PMID- 28842625
OWN - NLM
STAT- MEDLINE
DCOM- 20190604
LR  - 20190604
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Aug 25
TI  - Umbilical cord-derived mesenchymal stem cell extracts reduce colitis in mice by 
      re-polarizing intestinal macrophages.
PG  - 9412
LID - 10.1038/s41598-017-09827-5 [doi]
LID - 9412
AB  - Human umbilical cord mesenchymal stem cells (hUC-MSCs), originating in Wharton's 
      jelly, are multipotent stem cells that home to damaged tissues and can modulate 
      the immune system. We examined whether administering extracts of MSCs (MSC-Ex) 
      instead of MSCs could augment the beneficial effects of MSC therapy by overcoming 
      the low homing efficiency of MSCs systemically administered in inflammatory bowel 
      diseases (IBD). Dextran sodium sulfate-induced colitis model was established in 
      C57BL/6 mice, and MSC-Ex was administered intraperitoneally. MSC-Ex reduced 
      colitis, disease activity index (DAI), and histological colitis scores, and 
      increased the body weight. Treatment with MSC-Ex completely blocked the induction 
      of inflammatory cytokines, which were strongly detected in mice with colitis. 
      MSC-Ex shifted the macrophage functional phenotype from M1 to M2 by decreasing 
      the levels of MCP1, CXCL9, and iNOS, but increasing the levels of IL-10, LIGHT, 
      CCL1, and Arg-1. MSC-Ex recovered the destruction of the epithelial barrier in 
      the differentiated Caco-2 cells in vitro. Treatment with MSC-Ex was more potent 
      than that with MSC in reducing DAI, the histological score, and nitrite levels. 
      These data strongly support that MSC-Ex treatment can be a potent approach to 
      overcome severe refractory IBD.
FAU - Song, Ji-Young
AU  - Song JY
AD  - Institute for Life Science, University of Ulsan College of Medicine, Asan Medical 
      Center, Seoul, Korea.
FAU - Kang, Hyo Jeong
AU  - Kang HJ
AD  - Department of Pathology, University of Ulsan College of Medicine, Asan Medical 
      Center, Seoul, Korea.
AD  - Department of Physiology, Asan-Minnesota Institute for Innovating 
      Transplantation, Bio-Medical Institute of Technology, University of Ulsan College 
      of Medicine, Asan Medical Center, Seoul, Korea.
FAU - Hong, Joon Seok
AU  - Hong JS
AD  - Department of Obstetrics and Gynecology, Seoul National University Bundang 
      Hospital, Gyeonggi-do, Korea.
FAU - Kim, Chong Jai
AU  - Kim CJ
AD  - Department of Pathology, University of Ulsan College of Medicine, Asan Medical 
      Center, Seoul, Korea.
FAU - Shim, Jae-Yoon
AU  - Shim JY
AD  - Department Obstetrics and Gynecology, University of Ulsan College of Medicine, 
      Asan Medical Center, Seoul, Korea.
FAU - Lee, Christopher W
AU  - Lee CW
AD  - Department of Molecular and Cellular Biology, University of California, Davis, 
      Davis, California, USA.
FAU - Choi, Jene
AU  - Choi J
AD  - Department of Pathology, University of Ulsan College of Medicine, Asan Medical 
      Center, Seoul, Korea. jenec@amc.seoul.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170825
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cytokines)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Caco-2 Cells
MH  - Cell Differentiation
MH  - Colitis/*chemically induced/pathology/*therapy
MH  - Cytokines/metabolism
MH  - Dextran Sulfate/toxicity
MH  - Humans
MH  - Intestines/cytology
MH  - *Macrophage Activation
MH  - Macrophages/physiology
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/cytology
MH  - Mice, Inbred C57BL
MH  - Umbilical Cord
PMC - PMC5573412
COIS- The authors declare that they have no competing interests.
EDAT- 2017/08/27 06:00
MHDA- 2019/06/05 06:00
PMCR- 2017/08/25
CRDT- 2017/08/27 06:00
PHST- 2017/05/02 00:00 [received]
PHST- 2017/07/31 00:00 [accepted]
PHST- 2017/08/27 06:00 [entrez]
PHST- 2017/08/27 06:00 [pubmed]
PHST- 2019/06/05 06:00 [medline]
PHST- 2017/08/25 00:00 [pmc-release]
AID - 10.1038/s41598-017-09827-5 [pii]
AID - 9827 [pii]
AID - 10.1038/s41598-017-09827-5 [doi]
PST - epublish
SO  - Sci Rep. 2017 Aug 25;7(1):9412. doi: 10.1038/s41598-017-09827-5.