Hoke A
AD  - Department of Surgery/Hypertension and Vascular Research, Wake Forest School of 
      Medicine, Winston-Salem, North Carolina, USA.
FAU - Gurley, Susan B
AU  - Gurley SB
AD  - Division of Nephrology, Department of Medicine, Durham Veterans Affairs and Duke 
      University Medical Centers, Durham, North Carolina, USA.
FAU - Brosnihan, K Bridget
AU  - Brosnihan KB
AD  - Department of Surgery/Hypertension and Vascular Research, Wake Forest School of 
      Medicine, Winston-Salem, North Carolina, USA.
LA  - eng
GR  - R21 HD086357/HD/NICHD NIH HHS/United States
GR  - R25 HL092618/HL/NHLBI NIH HHS/United States
GR  - S10 OD012330/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170821
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - S88TT14065 (Oxygen)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Female
MH  - Fetal Growth Retardation/metabolism
MH  - Hypertension/chemically induced/metabolism
MH  - Hypoxia/metabolism
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Oxygen/*metabolism
MH  - Photoacoustic Techniques/*methods
MH  - Placenta/drug effects/*metabolism
MH  - Pregnancy
PMC - PMC5690392
OTO - NOTNLM
OT  - HIF-1alpha
OT  - fetal growth restriction
OT  - hypertensive pregnancy
OT  - oxygen saturation
OT  - placental hypoxia
EDAT- 2017/08/27 06:00
MHDA- 2017/12/12 06:00
PMCR- 2018/12/01
CRDT- 2017/08/27 06:00
PHST- 2017/01/18 00:00 [received]
PHST- 2017/08/07 00:00 [accepted]
PHST- 2017/08/27 06:00 [pubmed]
PHST- 2017/12/12 06:00 [medline]
PHST- 2017/08/27 06:00 [entrez]
PHST- 2018/12/01 00:00 [pmc-release]
AID - fj.201700047RR [pii]
AID - FJ_201700047RR [pii]
AID - 10.1096/fj.201700047RR [doi]
PST - ppublish
SO  - FASEB J. 2017 Dec;31(12):5520-5529. doi: 10.1096/fj.201700047RR. Epub 2017 Aug 
      21.