PMID- 28843892 OWN - NLM STAT- MEDLINE DCOM- 20180620 LR - 20180620 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 210 DP - 2018 Jan 10 TI - Anti-inflammatory effect of stem bark of Paulownia tomentosa Steud. in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and LPS-induced murine model of acute lung injury. PG - 23-30 LID - S0378-8741(17)31103-0 [pii] LID - 10.1016/j.jep.2017.08.028 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: The leaves, bark, and flowers of Paulownia tomentosa Steud. have been widely used as a traditional medicine in East Asia to treat inflammatory and infectious diseases. AIM OF THE STUDY: We investigated the protective effect of the methanol stem bark extract of P. tomentosa using an animal model of lipopolysaccharide (LPS)-induced acute lung injury (ALI). MATERIALS AND METHODS: The UPLC Q-TOF-MS profiles for the methanol extract of P. tomentosa stem bark showed that verbascoside and isoverbascoside were the predominant compounds. Raw 264.7 cells were used for inhibitory effects of cytokine production in vitro. C57BL/6N mice were administered intranasally with LPS (10mug/per mouse) to induce ALI. H&E staining was used to evaluate histological changes in the lung. RESULTS: Treatment with P. tomentosa stem bark extract (PTBE) suppressed the production of IL-6 and TNF-alpha in LPS-stimulated RAW 264.7 macrophages, and the recruitment of neutrophils and macrophages in the BALF of mice with LPS-induced ALI. PTBE also decreased the levels of reactive oxygen species (ROS) and pro-inflammatory cytokines in the BALF. PTBE reduced the levels of nitric oxide (NO) in the serum and of inducible nitric oxide synthase (iNOS) in the lung of ALI mice. PTBE also attenuated the infiltration of inflammatory cells and the expression of monocyte chemoattractant protein-1 (MCP-1) in the lung. In addition, PTBE suppressed the activation of NF-kappaB and the reduced expression of superoxide dismutase 3 (SOD3) in the lung. CONCLUSION: The results suggest that PTBE has a protective effect on LPS-induced ALI. CI - Copyright (c) 2017 Elsevier Ireland Ltd. All rights reserved. FAU - Lee, Jae-Won AU - Lee JW AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea. Electronic address: suc369@kribb.re.kr. FAU - Seo, Kyeong-Hwa AU - Seo KH AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea. Electronic address: kyeonghwseo@korea.kr. FAU - Ryu, Hyung Won AU - Ryu HW AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea. Electronic address: cupid6@kribb.re.kr. FAU - Yuk, Heung Joo AU - Yuk HJ AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea. Electronic address: ryuhw@kribb.re.kr. FAU - Park, Hyun Ah AU - Park HA AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea. Electronic address: hyunah00@kribb.re.kr. FAU - Lim, YouRim AU - Lim Y AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea. Electronic address: youlim@kribb.re.kr. FAU - Ahn, Kyung-Seop AU - Ahn KS AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea. Electronic address: ksahn@kribb.re.kr. FAU - Oh, Sei-Ryang AU - Oh SR AD - Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Chungju-si, Chungbuk 363-883, Republic of Korea. Electronic address: seiryang@kribb.re.kr. LA - eng PT - Journal Article DEP - 20170824 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Anti-Inflammatory Agents) RN - 0 (Cytokines) RN - 0 (Lipopolysaccharides) RN - 0 (NF-kappa B) RN - 0 (Plant Extracts) RN - 0 (Reactive Oxygen Species) RN - 31C4KY9ESH (Nitric Oxide) RN - EC 1.14.13.39 (Nitric Oxide Synthase Type II) SB - IM MH - Acute Lung Injury/*drug therapy/pathology MH - Animals MH - Anti-Inflammatory Agents/isolation & purification/*pharmacology MH - Cytokines/metabolism MH - Disease Models, Animal MH - Inflammation/*drug therapy/pathology MH - Lipopolysaccharides/administration & dosage MH - Macrophages/drug effects MH - Male MH - Medicine, East Asian Traditional MH - Mice MH - Mice, Inbred C57BL MH - NF-kappa B/metabolism MH - Nitric Oxide/blood MH - Nitric Oxide Synthase Type II/metabolism MH - Plant Extracts/isolation & purification/*pharmacology MH - RAW 264.7 Cells MH - Reactive Oxygen Species/metabolism MH - Scrophulariaceae/*chemistry OTO - NOTNLM OT - ALI NF-kappaB OT - Anti-inflammatory effect OT - Isoverbascoside (PubChem CID: 6476333) OT - Paulownia tomentosa stem bark extract OT - SOD3 OT - Verbascoside (PubChem CID: 5281800) EDAT- 2017/08/28 06:00 MHDA- 2018/06/21 06:00 CRDT- 2017/08/28 06:00 PHST- 2017/03/21 00:00 [received] PHST- 2017/08/09 00:00 [revised] PHST- 2017/08/23 00:00 [accepted] PHST- 2017/08/28 06:00 [pubmed] PHST- 2018/06/21 06:00 [medline] PHST- 2017/08/28 06:00 [entrez] AID - S0378-8741(17)31103-0 [pii] AID - 10.1016/j.jep.2017.08.028 [doi] PST - ppublish SO - J Ethnopharmacol. 2018 Jan 10;210:23-30. doi: 10.1016/j.jep.2017.08.028. Epub 2017 Aug 24.