PMID- 28848992
OWN - NLM
STAT- MEDLINE
DCOM- 20180515
LR  - 20231213
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 40
IP  - 4
DP  - 2017 Oct
TI  - Antimicrobial activity and synergism of ursolic acid 3-O-alpha-L-arabinopyranoside 
      with oxacillin against methicillin-resistant Staphylococcus aureus.
PG  - 1285-1293
LID - 10.3892/ijmm.2017.3099 [doi]
AB  - The objective of the present study was to investigate the antibacterial activity 
      of a single constituent, ursolic acid 3-O-alpha-L-arabinopyranoside (URS), isolated 
      from the leaves of Acanthopanax henryi (Oliv.) Harms, alone and in combination 
      with oxacillin (OXA) against methicillin-resistant Staphylococcus aureus (MRSA). 
      A broth microdilution assay was used to determine the minimal inhibitory 
      concentration (MIC). The synergistic effects of URS and OXA were determined using 
      a checkerboard dilution test and time-kill curve assay. The mechanism of action 
      of URS against MRSA was analyzed using a viability assay in the presence of a 
      detergent and an ATPase inhibitor. Morphological changes in the URS-treated MRSA 
      strains were evaluated via transmission electron microscopy (TEM). In addition, 
      the producing penicillin-binding protein 2a (PBP2a) protein level was analyzed 
      using western blotting. The MIC value of URS against MRSA was found to be 
      6.25 microg/ml and there was a partial synergistic effect between OXA and URS. The 
      time-kill growth curves were suppressed by OXA combined with URS at a 
      sub-inhibitory level. Compared to the optical density at 600 nm (OD600) value of 
      URS alone (0.09 microg/ml), the OD600 values of the suspension in the presence of 
      0.09 microg/ml URS and 0.00001% Triton X-100 or 250 microg/ml 
      N,N'-dicyclohexylcarbodiimide reduced by 56.6 and 85.9%, respectively. The TEM 
      images of MRSA indicated damage to the cell wall, broken cell membranes and cell 
      lysis following treatment with URS and OXA. Finally, an inhibitory effect on the 
      expression of PBP2a protein was observed when cells were treated with URS and OXA 
      compared with untreated controls. The present study suggested that URS was 
      significantly active against MRSA infections and revealed the potential of URS as 
      an effective natural antibiotic.
FAU - Zhou, Tian
AU  - Zhou T
AD  - Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental 
      Medicines Research Institute, Institute of Biotechnology, Wonkwang University, 
      Iksan, Jeonbuk 570-749, Republic of Korea.
FAU - Li, Zhi
AU  - Li Z
AD  - School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 
      410-208, P.R. China.
FAU - Kang, Ok-Hwa
AU  - Kang OH
AD  - Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental 
      Medicines Research Institute, Institute of Biotechnology, Wonkwang University, 
      Iksan, Jeonbuk 570-749, Republic of Korea.
FAU - Mun, Su-Hyun
AU  - Mun SH
AD  - Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental 
      Medicines Research Institute, Institute of Biotechnology, Wonkwang University, 
      Iksan, Jeonbuk 570-749, Republic of Korea.
FAU - Seo, Yun-Soo
AU  - Seo YS
AD  - Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental 
      Medicines Research Institute, Institute of Biotechnology, Wonkwang University, 
      Iksan, Jeonbuk 570-749, Republic of Korea.
FAU - Kong, Ryong
AU  - Kong R
AD  - Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental 
      Medicines Research Institute, Institute of Biotechnology, Wonkwang University, 
      Iksan, Jeonbuk 570-749, Republic of Korea.
FAU - Shin, Dong-Won
AU  - Shin DW
AD  - Department of Oriental Medicine Resources, College of Bio Industry Science, 
      Sunchon National University, Sunchon, Jeonnam 540-742, Republic of Korea.
FAU - Liu, Xiang-Qian
AU  - Liu XQ
AD  - School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 
      410-208, P.R. China.
FAU - Kwon, Dong-Yeul
AU  - Kwon DY
AD  - Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental 
      Medicines Research Institute, Institute of Biotechnology, Wonkwang University, 
      Iksan, Jeonbuk 570-749, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20170814
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Drug Combinations)
RN  - 0 (Glycosides)
RN  - 0 (Plant Extracts)
RN  - 0 (Triterpenes)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Anti-Bacterial Agents/isolation & purification/*pharmacology
MH  - Cell Wall/*drug effects/metabolism/ultrastructure
MH  - Drug Combinations
MH  - Drug Synergism
MH  - Eleutherococcus/chemistry
MH  - Glycosides/isolation & purification/*pharmacology
MH  - Methicillin-Resistant Staphylococcus aureus/*drug effects/growth & 
      development/metabolism/ultrastructure
MH  - Microbial Sensitivity Tests
MH  - Microscopy, Electron, Transmission
MH  - Oxacillin/*pharmacology
MH  - Plant Extracts/chemistry
MH  - Plant Leaves/chemistry
MH  - Triterpenes/isolation & purification/*pharmacology
MH  - Ursolic Acid
EDAT- 2017/08/30 06:00
MHDA- 2018/05/16 06:00
CRDT- 2017/08/30 06:00
PHST- 2017/02/07 00:00 [received]
PHST- 2017/07/28 00:00 [accepted]
PHST- 2017/08/30 06:00 [pubmed]
PHST- 2018/05/16 06:00 [medline]
PHST- 2017/08/30 06:00 [entrez]
AID - 10.3892/ijmm.2017.3099 [doi]
PST - ppublish
SO  - Int J Mol Med. 2017 Oct;40(4):1285-1293. doi: 10.3892/ijmm.2017.3099. Epub 2017 
      Aug 14.