PMID- 28844736
OWN - NLM
STAT- MEDLINE
DCOM- 20180208
LR  - 20180917
IS  - 1570-9639 (Print)
IS  - 1570-9639 (Linking)
VI  - 1865
IP  - 11 Pt A
DP  - 2017 Nov
TI  - Structure and binding studies of proliferating cell nuclear antigen from 
      Leishmania donovani.
PG  - 1395-1405
LID - S1570-9639(17)30192-9 [pii]
LID - 10.1016/j.bbapap.2017.08.011 [doi]
AB  - Proliferating cell nuclear antigen (PCNA) acts as a sliding clamp to support DNA 
      replication and repair. The structure of PCNA from Leishmania donovani (LdPCNA) 
      has been determined at 2.73A resolution. Structure consists of six 
      crystallographically independent molecules which form two trimeric rings. The 
      pore diameter of the individual trimeric ring is of the order of 37A. The two 
      rings are stacked through their front to front faces. In order to gain a stable 
      packing, the rings are rotated by 42 degrees  about the pore axis and shifted by 7A and 
      tilted by 16 degrees  along the perpendicular direction to pore axis. This form of 
      stacking reduced the effective diameter of the pore to 32A. The sequence of 
      LdPCNA consists of a long segment of 41 amino acid residues 
      (186-Gly-Val-Ser-Asp-Arg-Ser-Thr-Lys-Ser-Glu-Val-Lys-Ala-Glu-Val-Lys-Ala-Glu-Ala-Arg-Asp-Asp-Asp-Glu-Glu-Pro-Leu-Ser-Arg-Lys-Tyr-Gly-Lys-Ala-Asp-Ser-Ser-Ala-Asn-Ala-Ile-226) 
      whereas the corresponding segments in other PCNAs contain only eight residues 
      corresponding to 186-Gly-Val-Ser-Asp-Arg------224-Asn-Ala-Ile-226. The enhanced 
      length of this segment in LdPCNA may influence its mode of interaction with DNA 
      and other proteins. The dissociation constants obtained using real time binding 
      studies with surface plasmon resonance (SPR) for two peptides, 
      Lys-Arg-Arg-Gln-Thr-Ser-Met-Thr-Asp-Phe-Tyr-His (P1) from human cyclin-dependent 
      kinase inhibitor-1(CKI-1) and Lys-Thr-Gln-Gly-Arg-Leu-Asp-Ser-Phe-Phe-Thr-Val 
      (P2) from flap endonuclease 1 (Fen-1) as well as with two small molecule 
      inhibitors, (S)-4-(4-(2-amino-3-hydroxypropyl)-2, 6-diiodophenoxy) phenol 
      hydrochloride (ADPH) and 
      N-(3-methylthiophene-2-carboxylicacid)-N'-((3-hydroxy-2-naphthalenyl) methylene) 
      hydrazide (MCMH) are 0.29+/-0.09muM, 0.37+/-0.08muM, 0.35+/-0.09muM and 1.20+/-0.08muM 
      respectively. The corresponding values obtained using fluorescence spectroscopic 
      methods were 0.22+/-0.06muM, 0.68+/-0.07muM, 0.44+/-0.07muM and 0.75+/-0.05muM respectively.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Yadav, Satya Prakash
AU  - Yadav SP
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Singh, Prashant Kumar
AU  - Singh PK
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Sharma, Pradeep
AU  - Sharma P
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Iqbal, Naseer
AU  - Iqbal N
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Kaur, Punit
AU  - Kaur P
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Sharma, Sujata
AU  - Sharma S
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Singh, Tej P
AU  - Singh TP
AD  - Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 
      India. Electronic address: tpsingh.aiims@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170824
PL  - Netherlands
TA  - Biochim Biophys Acta Proteins Proteom
JT  - Biochimica et biophysica acta. Proteins and proteomics
JID - 101731734
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (DNA, Protozoan)
RN  - 0 (Phenols)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.1.- (Flap Endonucleases)
RN  - EC 3.1.11.- (FEN1 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Cloning, Molecular
MH  - Crystallography, X-Ray
MH  - Cyclin-Dependent Kinase Inhibitor p21/chemistry
MH  - DNA, Protozoan/*chemistry/genetics/metabolism
MH  - Escherichia coli/genetics/metabolism
MH  - Flap Endonucleases/chemistry
MH  - Gene Expression
MH  - Leishmania donovani/*chemistry/metabolism
MH  - Models, Molecular
MH  - Phenols/chemistry
MH  - Proliferating Cell Nuclear Antigen/*chemistry/genetics/metabolism
MH  - Protein Binding
MH  - Protein Conformation, alpha-Helical
MH  - Protein Conformation, beta-Strand
MH  - Protein Interaction Domains and Motifs
MH  - Protein Multimerization
MH  - Protozoan Proteins/antagonists & inhibitors/*chemistry/genetics/metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
OTO - NOTNLM
OT  - Dimer of a homotrimer
OT  - Homotrimer
OT  - Pore-size
OT  - Reduction in effective diameter
OT  - Twisted stacking of trimers
EDAT- 2017/08/29 06:00
MHDA- 2018/02/09 06:00
CRDT- 2017/08/29 06:00
PHST- 2017/05/08 00:00 [received]
PHST- 2017/08/07 00:00 [revised]
PHST- 2017/08/10 00:00 [accepted]
PHST- 2017/08/29 06:00 [pubmed]
PHST- 2018/02/09 06:00 [medline]
PHST- 2017/08/29 06:00 [entrez]
AID - S1570-9639(17)30192-9 [pii]
AID - 10.1016/j.bbapap.2017.08.011 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Proteins Proteom. 2017 Nov;1865(11 Pt A):1395-1405. doi: 
      10.1016/j.bbapap.2017.08.011. Epub 2017 Aug 24.