PMID- 28844046
OWN - NLM
STAT- MEDLINE
DCOM- 20171102
LR  - 20181202
IS  - 1468-2079 (Electronic)
IS  - 0007-1161 (Linking)
VI  - 101
IP  - 11
DP  - 2017 Nov
TI  - Paracrine effects of mesenchymal stem cells on the activation of keratocytes.
PG  - 1583-1590
LID - 10.1136/bjophthalmol-2016-310012 [doi]
AB  - AIMS: The purpose of this study is to investigate the impact of mesenchymal stem 
      cell (MSC)-derived soluble factors on the function of keratocytes, with a 
      particular focus on the processes involved in wound healing, including keratocyte 
      activation, migration and proliferation as well as extracellular matrix (ECM) 
      synthesis. METHODS: Primary cultured rabbit keratocytes were treated with 
      MSC-conditioned medium (MSC-CM). The paracrine factors released by bone marrow 
      MSCs were examined by ELISA. Time-lapse microscope was used to examine wound 
      closure in vitro. Mouse model of corneal injury was made by epithelial scraping 
      after ethanol injury. RESULTS: MSC-CM significantly increased the wound closure 
      rate of corneal stromal cells in vitro. This enhancement of wound closure by 
      MSC-CM was due to the promotion of cell migration. MSC-CM enhanced keratocyte 
      survival following ethanol injury via inhibiting apoptosis. The expression of ECM 
      component genes in keratocytes was upregulated by MSC-CM. In addition, MSC-CM 
      promoted corneal epithelial wound healing following chemical injury. A number of 
      wound healing mediators were detected in MSC-CM, including vascular endothelial 
      growth factor (VEGF), platelet-derived growth factor (PDGF), hepatocyte growth 
      factor (HGF), transforming growth factor beta 1 (TGFbeta1), interleukin 8 (IL8), 
      interleukin 6 (IL6) and monocyte chemoattractant protein 1 (MCP1). CONCLUSION: 
      MSC secretes certain factors that accelerate corneal re-epithelialisation. The 
      paracrine effects of MSC on corneal wound healing including improvements in cell 
      viability, migration and ECM formation.
CI  - (c) Article author(s) (or their employer(s) unless otherwise stated in the text of 
      the article) 2017. All rights reserved. No commercial use is permitted unless 
      otherwise expressly granted.
FAU - Jiang, Zhixin
AU  - Jiang Z
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
AD  - Nankai University Eye Hospital, Tianjin, China.
AD  - Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and 
      Visual Science, Tianjin, China.
FAU - Liu, Guangjie
AU  - Liu G
AD  - Department of Respiratory Medicine, Beijing Tongren Hospital, Capital Medical 
      University, Beijing, China.
FAU - Meng, Fanlan
AU  - Meng F
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
FAU - Wang, Weiyi
AU  - Wang W
AD  - Department of Ophthalmology, Tianjin Huanhu Hospital, Tianjin, China.
FAU - Hao, Peng
AU  - Hao P
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
AD  - Nankai University Eye Hospital, Tianjin, China.
AD  - Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and 
      Visual Science, Tianjin, China.
FAU - Xiang, Yang
AU  - Xiang Y
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
FAU - Wang, Yuchuan
AU  - Wang Y
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
AD  - Nankai University Eye Hospital, Tianjin, China.
AD  - Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and 
      Visual Science, Tianjin, China.
FAU - Han, RuiFang
AU  - Han R
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
AD  - Nankai University Eye Hospital, Tianjin, China.
AD  - Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and 
      Visual Science, Tianjin, China.
FAU - Li, Fang
AU  - Li F
AD  - Nankai University Eye Hospital, Tianjin, China.
FAU - Wang, Liming
AU  - Wang L
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
AD  - Nankai University Eye Hospital, Tianjin, China.
AD  - Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and 
      Visual Science, Tianjin, China.
FAU - Li, Xuan
AU  - Li X
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
AD  - Nankai University Eye Hospital, Tianjin, China.
AD  - Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and 
      Visual Science, Tianjin, China.
LA  - eng
PT  - Journal Article
DEP - 20170826
PL  - England
TA  - Br J Ophthalmol
JT  - The British journal of ophthalmology
JID - 0421041
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Cell- and Tissue-Based Therapy/*methods
MH  - Cells, Cultured
MH  - Corneal Injuries/*metabolism/pathology/therapy
MH  - Corneal Keratocytes/metabolism/*pathology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Extracellular Matrix/metabolism
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Rabbits
MH  - *Wound Healing
OTO - NOTNLM
OT  - cornea
OT  - stem cells
OT  - wound healing
COIS- Competing interests: None declared.
EDAT- 2017/08/28 06:00
MHDA- 2017/11/03 06:00
CRDT- 2017/08/28 06:00
PHST- 2016/12/07 00:00 [received]
PHST- 2017/06/20 00:00 [revised]
PHST- 2017/07/30 00:00 [accepted]
PHST- 2017/08/28 06:00 [pubmed]
PHST- 2017/11/03 06:00 [medline]
PHST- 2017/08/28 06:00 [entrez]
AID - bjophthalmol-2016-310012 [pii]
AID - 10.1136/bjophthalmol-2016-310012 [doi]
PST - ppublish
SO  - Br J Ophthalmol. 2017 Nov;101(11):1583-1590. doi: 
      10.1136/bjophthalmol-2016-310012. Epub 2017 Aug 26.