PMID- 28842886
OWN - NLM
STAT- MEDLINE
DCOM- 20180427
LR  - 20201209
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 1624
DP  - 2017
TI  - Multilocus Imaging of the E. coli Chromosome by Fluorescent In Situ 
      Hybridization.
PG  - 213-226
LID - 10.1007/978-1-4939-7098-8_16 [doi]
AB  - Fluorescence in situ hybridization (FISH) is a widely used technique to detect 
      and localize specific DNA or RNA sequences in cells. Although supplanted in many 
      ways by fluorescently labeled DNA binding proteins, FISH remains the only 
      cytological method to examine many genetic loci at once (up to six), and can be 
      performed in any cell type and genotype. These advantages have proved invaluable 
      in studying the spatial relationships between chromosome regions and the dynamics 
      of chromosome segregation in bacteria. A detailed protocol for DNA FISH in E. 
      coli is described.
FAU - Visser, Bryan J
AU  - Visser BJ
AD  - Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, 
      Houston, TX, USA.
FAU - Joshi, Mohan C
AU  - Joshi MC
AD  - Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, 
      TX, USA.
FAU - Bates, David
AU  - Bates D
AD  - Integrative Molecular and Biomedical Sciences, Baylor College of Medicine, 
      Houston, TX, USA. bates@bcm.edu.
AD  - Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, 
      TX, USA. bates@bcm.edu.
LA  - eng
GR  - R01 GM102679/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosome Segregation
MH  - Chromosomes, Bacterial/*genetics
MH  - Cytogenetic Analysis
MH  - Escherichia coli/*genetics
MH  - Genetic Loci
MH  - In Situ Hybridization, Fluorescence/*methods
PMC - PMC7000180
MID - NIHMS945959
OTO - NOTNLM
OT  - Bacterial chromosomes
OT  - E. coli chromosome
OT  - FISH
OT  - Fluorescence in situ hybridization
EDAT- 2017/08/27 06:00
MHDA- 2018/04/28 06:00
PMCR- 2020/02/04
CRDT- 2017/08/27 06:00
PHST- 2017/08/27 06:00 [entrez]
PHST- 2017/08/27 06:00 [pubmed]
PHST- 2018/04/28 06:00 [medline]
PHST- 2020/02/04 00:00 [pmc-release]
AID - 10.1007/978-1-4939-7098-8_16 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1624:213-226. doi: 10.1007/978-1-4939-7098-8_16.